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    Stabilisation of a mutant micro-organism population
    1.
    发明公开
    Stabilisation of a mutant micro-organism population 失效
    突变微生物群体的稳定

    公开(公告)号：EP0098750A3

    公开(公告)日：1986-04-16

    申请号：EP83303840

    申请日：1983-07-01

    申请人： CELANESE CORPORATION

    发明人： Maxwell, Peter C. ,  Hsieh, Jih-Han ,  Fieschko, John C.

    IPC分类号： C12P01/00 ,  C12N15/00 ,  C12P07/44

    CPC分类号： C12P7/44 ,  C12N15/01 ,  C12P1/00 ,  Y10S435/877

    摘要： This invention provides a process for stabilising a population of a mutant micro-organism in a bioconversion system whereby growth of revertant cells is suppressed. The process involves limiting a nutrient which is essential for cell growth so that the cells selectively grow on a growth carbon source rather than on a non-growth carbon source which is present. The revertant cells being suppressed have a similar ability as the parent strain of the mutant micro-organism to grow on the non-growth carbon source in the bioconversion system. The non-growth carbon source in the bioconversion system is metabolised to an extracellular accumulating quantity of a desired metabolite, e.g., toluene is converted to muconic acid.

    Construction of microorganisms
    2.
    发明公开
    Construction of microorganisms 失效
    微生物的构建

    公开(公告)号：EP0071446A3

    公开(公告)日：1984-05-23

    申请号：EP82303937

    申请日：1982-07-26

    申请人： CELANESE CORPORATION

    发明人： Maxwell, Peter C.

    IPC分类号： C12N15/00 ,  C12N01/20 ,  C12P07/44 ,  C12R01/40 ,  C12N09/02

    CPC分类号： C12R1/40 ,  C12P7/44

    摘要： This invention provides novel strains of microorganisms (e.g., Pseudomonas putida Biotype A) which are capable of converting toluene to muconic acid quantitatively by the ortho(β-ketoadipate) pathway. Muconate lactonising enzyme is not induced in the microorganism, thereby permitting the muconic acid to be produced and accumulated in a quantity greater than one gram of muconic acid per litre of growth medium.