公开(公告)号：EP4361256A3

公开(公告)日：2024-07-31

申请号：EP24162728.0

申请日：2018-01-23

申请人： Stemcell Technologies Canada Inc.

发明人： HIRST, Adam ,  KARDEL, Melanie ,  CHANG, Wing ,  HIATT, Michael ,  JERVIS, Eric ,  HUNTER, Arwen

IPC分类号： C12N5/071 ,  C12N5/02 ,  C12N5/0735 ,  C12N5/074

CPC分类号： C12N5/0606 ,  C12N5/0696 ,  C12N2500/3620130101 ,  C12N2501/11520130101 ,  C12N2501/1520130101 ,  C12N2501/72720130101 ,  C12N2501/99920130101 ,  C12N2533/9020130101

摘要： The present disclosure relates to improved supplements, culture media and methods for enhancing the survival or proliferation of mammalian stem cells. In particular, adding a lipid supplement, such as a lipid-enriched carrier (e.g. a lipid-enriched albumin), to the culture medium may enhance the survival and/or proliferation of the stem cells by at least 5% to 65% as compared to a culture medium that does not contain the lipid supplement.

公开(公告)号：EP3847189B1

公开(公告)日：2024-05-22

申请号：EP19783676.0

申请日：2019-09-07

IPC分类号： C07K14/74 ,  C12N5/0735 ,  C12N5/074

CPC分类号： C12N5/0606 ,  C12N5/0696 ,  C12N2310/2020170501 ,  C12N2501/5020130101 ,  C12N2510/0020130101 ,  C07K14/70539

公开(公告)号：EP2348105B1

公开(公告)日：2018-10-24

申请号：EP10075534.7

申请日：2005-09-26

申请人： Mesoblast, Inc.

发明人： Gronthos, Stan ,  Zannettino, Andrew Christopher William

IPC分类号： C12N5/0775

CPC分类号： C12N5/0606 ,  A61K35/28 ,  A61K38/1858 ,  A61K38/1875 ,  A61K38/191 ,  A61K38/195 ,  A61K38/2006 ,  A61L27/3804 ,  A61L27/3839 ,  A61L27/3886 ,  A61L27/3895 ,  C12N5/0662 ,  C12N5/0663 ,  C12N5/0664 ,  C12N5/0665 ,  C12N5/0666 ,  C12N5/0667 ,  C12N5/0668 ,  C12N2510/00 ,  A61K2300/00

摘要： This invention relates to multipotential expanded mesenchymal precursor cell progeny (MEMPs). The present invention also relates to methods for producing MEMPs and to uses of MEMPs for therapeutic applications.

公开(公告)号：EP3383434A1

公开(公告)日：2018-10-10

申请号：EP16802111.1

申请日：2016-11-30

申请人： Koninklijke Nederlandse Akademie van Wetenschappen

发明人： GEIJSEN, Niels ,  SHANG, Peng ,  D'ASTOLFO, Diego

IPC分类号： A61K47/10 ,  A61K47/18 ,  A61K47/26 ,  A61K47/02 ,  C12N15/87 ,  A61K31/205

CPC分类号： C12N15/87 ,  A61K9/0019 ,  A61K33/14 ,  A61K38/1808 ,  A61K38/1825 ,  A61K38/45 ,  A61K45/06 ,  A61K47/02 ,  A61K47/10 ,  A61K47/183 ,  A61K47/22 ,  A61K47/26 ,  A61K48/0033 ,  C12N5/0606 ,  C12N9/22 ,  C12N15/11 ,  C12N2310/20 ,  C12N2501/727 ,  C12N2800/80

摘要： The invention relates to transduction compounds, buffers and methods for introducing molecules into cells. The invention also relates to methods of treatment, pharmaceutical compositions and other uses of the transduction compounds and buffers. The invention also relates to modified cells obtainable by the transduction compounds, buffers and methods of the invention.

公开(公告)号：EP2718424B1

公开(公告)日：2018-10-03

申请号：EP12723066.2

申请日：2012-05-17

申请人： Lonza Walkersville, Inc.

