公开(公告)号：EP0123304A3

公开(公告)日：1987-03-04

申请号：EP84104514

申请日：1984-04-19

申请人： Asahi Kasei Kogyo Kabushiki Kaisha ,  KOWA COMPANY, LTD.

发明人： Murakami, Akira ,  Yoshizaki, Hideo

IPC分类号： C12N09/96 ,  A61K37/54 ,  C12N09/64

CPC分类号： C12N9/6459 ,  A61K38/00 ,  A61K47/42 ,  C12N9/96 ,  C12Y304/21069 ,  G03C2001/0471 ,  Y10S530/808 ,  Y10S530/848

摘要： A method for stabilizing a tissue plasminogen activator which involves adding to an aqueous solution or powder containing a tissue plasminogen activator an effective amount of a purified gelatin is disclosed. A stable aqueous solution or powder which contains a tissue plasminogen activator and an effective amount of a purified gelatin is also disclosed. The method and composition eliminate the problem of adsorption of tissue plasminogen activator to various laboratory equipment, and also prevent the conversion of the single-chain tissue plasminogen activatgor into a double-chain tissue plasminogen activator.

公开(公告)号：EP0091664A3

公开(公告)日：1985-05-15

申请号：EP83103406

申请日：1983-04-07

申请人： MEITO SANGYO KABUSHIKI KAISHA

发明人： Higashi, Toshihiko ,  Kobayashi, Yoshinori Meito Sangyo K.K. ,  Iwasaki, Shinjiro

IPC分类号： C12N09/99 ,  C12N09/58 ,  C12N09/96 ,  A23C19/032

CPC分类号： A23C19/0326 ,  C12N9/58 ,  C12N9/96 ,  Y10S435/931

摘要： A method for increasing the milk coagulating activity of microbial rennet obtained from Mucor pusillus microorganisms, which comprises acylating the microbial rennet in an aqueous medium by contacting the microbial rennet with succinic anhydride at a temperature of about 0°C to about 40°C and a pH of about 4 to about 10 for a time sufficient to obtain the desired increase in milk coagulating activity; and improved microbial rennet having a low PA/MCA index and a high MCA index and obtained by the aforesaid method.

公开(公告)号：EP0044532A3

公开(公告)日：1982-02-10

申请号：EP81105592

申请日：1981-07-16

申请人： E.I. DU PONT DE NEMOURS AND COMPANY

发明人： Briggs, Anglis R.

IPC分类号： C12Q01/00 ,  C12Q01/50 ,  C12N09/96

CPC分类号： C12Q1/00 ,  C12N9/96 ,  C12Q1/50

摘要： There is provided a lyophilized product which contains monothioglycerol as the thiol-protector agent for sulfhydryl requiring enzymes. The solution to be lyophilized also contains a substantiallv completely delipified animal serum having low residual levels of enzyme activity.

公开(公告)号：EP0044432A3

公开(公告)日：1982-02-03

申请号：EP81104899

申请日：1981-06-24

申请人： Modrovich, Ivan Endre

发明人： Modrovich, Ivan Endre

IPC分类号： C12Q01/60 ,  C12Q01/00 ,  C12N09/96

CPC分类号： C12Q1/60 ,  C12N9/96

摘要： Stabilized enzymes useful in the diagnostic assay of total cholesterol are prepared by combining three separately prepared reagents: (1) a cholesterol esterase reagent; (2) cholesterol oxidase reagent; and (3) a saline diluent reagent. The cholesterol esterase reagent is prepared by dissolving a salt of cholic acid in a buffer solution providing a pH within the range of about 4 to about 9. To this solution is added a cholesterol esterase. The solution is then mixed with a polyhydroxy organic compound and TRITON-X-100. A cholesterol oxidase and a polyhydroxy organic compound are each dissolved in separate portions of buffer solution to make the cholesterol esterase reagent. The saline diluent solution is prepared by dissolving potassium iodide in water and adding a polyhydroxy organic compound. The resultant three reagents, when combined, provide a stabilized enzyme solution having utility in the total cholesterol assay of a serum sample. The combined reagents have utility in a total oxygen consumption method of analysis of total cholesterol, which analysis method is also disclosed.