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1.发明公开Microbial rennet having increased milk coagulating activity and method for production thereof 失效具有增加乳汁凝血活性的微生物网络及其生产方法
公开(公告)号：EP0091664A3
公开(公告)日：1985-05-15
申请号：EP83103406
申请日：1983-04-07
IPC分类号： C12N09/99 , C12N09/58 , C12N09/96 , A23C19/032
CPC分类号： A23C19/0326 , C12N9/58 , C12N9/96 , Y10S435/931
摘要： A method for increasing the milk coagulating activity of microbial rennet obtained from Mucor pusillus microorganisms, which comprises acylating the microbial rennet in an aqueous medium by contacting the microbial rennet with succinic anhydride at a temperature of about 0°C to about 40°C and a pH of about 4 to about 10 for a time sufficient to obtain the desired increase in milk coagulating activity; and improved microbial rennet having a low PA/MCA index and a high MCA index and obtained by the aforesaid method.
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公开(公告)号：EP0188312A3
公开(公告)日：1988-01-13
申请号：EP86300042
申请日：1986-01-06
申请人： CELLTECH LIMITED
发明人： Harris, Timothy John Roy , Docherty, Andrew James Penrose , Reynolds, John James , Murphy, Gillian
CPC分类号： C07K14/8146 , A61K38/00 , C07K2319/02
摘要： A process for producing a metalloproteinase inhibitor or a precursor thereof comprising culturing host cells transformed with a vector including a gene coding for the metalloproteinase inhibitor or the precursor and, if appropriate, cleaving the precursor to yield metalloproteinase inhibitor. The precursor may be a premetalloproteinase inhibitor or a fusion protein comprising a heterologous protein and a metalloproteinase inhibitor. The metalloproteinase inhibitor has application as the effective component in a pharmaceutical composition for the treatment of disease processes where accelerated breakdown of the extracellular organic matrix is observed.
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公开(公告)号：EP0075293A3
公开(公告)日：1983-09-14
申请号：EP82108604
申请日：1982-09-17
CPC分类号： C12N9/99 , C12Q1/34 , G01N2333/924
摘要： β-D-galactosidase catalyzed reactions can be terminated by inhibiting β-D-galalctosidase in a method in which β-D-qalactosidase is brought in contact with a chelating agent at an alkaline pH range other than the optimal pH range for β-D-galactosidase.
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4.发明公开
公开(公告)号：EP0194600A3
公开(公告)日：1988-03-16
申请号：EP86103035
申请日：1986-03-07
CPC分类号： C07K16/40 , B29L2031/7154
摘要： Die Erfindung betrifft die Enzymaktivität von humaner elpha-Amylase zu mehr als 90 % unspezifisch hemmende monoklonale Anti-alpha-Amylase-Antikörper, ein Verfahren zu ihrer Herstellung und sie enthaltende Arzneimittel.
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公开(公告)号：EP0196921A3
公开(公告)日：1987-12-02
申请号：EP86302432
申请日：1986-04-02
发明人： Fritz, Lawrence C.
CPC分类号： C12N9/6486 , A61K38/00 , C12N1/38 , C12Y304/23015
摘要： Expression vectors and methods for effecting the production of recombinant renin, prorenin, and preprorenin are disclosed. Sufficient quantities are thus obtained to generate pharmaceuticals useful in the treatment of disorders mediated by excessive renin levels such as hypertension. Methods to purify renin and prorenin from cell supernatants and to increase mature renin secretion are also disclosed.
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6.发明公开Methods of altering cells to increase production of a desired compound, cells capable of biosynthesis of a desired compound, methods of producing desired compounds and methods of selecting mutant cells exhibiting increased production of a desired compound 失效改变细胞以增加所需化合物的生产方法，能够生产所需化合物的细胞，生产所需化合物的方法和选择突变细胞的方法展现出所需化合物的生产增加
公开(公告)号：EP0176349A3
公开(公告)日：1986-05-28
申请号：EP85306789
申请日：1985-09-24
发明人： Backman, Keith C.
IPC分类号： C12N15/00 , C12N05/00 , C12N09/00 , C12N09/99 , C12P13/22 , C12P13/08 , C12P13/12 , C12R01/19
CPC分类号： C12N15/52 , C12N15/70 , C12P13/222
摘要： Cells are altered to enhance production of a desired compound by circumventing feedback inhibition of an isozyme that catalyzes a reaction in the the biosynthetic pathway of a desired compound by relying on expression of a gene coding for a non-feedback inhibited isozyme. Also disclosed is a method for selecting mutant cells that have a feedback resistant enzyme that catalyzes a chemical reaction in the biosynthesis of both the desired compound and in the biosynthes of a second compound that is essential for cell growth. The selection is accomplished by growing the cells in feedback-inhibiting concentrations of the inhibitor (i.e., the desired compound or one of its metabolic precursors) and under conditions in which catalytic activity of the enzyme is required for cell growth. Mutants capable of cell growth have avoided enzyme inhibition.
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