发明人： NIE, Ying ,  ROWLEY, Jonathan Allen ,  FELLNER, Thomas ,  WALSH, Patrick

IPC分类号： C12N5/0735 ,  C12N5/0775

CPC分类号： C12N5/0662 ,  C12N5/0606 ,  C12N5/0607 ,  C12N5/0663 ,  C12N2500/14

摘要： Formulations and methods are disclosed for the harvesting and subsequent passaging of human pluripotent stem cells without the use of enzymes and/or scraping to dislodge cells from cell culture vessels. The formulations and methods permit the harvesting of cells as large clusters from the surface of various cell culture vessels including multilayer cell culture vessels. Further, the formulations and methods provide high yields of harvested cells for subsequent passaging and high post-harvest cell viability. Pluripotent stem cells passaged with the formulations according to the methods remain undifferentiated and express typical stem cell markers, while, at the same time, they retain the differentiation capability and are able to differentiate into the cells in all three germ layers and generate teratomas, even after numerous rounds of harvesting and passaging. These hPSCs also maintain normal karyo-type after passaged with the formulations for extended period of time.

公开(公告)号：EP3368661A1

公开(公告)日：2018-09-05

申请号：EP16795439.5

申请日：2016-10-26

申请人： Cell Cure Neurosciences Ltd. ,  Hadasit Medical Research Services and Development Ltd.

发明人： REUBINOFF, Benjamin Eithan ,  SINGER, Orna ,  BOHANA-KASHTAN, Osnat ,  WISER, Ofer

IPC分类号： C12N5/079

CPC分类号： C12N5/0621 ,  C12N5/0018 ,  C12N5/0606 ,  C12N2501/15 ,  C12N2501/16 ,  C12N2501/999 ,  C12N2506/02 ,  C12N2506/45 ,  C12N2509/00 ,  C12N2533/52 ,  C12N2533/54 ,  G01N33/53

摘要： A method of generating retinal pigment epithelium cells is disclosed. Cell populations comprising same and uses thereof are also disclosed.

公开(公告)号：EP2155861B1

公开(公告)日：2018-09-05

申请号：EP08742643.3

申请日：2008-04-07

申请人： International Stem Cell Corporation

发明人： REVAZOVA, Elena, S. ,  TUROVETS, Nikolay, A. ,  KUZMICHEV, Leonid, N. ,  JANUS, Jeffrey, D.

IPC分类号： C12N5/0735

CPC分类号： C12N5/0606 ,  A61K35/545 ,  C12N5/0609 ,  C12N2500/02 ,  C12N2500/14 ,  C12N2501/115 ,  C12N2501/235 ,  C12N2502/1323

摘要： Methods are disclosed for generating HLA homozygous parthenogenetic human stem cell (hpSC-Hhom) lines from both HLA homozygous and HLA heterozygous donors. These hpSC-Hhom lines demonstrate typical human embryonic stem cell morphology, expressing appropriate stem cell markers and possessing high levels of alkaline phosphatase and telomerase activity. Additionally, injection of these cell lines into immunodeficient animals leads to teratoma formation. Furthermore, in the case of HLA heterozygous donors, the hpSC-Hhom lines inherit the haplotype from only one of the donor's parents. SNP data analysis suggests that hpSC-Hhom lines derived from HLA heterozygous oocyte donors are homozygous throughout the genome as assessed by single-nucleotide polymorphism (SNP) analysis. The protocol as disclosed minimizes the use of animal-derived components, which makes the stem cells more practical for clinical application.

