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    VERFAHREN ZUR BESTIMMUNG DES METHYLIERUNGSGRADES VON BESTIMMTEN CYTOSINEN IN GENOMISCHER DNA IM SEQUENZKONTEXT 5'-CPG-3'
    31.
    发明授权
    VERFAHREN ZUR BESTIMMUNG DES METHYLIERUNGSGRADES VON BESTIMMTEN CYTOSINEN IN GENOMISCHER DNA IM SEQUENZKONTEXT 5'-CPG-3' 有权
    PROCEDURE决定某些甲基化的胞嘧啶到基因组DNA序列的程度在上下文中5'-CPG-3'

    公开(公告)号：EP1423528B1

    公开(公告)日：2008-01-02

    申请号：EP01980315.4

    申请日：2001-09-01

    申请人： Epigenomics AG

    发明人： OLEK, Alexander ,  PIEPENBROCK, Christian ,  BERLIN, Kurt ,  GÜTIG, David

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6827 ,  C12Q1/6883 ,  C12Q2600/154

    摘要： The invention relates to a method for detecting the degree of methylation of a defined cytosine in the sequence context 5′-CpG-3′ of a genomic DNA sample. The first stage involves chemically treating the genomic DNA in such a way that the cytosine bases, but not the 5-methylcytosine bases, are converted into uracil. Parts of the genomic DNA containing the defined cytosine are then amplified. The amplified parts are given a detectable mark and the extent of the hybridisation of the amplified parts on the two classes of oligonucleotides is then determined by detecting the mark of the amplified parts. The degree of methylation of the defined cytosine in the genomic DNA sample can be deduced on the basis of the relationship between the marks detected on the two classes of oligonucleotides following the hybridisation.

    VERFAHREN ZUR BESTIMMUNG DES METHYLIERUNGSGRADES VON BESTIMMTEN CYTOSINEN IN GENOMISCHER DNA IM SEQUENZKONTEXT 5'-CPG-3'
    33.
    发明公开
    VERFAHREN ZUR BESTIMMUNG DES METHYLIERUNGSGRADES VON BESTIMMTEN CYTOSINEN IN GENOMISCHER DNA IM SEQUENZKONTEXT 5'-CPG-3' 有权
    VERFAHREN ZUR BESTIMMUNG DES METHYLIERUNGSGRADES VON BESTIMPTEN CYTOSINEN IN GENOMISCHER DNA IM SEQUENZKONTEXT 5'-CPG-3'

    公开(公告)号：EP1423528A2

    公开(公告)日：2004-06-02

    申请号：EP01980315.4

    申请日：2001-09-01

    申请人： Epigenomics AG

    发明人： OLEK, Alexander ,  PIEPENBROCK, Christian ,  BERLIN, Kurt ,  GÜTIG, David

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6827 ,  C12Q1/6883 ,  C12Q2600/154

    摘要： The invention relates to a method for detecting the degree of methylation of a defined cytosine in the sequence context 5′-CpG-3′ of a genomic DNA sample. The first stage involves chemically treating the genomic DNA in such a way that the cytosine bases, but not the 5-methylcytosine bases, are converted into uracil. Parts of the genomic DNA containing the defined cytosine are then amplified. The amplified parts are given a detectable mark and the extent of the hybridisation of the amplified parts on the two classes of oligonucleotides is then determined by detecting the mark of the amplified parts. The degree of methylation of the defined cytosine in the genomic DNA sample can be deduced on the basis of the relationship between the marks detected on the two classes of oligonucleotides following the hybridisation.

    摘要翻译： 确定存在于DNA（I）的基因组样品中的基序5'-CpG-3'中的特定胞嘧啶的甲基化程度是新的。 确定存在于DNA（I）的基因组样品中的基序5'-CpG-3'中的特定胞嘧啶的甲基化程度是新的。 将样品化学处理以将胞嘧啶（C）转化为尿嘧啶，然后将含有目标C的（I）部分扩增以形成标记的扩增子（II）。 （II）与寡核苷酸和/或肽 - 核酸（PNA）寡聚体（A）的两个类别分别与至少一个成员杂交，并且从（II）上的标记确定与两个等级的杂交程度。 从与（A）两类杂交的标记的比例，计算甲基化程度。 还包括以下独立权利要求：（1）包含亚硫酸氢盐，用于扩增的引物寡核苷酸和/或（A））的试剂盒，优选固定在固相上，加上说明书; （2）在说明书中未给出的序列（1） - （40712）的化学预处理的DNA（B）至少包含18个碱基的核酸; （3）用于新方法的寡核苷酸或PNA寡聚体（OG），其包含能够与（B）杂交的至少9个核苷酸（nt）的序列; （4）OG组; （5）至少两个用于扩增至少一个（B）或其片段的引物寡核苷酸的组; （6）用于确定（B）中的C甲基化和/或单核苷酸多态性程度的寡核苷酸探针组，其包含至少10个OG; （7）通过将OG连接到固相，制备不同OG的载体结合阵列，用于分析与（B）的甲基化状态相关的疾病; 和（8）由（7）制成的阵列。

