Specific chicken egg antibody and method for its production
    38.
    发明公开
    Specific chicken egg antibody and method for its production 失效
    SpezifischerHühnerei-Antikörperund Methode zu seiner Herstellung。

    公开(公告)号:EP0503293A1

    公开(公告)日:1992-09-16

    申请号:EP92102325.5

    申请日:1992-02-12

    IPC分类号: C07K15/06 A61K39/395

    摘要: The present invention is directed to a specific egg yolk antibody, which substantially has antibody activity against a particular antigen, produced by a process comprising of supercritical gas extraction from an egg yolk of hens immunized with the particular antigen, and to a process of producing such a specific egg yolk antibody. The present invention makes it possible to easily and cheaply produce a specific egg yolk antibody which substantially has antibody activity and sufficiently reduced levels of the color, odor and flavor of egg yolk. Also, the specific egg yolk antibody of the present invention is good in oxidation stability during storage and excellent in fluidity for the food processing.

    摘要翻译: 本发明涉及一种特定的蛋黄抗体,其基本上具有针对特定抗原的抗体活性,该蛋白质通过包含从用特定抗原免疫的鸡蛋黄蛋白提取超临界气体的方法产生,以及生产这种抗体的方法 一种特定的蛋黄抗体。 本发明使得可以容易且廉价地生产基本上具有抗体活性并充分降低蛋黄颜色,气味和风味水平的特定蛋黄抗体。 此外,本发明的特定蛋黄抗体在贮存期间的氧化稳定性好,食品加工的流动性优异。

    Method for production of sialic acid
    39.
    发明公开
    Method for production of sialic acid 失效
    Verfahren zur Herstellung vonSialsäure。

    公开(公告)号:EP0474410A2

    公开(公告)日:1992-03-11

    申请号:EP91307753.3

    申请日:1991-08-22

    IPC分类号: C07H13/04 C07H1/08

    CPC分类号: C07H13/04 C07H7/027

    摘要: The present invention is directed to a mehtod for producing crude sialic acid, comprising hydrolysis of a delipidated egg yolk and a method for producing high purity sialic acid, which comprises desalting a solution containing sialic acid obtainable by hydrolyzing a delipidated egg yolk,, adsorbing sialic acid to an anion exchange resin and then eluting said sialic acid.
    The present invention makes it possible to produce and purify sialic acid from delipidated egg yolk on an industrial scale.

    摘要翻译: 本发明涉及生产粗唾液酸的方法,包括脱脂蛋黄的水解和生产高纯度唾液酸的方法,该方法包括将含有唾液酸的溶液脱盐,所述唾液酸通过水解脱脂的蛋黄,吸附唾液酸 酸与阴离子交换树脂反应,然后洗脱所述唾液酸。 本发明使得可以从工业规模的脱脂蛋黄制备和纯化唾液酸。

    DNA FOR SHUTTLE VECTOR
    40.
    发明公开
    DNA FOR SHUTTLE VECTOR 失效
    DNSFÜRSHUTTLE-VECTOR。

    公开(公告)号:EP0278005A1

    公开(公告)日:1988-08-17

    申请号:EP87904941.9

    申请日:1987-07-24

    IPC分类号: C12N15/00 C12N1/20

    CPC分类号: C12N15/69 C12N15/85

    摘要: A DNA usable as vector for animal cells, a process for Its preparation, a recombinant plasmid containing the DNA, and transformant bacteria containing the plasmid. The DNA is a part of polyoma virus DNA, has a cleavage point by restriction enzyme BamHl at one end, and has cleavage points by restriction enzyme Kpnl at distances of about 0.06 kb and about 3.0 kb or about 3.1 kb from the above-described end, cleavage points by restriction enzyme Pvull at distances of about 0.6 kb and about 1.9 kb and about 2.1 kb, or about 2.0 kb and about 2.2 kb, a cleavage point by restriction enzyme Acc at a distance of about 0.7 kb or about 0.8 kb, a cleavage point by restriction enzyme Bgll at a distance of about 0.9 kb or about 1.0 kb, a cleavage point by restriction enzyme EcoRl at a distance of about 2.4 kb or about 2.5 kb, a cleavage point by restriction enzyme Hindlll at a distance of about 2.5 kb or about 2.6 kb, and a cleavage point by restriction enzyme Hincll at a distance of about 3.8 kb or about 3.9 kb.

    摘要翻译: 从小鼠F9细胞中分离形成多瘤病毒基因组的一部分的DNA。 指定为TYA-1的DNA具有如图所示的限制酶切割图,并从小鼠F9-28或F9-80细胞中分离。 指定为TYA-II(类似)的DNA在PvuII和AccI的切割点之间具有额外的0.1kb的DNA; TYA-II的总体积为5.4kb,TYA-II与小鼠F9-36细胞分离。 还要求的是包含这些DNA片段的质粒pTYA-1和pTYA-II,以及分别含有pTYA-1和pTYA-II的大肠杆菌转化体JM83-I和JM83-II。