公开(公告)号：EP3365037A1

公开(公告)日：2018-08-29

申请号：EP16858318.5

申请日：2016-10-21

申请人： Indiana University Research & Technology Corporation

发明人： KOEHLER, Karl, R. ,  LEE, Jiyoon

IPC分类号： A61L27/38 ,  A61L27/60 ,  A61K35/36 ,  C12N5/071 ,  C12N5/074

CPC分类号： C12N5/0698 ,  A61K35/36 ,  A61L27/362 ,  A61L27/3633 ,  A61L27/3641 ,  A61L27/3687 ,  A61L27/3813 ,  A61L27/3834 ,  A61L27/3839 ,  A61L27/3891 ,  A61L27/3895 ,  A61L27/52 ,  A61L27/54 ,  A61L27/56 ,  A61L27/58 ,  A61L27/60 ,  A61L2300/412 ,  A61L2300/604 ,  A61L2300/64 ,  A61L2430/34 ,  A61L2430/40 ,  C12N5/0606 ,  C12N5/0629 ,  C12N2501/155 ,  C12N2501/999 ,  C12N2506/03 ,  C12N2506/1346 ,  C12N2513/00 ,  C12N2533/76 ,  C12Q1/6876 ,  C12Q2600/136

摘要： Provided herein are methods for directing differentiation of human pluripotent stem cells into a three-dimensional multilayered skin composition comprising an epidermal layer, a dermal layer, and a plurality of cells capable of forming a functional hair follicle. Also provided herein are three-dimensional, multilayered engineered skin compositions and methods of using the same for drug screening, for screening compounds for effects on hair growth, and for other applications.

公开(公告)号：EP3349573A1

公开(公告)日：2018-07-25

申请号：EP16778528.6

申请日：2016-09-16

申请人： Regeneron Pharmaceuticals, Inc.

发明人： SCHMAHL, Jennifer ,  FRENDEWEY, David ,  KUNO, Junko ,  SIAO, Chia-Jen ,  DROGUETT, Gustavo ,  BAI, Yu ,  AUERBACH, Wojtek

IPC分类号： A01K67/027 ,  C12N5/0735

CPC分类号： A01K67/0271 ,  A01K2207/12 ,  A01K2207/35 ,  A01K2227/105 ,  A01K2267/02 ,  C12N5/0606 ,  C12Q1/6876 ,  C12Q2600/136 ,  C12Q2600/156 ,  C12Q2600/158 ,  G01N33/5073

摘要： Methods and compositions are provided for generating F0 fertile XY female animals. The methods and compositions involve making XY pluripotent or totipotent animal cells, in vitro cell cultures, or embryos that are capable of producing a fertile female XY animal in an F0 generation. Such cells, embryos, and animals can be made by silencing a region of the Y chromosome. Optionally, the cells can also be cultured in feminizing medium such as a low-osmolality medium and/or can be modified to decrease the level and/or activity of an Sry protein. Methods and compositions are also provided for silencing a region of the Y chromosome in an XY pluripotent or totipotent animal cell, or in vitro cell cultures, embryos, or animals derived therefrom, by maintaining an XY pluripotent or totipotent animal cell in a feminizing medium. Methods and compositions are also provided for maintaining a population of XY pluripotent or totipotent animal cells in a feminizing medium and selecting cells or clones having increased capabilities for producing a fertile female XY animal in an F0 generation. Methods and compositions are also provided for screening for compounds with feminizing activity or for optimizing concentrations of components in feminizing media.

公开(公告)号：EP3344034A1

公开(公告)日：2018-07-11

申请号：EP16766164.4

申请日：2016-09-01

申请人： Regeneron Pharmaceuticals, Inc.

发明人： SIAO, Chia-Jen ,  LEE, Hoi-Ching ,  LI, Zhe ,  LAI, Ka-Man Venus ,  THURSTON, Gavin

IPC分类号： A01K67/027 ,  C12N15/85

CPC分类号： A01K67/0275 ,  A01K67/0271 ,  A01K2207/12 ,  A01K2217/05 ,  A01K2217/052 ,  A01K2227/105 ,  A01K2267/0331 ,  A01K2267/0393 ,  C07K7/08 ,  C07K2319/50 ,  C07K2319/61 ,  C12N5/0606 ,  C12N2015/8572 ,  C12N2830/008 ,  C12N2830/36

摘要： This disclosure provides a rodent model of prostate cancer. The rodents disclosed herein comprise a transgene that provides prostate-specific expression of an oncogenic protein (e.g, an SV40 tumor antigen) under the control of 5′ and 3′ regulatory regions of a mouse probasin gene. The rodents develop progressive forms of prostate tumor that resemble the development of human prostate cancer.