Method for producing L-carnitine
    4.
    发明公开
    Method for producing L-carnitine 失效
    生产L-卡尼汀的方法

    公开(公告)号:EP0122794A3

    公开(公告)日:1986-07-02

    申请号:EP84302539

    申请日:1984-04-13

    CPC分类号: C12P13/007

    摘要: A method for producing L-camitine which comprises contacting crotonbetaine or a salt thereof with a microorganism or fraction of said microorganism which contains the enzymes capable of converting crotonbetaine or salts thereof into L-camitine in an aqueous medium under conditions suitable for the production of L-carnitine.

    Transposable cloning vector
    6.
    发明公开
    Transposable cloning vector 失效
    可传递的克隆向量

    公开(公告)号:EP0091723A3

    公开(公告)日:1985-12-18

    申请号:EP83300542

    申请日:1983-02-03

    CPC分类号: C12N15/00

    摘要: Novel transposable cloning vector and method of using it for the insertion into the chromosome of a receipient Gram-negative bacterium of DNA material foreign to that bacterium. The vector comprises first and second plasmids. The first plasmid carries a transposon (A), a part of whose DNA including the sequence which specifies its transposition function has been removed. The second plasmid carries a fragment of DNA from a transposon (A) including the sequence which specifies its transposition function. The DNA material to be inserted into the chromosome of the recipient bacterium is included in the DNA of transposon (A) carried by the first plasmid. Any suitable transposon may be used as transposon (A) but transposon Tn7 is preferred.