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公开(公告)号：EP0035521A4
公开(公告)日：1982-02-16
申请号：EP80901703
申请日：1981-03-23
发明人： DUFFY PATRICK
IPC分类号： C12Q1/00 , C12Q1/34 , C12Q1/40 , C12Q1/50 , C12Q1/54 , G01N33/58 , C12Q1/26 , C12Q1/32 , C12Q1/42 , C12Q1/48
CPC分类号： G01N33/581 , C12Q1/34 , C12Q1/40 , C12Q1/50 , C12Q1/54
摘要： Method of determining a substrate in a sample which comprises converting the substrate to a product in a first stage of a cyclic reaction sequence and converting the product back to the substrate in a second reaction stage of the cyclic reaction sequence. At least one of the first and second reaction stages is enzyme catalyzed. Simultaneously with either the first reaction stage or the second reaction stage an indicating substrate is convened to an indicating product in a non-reversible indicator reaction. After this the loss of indicating substract or gain of indicating product may be determined. The method is particularly efficacious in enzyme immunoassay determinations which include homogeneous enzyme immunoassay and enzyme linked immunoassay wherein the free or bound enzyme-ligand or enzyme antibody conjugate is to be assayed by the method of the invention.
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42.发明公开
公开(公告)号：EP4424838A2
公开(公告)日：2024-09-04
申请号：EP24174492.9
申请日：2006-11-24
申请人： Housey, Gerard M.
发明人： Housey, Gerard M.
IPC分类号： C12Q1/50
CPC分类号： G01N33/5008 , G01N33/5011 , G01N33/502
摘要： This invention relates to methods of identifying, synthesizing, optimizing and profiling compounds that are inhibitors or activators of proteins, both naturally occurring endogenous proteins as well as certain variant forms of endogenous proteins, and novel methods of identifying such variants. The method accelerates the identification and development of compounds as potential therapeutically effective drugs by simplifying the pharmaceutical discovery and creation process through improvements in hit identification, lead optimization, biological profiling, and rapid elimination of toxic compounds. Implementation results in overall cost, reductions in the drug discovery process resulting from the corresponding increases in efficiency.
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43.发明授权
公开(公告)号：EP1960535B1
公开(公告)日：2024-05-08
申请号：EP06838389.2
申请日：2006-11-24
CPC分类号： G01N33/5008 , G01N33/5011 , G01N33/502
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公开(公告)号：EP2473621B1
公开(公告)日：2019-01-09
申请号：EP10813351.3
申请日：2010-09-01
发明人： YANG, Shiaw-Der
IPC分类号： C12Q1/50 , G01N33/573
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公开(公告)号：EP3199639A4
公开(公告)日：2018-04-18
申请号：EP15844093
申请日：2015-09-18
发明人： UEDA SHIGERU , SAKASEGAWA SHINICHI
IPC分类号： C12Q1/48 , C12M1/34 , C12N9/02 , C12N9/04 , C12N9/12 , C12Q1/00 , C12Q1/26 , C12Q1/50 , C12Q1/54
CPC分类号： C12Q1/485 , C12M1/34 , C12N9/12 , C12Q1/008 , C12Q1/34 , C12Q1/48 , C12Q1/50 , C12Q1/54 , C12Q1/61 , C12Y207/01001 , C12Y207/01011 , C12Y207/0103 , C12Y207/0104 , C12Y207/01147 , C12Y207/02003 , C12Y207/03002
摘要： The present invention provides a measuring method for at least one of a kinase forward reaction substrate, a phosphorylated product thereof, and a precursor thereof, and includes a step of conducting an enzymatic cycling reaction by bringing at least a kinase, a first nucleotide coenzyme of the kinase, and a second nucleotide coenzyme having a different nucleoside moiety from the first nucleotide coenzyme into contact with a sample; a step of detecting a signal corresponding to a change of at least one of the first nucleotide coenzyme and a conversion product thereof, and the second nucleotide coenzyme and a conversion product thereof; and (3) a step of calculating, on the basis of the detected change of the signal, an amount of the kinase forward reaction substrate and/or the phosphorylated product thereof contained in the sample.
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46.发明公开
公开(公告)号：EP2473621A1
公开(公告)日：2012-07-11
申请号：EP10813351.3
申请日：2010-09-01
发明人： YANG, Shiaw-Der
IPC分类号： C12Q1/50 , G01N33/573
CPC分类号： G01N33/5073 , C12Q1/485 , G01N33/574 , G01N2800/52
摘要： The present invention relates to methods and compositions for identifying and detecting lethal cell useful for monitoring disease status and therapy response in various types of cancer patients regardless of the etiological origin of the cancer and uses thereof.