    METHOD AND NUCLEIC ACIDS FOR THE ANALYSIS OF ASTROCYTOMAS
    34.
    发明公开
    METHOD AND NUCLEIC ACIDS FOR THE ANALYSIS OF ASTROCYTOMAS 审中-公开
    方法和核酸用于分析星形细胞瘤

    公开(公告)号：EP1356099A2

    公开(公告)日：2003-10-29

    申请号：EP01962814.8

    申请日：2001-07-02

    申请人： Epigenomics AG

    发明人： OLEK, Alexander ,  PIEPENBROCK, Christian ,  BERLIN, Kurt

    IPC分类号： C12Q1/68

    CPC分类号： C07K14/82 ,  C07K14/4703 ,  C12Q1/6883 ,  C12Q1/6886 ,  C12Q2523/125 ,  C12Q2600/154 ,  C12Q2600/156

    摘要： The present invention relates to chemically modified genomic sequences, to oligonucleotides and/or PNA-oligomers for detecting the cytosine methylation state of genomic DNA, as well as to a method for ascertaining genetic and/or epigenetic parameters of genes for use in the characterisation, classification, differentiation, grading, staging, treatment and/or diagnosis of astrocytomas, or the predisposition to astrocytomas.

    摘要翻译： 本发明涉及化学修饰的基因组序列，用于检测基因组DNA的胞嘧啶甲基化状态的寡核苷酸和/或PNA寡聚物，以及用于确定用于表征的基因的遗传和/或表观遗传参数的方法， 分类，分化，分级，分期，治疗和/或诊断星形细胞瘤，或易患星形细胞瘤。

    VERFAHREN ZUR IDENTIFIKATION VON CYTOSIN-METHYLIERUNGSMUSTERN IN GENOMISCHEN DNA-PROBEN
    35.
    发明授权
    VERFAHREN ZUR IDENTIFIKATION VON CYTOSIN-METHYLIERUNGSMUSTERN IN GENOMISCHEN DNA-PROBEN 有权
    一种用于识别胞嘧啶甲基格局的基因组DNA样品

    公开(公告)号：EP1147228B1

    公开(公告)日：2003-10-15

    申请号：EP00908971.5

    申请日：2000-01-27

    申请人： Epigenomics AG

    发明人： OLEK, Alexander

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6837 ,  C12Q1/6827 ,  C12Q2563/167 ,  C12Q2535/131 ,  C12Q2523/125

    摘要： The invention relates to a method of identifying cytosine methylation patterns in genomic DNA samples. The inventive method comprises the following steps: a) chemically treating a genomic DNA sample in such a manner that the cytosine and the 5-methyl cytosine react differently and that the two products have a different base-pairing behavior in the duplex; b) enzymatically amplifying parts of the DNA sample treated in this manner; c) binding the amplified parts of the DNA sample treated in this manner to a surface; d) hybridizing a set of probes of different nucleic base sequences to the immobilized DNA samples, said respective base sequences containing at least once the dinucleotide sequence 5'-CpG-3'; e) removing the non-hybridized probes; f) analyzing the hybridized probes in a mass spectrometer, the position of the probes on the sample carrier allowing an allocation of the DNA sample to be hybridized; g) converting the peak-patterns obtained from the mass-specters to methylation patterns and matching the new data with a data library.

    DIAGNOSE VON MIT CD24 ASSOZIIERTEN KRANKHEITEN
    36.
    发明公开
    DIAGNOSE VON MIT CD24 ASSOZIIERTEN KRANKHEITEN 审中-公开
    与CD24相关疾病的诊断

    公开(公告)号：EP1305449A2

    公开(公告)日：2003-05-02

    申请号：EP01978272.1

    申请日：2001-08-02

    申请人： Epigenomics AG

    发明人： OLEK, Alexander ,  PIEPENBROCK, Christian ,  BERLIN, Kurt

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/68

    摘要： The invention relates to the chemically modified genomic sequence of the CD24 gene, to oligonucleotides oriented against the sequence and/or PNA oligomers for detecting the cytosine methylation status of the CD24 gene and to a method for determining genetic and/or epigenetic parameters of the CD24 gene.