公开(公告)号：EP3283518A1

公开(公告)日：2018-02-21

申请号：EP16715331.1

申请日：2016-04-11

申请人： Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V.

发明人： FENDEL, Rolf ,  BARTH, Stefan ,  FISCHER, Rainer ,  REIMANN, Andreas ,  MASKUS, Dominika ,  KLOCKENBRING, Torsten

IPC分类号： C07K16/20

CPC分类号： C07K16/205 ,  A61K2039/507 ,  C07K2317/14 ,  C07K2317/21 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/73 ,  C07K2317/76 ,  C07K2317/92 ,  Y02A50/58

摘要： The technology provided herein relates to novel human antibodies against Plasmodium parasites, in particular against the malaria parasite Plasmodium falciparum. The present disclosure pertains to antibodies against apical membrane antigen 1 (AMA-1). These antibodies have high affinity e.g. to Plasmodium falciparum schizonts and merozoites, inhibit the reinvasion of merozoites into erythrocytes and thereby neutralize parasitic multiplication.

公开(公告)号：EP3273991A1

公开(公告)日：2018-01-31

申请号：EP15713853.8

申请日：2015-03-25

申请人： Fraunhofer Gesellschaft zur Förderung der angewandten Forschung E.V. ,  Rheinisch-Westfälisch-Technische Hochschule Aachen

发明人： STEIN, Christoph ,  MLADENOV, Radoslav ,  STOCKMEYER, Bernhard ,  BARTH, Stefan ,  HEIN, Lea Christin ,  FISCHER, Rainer

IPC分类号： A61K39/395 ,  C07K16/28 ,  A61K47/48 ,  A61K39/00

CPC分类号： A61K39/395 ,  A61K47/6809 ,  A61K47/6849 ,  C07K16/28 ,  C07K2317/74 ,  G01N33/566 ,  G01N33/574

摘要： Complexes suitable for targeting and killing a human target cell comprising a first polypeptide with a binding structure for binding the complex to the cellular surface receptor CD89 (e.g. with an anti CD89 antibody) presented on the cell surface of the human target cell and a second polypeptide comprising a toxic effector domain, preferably Pseudomonas exotoxin A); to nucleic acid molecules encoding said complexes, vectors, host cells containing the nucleic acids and methods for preparation and producing such complexes; compositions and methods for using said complexes for the treatment of diseases, in particular of cancer diseases like leukemia.

公开(公告)号：EP2913392A1

公开(公告)日：2015-09-02

申请号：EP13848830.9

申请日：2013-10-22

申请人： Sekisui Chemical Co., Ltd. ,  Fraunhofer Gesellschaft zur Förderung der angewandten Forschung e.V.

发明人： FURUTANI, Masahiro ,  UENISHI, Akihiro ,  IWASA, Koichiro ,  JENNEWEIN, Stefan ,  FISCHER, Rainer

IPC分类号： C12N1/21 ,  C12N15/09 ,  C12P5/00

CPC分类号： C12P5/007 ,  C12N9/0008 ,  C12N9/88 ,  C12Y102/07004 ,  C12Y102/99002 ,  C12Y302/01 ,  C12Y402/03027 ,  Y02E50/343

摘要： To provide a series of techniques capable of producing isoprene from syngas or the like.
Provided is a recombinant cell prepared by introducing a nucleic acid encoding isoprene synthase into a host cell having an isopentenyl diphosphate synthesis ability by a non-mevalonate pathway, wherein the nucleic acid is expressed in the host cell, and the recombinant cell is capable of producing isoprene from at least one C1 compound selected from the group consisting of carbon monoxide, carbon dioxide, formic acid, and methanol. As the host cell, a Clostridium bacterium or a Moorella bacterium is exemplified. Also provided is a method for producing isoprene using the recombinant cell.

摘要翻译： 提供一系列能够从合成气等生产异戊二烯的技术。 本发明提供一种重组细胞，其通过将编码异戊二烯合酶的核酸通过非甲羟戊酸途径导入具有异戊烯基二磷酸合成能力的宿主细胞中来制备，其中所述核酸在宿主细胞中表达，并且所述重组细胞能够产生 来自至少一种选自一氧化碳，二氧化碳，甲酸和甲醇的C1化合物的异戊二烯。 作为宿主细胞，例举了梭菌属细菌或穆尔氏菌属细菌。 还提供了使用重组细胞生产异戊二烯的方法。

公开(公告)号：EP2847334A1

公开(公告)日：2015-03-18

申请号：EP13723041.3

申请日：2013-05-02

申请人： Fraunhofer Gesellschaft zur Förderung der angewandten Forschung e.V.