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公开(公告)号：EP0881301A4
公开(公告)日：2004-12-08
申请号：EP96941190
申请日：1996-12-06
发明人： KOGA SHINJI , SAKASEGAWA SHINICHI
摘要： A method for determining ADP contained in a liquid sample by means of an enzymatic reaction, which comprises reacting the sample at 15 to 45 DEG C at least in the presence of glucose, ADP-dependent hexokinase, and oxidized NAD(P), a glucose-6-phosphate anhydrase, and one or more salts releasing ions selected among magnesium, cobalt, and manganese ions and then determining the ADP contained in the sample together with the AMP resulting from the reaction based on the amount of the reduced NAD(P) yielded. This method has advantages in that the limit of determination is high because ADP is determined based on the amount of the reduced NAD(P) yielded, and that since the reduced NAD(P) has a definite molecular extinction coefficient, the found value is highly reliable and uninfluenced by the reducing substances, etc., contained in the sample.
摘要翻译： 一种通过酶反应测定在液体样品中含有ADP的方法，该方法包括至少在存在葡萄糖，ADP依赖性己糖激酶和氧化的NAD（P）的情况下使样品在15至45℃下反应，葡萄糖 -6-磷酸脱氢酶和一种或多种释放从镁，钴和锰离子中选出的离子的盐，然后基于还原的NAD（P）的量来确定样品中包含的ADP与由反应产生的AMP一起， 产生。 该方法的优点在于，由于ADP是通过还原的NAD（P）产生量决定的，所以测定的极限很高，而且由于还原的NAD（P）具有确定的分子消光系数，所以发现值高 可靠且不受样品中所含的还原物质等的影响。
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公开(公告)号：EP0791658A4
公开(公告)日：2000-10-04
申请号：EP95936772
申请日：1995-11-10
申请人： ASAHI CHEMICAL IND
发明人： AOKI RYOJI , UEDA SHIGERU
CPC分类号： G01N33/96 , C12Q1/42 , C12Q1/50 , C12Q1/52 , Y10T436/10 , Y10T436/105831 , Y10T436/106664 , Y10T436/108331
摘要： Disclosed is a stabilized enzyme composition for use in clinical examination, comprising: (a) an enzyme component comprising at least two enzymes selected from the group consisting of alkaline phosphatase, creatine kinase and alanine aminotransferase; (b) a stabilizer component comprising effective stabilizing amounts of an albumin, and at least one saccharide selected from the group consisting of trehalose and sorbitol; and (c) an aqueous medium having dissolved therein the components (a) and (b). The enzyme composition of the present invention is stable for a prolonged period of time not only under non-freeze refrigeration conditions, but also under freezing conditions or under conditions for non-freeze refrigeration after thawing of the frozen composition, as compared to the conventional enzymatic compositions. The enzyme composition of the present invention can be advantageously used for the purpose of checking the precision in measurement, correcting measured values and calibrating the amount and activity of an enzyme, in a clinical examination for measuring the enzymatic activity in a sample, such as serum or the like.
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公开(公告)号：EP1038024A1
公开(公告)日：2000-09-27
申请号：EP98966274.7
申请日：1998-12-09
发明人： NAGEL, Rolf , MISTELE, Jürgen , SCHRÖDER, Norbert
IPC分类号： C12Q1/50
CPC分类号： C12Q1/50
摘要： A stabilised reagent for photometric enzymatic determination of creatine kinase (CK) in biological sample material by forming ATP from creatine phosphate and ADP and detection of the ATP thus formed containing an organic or inorganic sulphur compound, preferably with a molar deficiency in relation to the added CK activator. A corresponding reagent remains stable at 2-8 °C for up to 12 months without incurring any significant functional loss.
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公开(公告)号：EP1013774A2
公开(公告)日：2000-06-28
申请号：EP99124783.4
申请日：1999-12-14
CPC分类号： C12Q1/50
摘要： Flüssigreagenz zum Nachweis von Kreatinkinase-Aktivität, in dem zumindest folgende Substanzen enthalten sind: G6PDH, Hexokinase, Kreatinphosphat, Glukose, ADP, NAD(P) und Thioglycerol, wobei die Substanzen auf zwei separate Reagenzansätze aufgeteilt sind und die Reagenzansätze für den Nachweis miteinander vermischt werden, dadurch gekennzeichnet, daß G6PDH in dem einem Ansatz und Thioglycerol mit mindestens einer weiteren Substanz in dem anderen Ansatz enthalten ist:
摘要翻译： 用于检测肌酸激酶活性的，其中以下物质至少液体试剂包括：G6PDH，己糖激酶，磷酸肌酸，葡萄糖，ADP，NAD（P）和硫甘油，与物质被分为试剂的两个独立批次和试剂的批次用于检测混合在一起 的特征在于，所述在G6PDH一个方法中，与硫代甘油附带在不同的方法的至少一种其它物质：
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