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    SYNTHETIC PROTEIN FOLDING CATALYSIS
    61.
    发明公开
    SYNTHETIC PROTEIN FOLDING CATALYSIS 失效
    KATALYSE合成蛋白质的折叠

    公开(公告)号：EP0920528A4

    公开(公告)日：2001-01-31

    申请号：EP97934321

    申请日：1997-07-09

    申请人： WISCONSIN ALUMNI RES FOUND

    发明人： RAINES RONALD T

    IPC分类号： C12N9/10 ,  A61K31/00 ,  A61K31/095 ,  A61P3/00 ,  A61P3/06 ,  A61P43/00 ,  C07K1/00 ,  C07K1/113 ,  C07K14/00 ,  C12N9/02 ,  C12N9/90 ,  C12N9/96 ,  C12P21/02 ,  C12Q1/02 ,  A61K38/00 ,  C12N9/00 ,  C12P21/06

    CPC分类号： C12Q1/02 ,  C07K1/1133 ,  C12N9/0036 ,  C12N9/90 ,  C12P21/02

    摘要： An analysis of the protein folding characteristics of the eukaryotic protein folding enzyme protein disulfide isomerase (PDI) led to a study of the minimally sufficient motif for catalytic activity of that enzyme as well as the prokaryotic enzyme thioredoxin. Based on such study, a model for this catalytic activity was developed which was used to predict what non-protein catalysts might substitute for this enzymatic activity. Based on this analysis, it was predicted that a small molecular weight dithiol molecule having a pKa of less than about 8.0 and an E°' of more than about-0.25V could catalyze the formation of proper disulfide bonds in a eukaryotic protein. Subsequently, it was verified that such a dithiol, such as the exemplary molecule BMC, is capable of catalyzing the proper formation of disulfide bonds, and the proper folding of proteins, both in vivo and in vitro. This permits a small organic molecule to be substituted for an enzymatic system in protein synthesis.

    PRIMATE EMBRYONIC STEM CELLS
    63.
    发明公开
    PRIMATE EMBRYONIC STEM CELLS 失效
    干细胞灵长类动物胚胎

    公开(公告)号：EP0770125A4

    公开(公告)日：1998-01-14

    申请号：EP96903521

    申请日：1996-01-19

    申请人： WISCONSIN ALUMNI RES FOUND

    发明人： THOMSON JAMES A

    IPC分类号： C12N5/073 ,  C12N5/0735 ,  C12N5/00

    CPC分类号： C12N5/0605 ,  C12N5/0603 ,  C12N5/0606 ,  C12N2502/13 ,  C12N2506/02

    摘要： A purified preparation of primate embryonic stem cells is disclosed. This preparation is characterized by the following cell surface markers: SSEA-1 (-); SSEA-3 (+); SSEA-4 (+); TRA-1-60 (+); TRA-1-81 (+); and alkaline phosphatase (+). In a paricularly advantageous embodiment, the cells of the preparation have normal karyotypes and continue to proliferate in an undifferentiated state after continuous culture for eleven months. The embryonic stem cell lines also retain the ability, throughout the culture, to form trophoblast and to differentiate into all tissues derived from all three embryonic germ layers (endoderm, mesoderm and ectoderm). A method for isolating a primate embryonic stem cell line is also disclosed.