发明人： MÜLLER, Boje ,  PRÜFER, Dirk ,  FISCHER, Rainer

IPC分类号： C12N15/29 ,  C12N5/10 ,  C12N15/81 ,  C12N15/62 ,  C12N11/00 ,  C07K14/415

摘要： The invention relates to an artificial forisome body which comprises a fusion protein consisting of at least one SEO-F protein or a section thereof that comprises at least 50 amino acids, and at least one additional protein or peptide with the exception of the GFP and Venus proteins, in which (a) said additional protein or peptide has a mass of no more than 30 kDa, and/or (b) the forisome body also comprises a non-fusion SEO-F protein or a form of this protein that is C-terminally deleted by up to 45 amino acids and/or N-terminally deleted by up to 13 amino acids, the non-fusion SEO-F protein being selected from those proteins which have the property of being able to form homomeric forisome bodies in the absence of additional SEO-F proteins, or (c) the additional protein or peptide is a section of a second SEO-F protein provided that one of the two SEO-F proteins, in a non-fusion form, is capable of forming homomeric forisome bodies, and the fusion protein consists of an N-terminal SEO-F protein section and a C-terminal SEO-F protein section, said sections constituting an SEO-F protein that is complete or C-terminally deleted by up to 50 amino acids and/or N-terminally deleted by up to 13 amino acids, provided that, if required, any number of the four cysteines present in the native SEO-F proteins in the C-terminal section between AS 600 and AS 670 may be exchanged for amino acids that are unable to form an S-S bridge bond. The invention also relates to a plant cell or yeast cell comprising a vector that codes for the given fusion protein and, if required, also for the non-fusion SEO-F protein or its deleted form as defined in (b), as well as to a vector that can be multiplied in a yeast cell and that comprises a section which codes for the given fusion protein. The artificial forisome body of the invention is suitable for protein-chemical purposes, in particular for providing immobilised enzymes, antibodies, antigens and such proteins or peptides that can be immobilised, on a substrate, on the basis of an affinity reaction with a substrate-bound biological or biochemically-produced material.

摘要翻译： 人工forisome体包括（a）由forisomes（SEO-F）蛋白或该蛋白质的形式的筛元件闭塞所做的是C末端至多45个氨基酸和/或N-末端至多13个氨基酸缺失缺失 ，其中SEO-F蛋白具有能够形成同聚在没有附加SEO-F蛋白的forisome体的性质，和（b）的融合蛋白由至少一种SEO-F蛋白的或SEO的截面 F蛋白确实包含至少50种氨基酸和至少一种另外的蛋白质或肽。 人工forisome体包括（a）由forisomes（SEO-F）蛋白或该蛋白质的形式的筛元件闭塞所做的是C末端至多45个氨基酸和/或N-末端至多13个氨基酸缺失缺失 ，其中SEO-F蛋白具有能够形成同聚在没有附加SEO-F蛋白的forisome体的性质，和（b）的融合蛋白由至少一种SEO-F蛋白的或SEO的截面 F蛋白确实包含至少50个氨基酸和至少一种另外的蛋白质或肽由......组成生物化学活性或可激活蛋白质或肽的，条件是，如果需要的话，任何数量存在于C.天然SEO-F蛋白的四个半胱氨酸的 AS 600和AS之间的670末端部的更换氨基酸确实是不能形成在SS桥键。 独立权利要求中包括了：（1）植物细胞或酵母细胞，其包含（a）第一向量做为SEO-F蛋白的代码，以及（b）第二向量做融合蛋白码; （2）在酵母细胞，其包含的部分再现的向量做融合蛋白码; 和（3）使用融合蛋白，包括在SEO-F蛋白和另外的蛋白质或肽，用于人造forisome体的制备。

公开(公告)号：EP1749210B1

公开(公告)日：2011-04-13

申请号：EP05753990.0

申请日：2005-05-25

申请人： Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V.

发明人： ACHATZ, Gernot ,  HUHN, Michael ,  FISCHER, Rainer ,  FERREIRA, Fatima ,  LUGER, Elke ,  STÖCKER, Michael ,  BARTH, Stefan ,  KLOCKENBRING, Torsten

IPC分类号： G01N33/50 ,  C12Q1/68

CPC分类号： C12N15/1034 ,  C07K16/16 ,  C07K2317/622

摘要： A method is disclosed permitting the determination of comprehensive immunoglobulin-specific reaction profiles of people with immuno-functional disorders by means of the individual V gene repertory of Ig-expressing B-cells. The method comprises the isolation of B-cells from body fluids, the isolation of individual B-cells and the genetic material thereof, the amplification of nucleic acids, coding for the variable regions of an antibody expressed by a B-cell, the recombinant production of antibodies by expression of the amplified material and the determination of the binding of the recombinant antibody to antigens.