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公开(公告)号：EP0196056A3
公开(公告)日：1987-12-23
申请号：EP86104066
申请日：1986-03-25
申请人： CHIRON CORPORATION
发明人： Cousens, Lawrence S. , Tekamp-Olson, Patricia A. , Shuster, Jeffrey R. , Merryweather, James P.
IPC分类号： C12N15/00 , C12P21/02 , C12N09/02 , G01N33/569
CPC分类号： C07K14/005 , C07K14/62 , C07K14/65 , C07K2319/00 , C07K2319/02 , C07K2319/50 , C07K2319/75 , C12N9/0089 , C12N15/81 , C12N2740/16222 , C12Y115/01001
摘要： 57 Novel methods and compositions are provided for enhanced yield of heterologous proteins in fungi. The method and compositions involve employing fusion sequences involving a sequence encoding a heterologous product produced in relatively large amount as a stable polypeptide in the host fused to a second sequence in open reading frame with the prior sequence coding for a different heterologous polypeptide, where the two polypeptides are joined by a selectively cleavable linkage. In particular, a sequence coding for superoxide dismutase is joined to another polypeptide of interest at either terminus of the superoxide dismutase in a yeast expression vector under transcriptional control of an active promoter and the vector introduced into a yeast host and the host grown. High yields of the fusion product are obtained in this manner, where the fusion product can be selectively cleaved so as to produce both the superoxide dismutase and the other polypeptide in high yield. The S. cerevisiae strains 2150-2-3 (pYASl1) and AB110 (pYLUIGF2-14) were deposited at the A.T.C.C. on February 27, 1985 and March 19,1986, respectively; and given accession nos. 20745 and 20796, respectively. CROSS-REFERENCE TO RELATED APPLICATIONS This application is a Continuation of application Serial No. 717 209, filed March 28, 1985, which disclosure is incorporated herein by reference.
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公开(公告)号：EP0098138A3
公开(公告)日：1986-03-05
申请号：EP83303697
申请日：1983-06-27
IPC分类号： C12N01/20 , C12N01/28 , C12N01/32 , C12P17/02 , C12P07/26 , C12P07/02 , C12N09/02 , C12R01/01 , C12R01/04 , C12R01/06 , C12R01/13 , C12R01/15 , C12R01/32 , C12R01/365 , C12R01/38
CPC分类号： C12N9/0006 , C12N1/20 , C12N1/28 , C12N1/30 , C12N1/32 , C12P7/02 , C12P7/06 , C12P7/16 , C12P7/22 , C12P7/26 , C12P7/28 , C12P17/02 , C12R1/01 , C12R1/04 , C12R1/06 , C12R1/13 , C12R1/15 , C12R1/32 , C12R1/365 , C12R1/38 , Y02E50/10 , Y02E50/17
摘要： Newly discovered and isolated microorganism strains and their natural mutants and genetically engineered derivatives are capable of growth under aerobic conditions in a culture medium containing a C 2 -C 10 alkane or a C 2 -C 10 alkyl radical donating compound as their major carbon and energy source. These microbial cells, which possess a high content of protein, can be utilized as feedstuffs or in oxidizing oxidizable organic substrates containing at least two carbon atoms, for example, converting alkanes and alicyclic and aromatic hydrocarbons to alcohols and/or ketones, alkenes, dienes, and vinyl aromatic compounds to 1,2-epoxides, and secondary alcohols to methyl ketones. Genetically engineered derivatives and enzyme preparations derived from these microbial cells may also be employed in the oxidative conversions.
摘要翻译： 新发现和分离的微生物菌株及其天然突变体和遗传工程化衍生物能够在有氧条件下在含有C2-C10烷烃或C2-C10烷基基团的化合物作为主要碳源和能源的培养基中生长。 具有高蛋白质含量的这些微生物细胞可用作饲料或氧化含有至少两个碳原子的可氧化有机底物，例如将烷烃和脂环族和芳族烃转化为醇和/或酮，烯烃，二烯 和乙烯基芳族化合物转化为1,2-环氧化物，和仲醇转化成甲基酮。 衍生自这些微生物细胞的基因工程衍生物和酶制剂也可用于氧化转化。
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23.发明公开
公开(公告)号：EP0112299A3
公开(公告)日：1986-01-29
申请号：EP83830118
申请日：1983-06-10
发明人： Bianchi, Ines
IPC分类号： C12N09/02
CPC分类号： C12N9/0089
摘要： Method for obtaining purified superoxidic-dismutasis from animal tissues, in particular from erythrocytes or from hepatic tissue, through affinity chromatography or immunoabsorbent with specific purified antibodies for the enzyme.
摘要翻译： 从动物组织，特别是红细胞或肝组织获得纯化的超氧化物歧化的方法，通过亲和色谱法或具有针对该酶的特异性纯化抗体的免疫吸收剂。
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公开(公告)号：EP0071446A3
公开(公告)日：1984-05-23
申请号：EP82303937
申请日：1982-07-26
申请人： CELANESE CORPORATION
发明人： Maxwell, Peter C.
摘要： This invention provides novel strains of microorganisms (e.g., Pseudomonas putida Biotype A) which are capable of converting toluene to muconic acid quantitatively by the ortho(β-ketoadipate) pathway. Muconate lactonising enzyme is not induced in the microorganism, thereby permitting the muconic acid to be produced and accumulated in a quantity greater than one gram of muconic acid per litre of growth medium.
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25.发明公开Immobilized superoxide dismutase, its production and pharmaceutical composition 失效固化超氧化物歧化酶及其制备和药物组合物
公开(公告)号：EP0070656A3
公开(公告)日：1983-08-10
申请号：EP82303600
申请日：1982-07-09
CPC分类号： C12N9/0089 , A61K38/446 , C12N11/02 , C12N11/10
摘要： A novel superoxide dismutase is produced by cultivating a microorganism belonging to the genus Serratia. The superoxide dismutase is useful as an antiinflammatory agent. An immobilized superoxide dismutase has properties of decreased antigenicity and increased antiinflammatory activity.
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公开(公告)号：EP0068582A3
公开(公告)日：1983-07-27
申请号：EP82200778
申请日：1982-06-23
摘要： The purification process of water containing organic pollutants involves the conveying of the water to be purified in a reactor (4) in which it undergoes a biochemical treatment with enzyme catalysis, when necessary combined with chemical and/or chemical-physical treatment.
摘要翻译： 含水有机污染物的净化过程涉及在反应器（4）中输送待纯化的水，在反应器（4）中进行生物化学处理，当需要与化学和/或化学物理处理相结合时，进行酶催化。
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公开(公告)号：EP0045222A3
公开(公告)日：1982-08-04
申请号：EP81303498
申请日：1981-07-30
IPC分类号： C12N09/02 , A61K37/50 , C12R01/425
CPC分类号： C12N9/0089 , A61K38/446
摘要： A novel superoxide dismutase is produced by cultivating a microorganism belonging to the genus Serratia. The superoxide dismutase is useful as an antiinflammatory agent.
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