公开(公告)号：EP1095143A2

公开(公告)日：2001-05-02

申请号：EP99921292.1

申请日：1999-05-07

申请人： Stichting Sanquin Bloedvoorziening

发明人： VOORBERG, Johannes, Jacobus ,  VAN DEN BRINK, Edward, Norbert ,  TURENHOUT, Ellen, Anne, Maria

IPC分类号： C12N15/13 ,  C07K16/36 ,  C07K16/42 ,  A61K39/395 ,  A61K38/37 ,  C12Q1/68

CPC分类号： C07K16/36 ,  A61K38/00 ,  C12Q1/6876

摘要： A polynucleotide, comprising a contiguous nucleotide sequence coding for a human antibody with factor VIII specificity, or complementary to a nucleotide sequence coding for a human antibody with factor VIII specificity, or capable of selectively hybridizing under stringent conditions to such nucleotide sequence. Such polynucleotide may be used as a probe or primer for detection of factor VIII inhibitors, or be used for producing a recombinant polypeptide. A polypeptide, comprising a contiguous amino acid sequence corresponding to or mimicking a fragment or derivative of a human antibody with factor VIII specificity capable of specific binding to factor VIII. An antibody, comprising a recombinant human antibody with factor VIII specificity or an anti-idiotypic antibody directed against a human antibody with factor VIII specificity. Pharmaceutical compositions which contain such polypeptide or antibody.

公开(公告)号：EP0756638B1

公开(公告)日：2001-04-04

申请号：EP95916845.1

申请日：1995-04-21

申请人： Stichting Sanquin Bloedvoorziening

发明人： VOORBERG, Johannes, Jacobus ,  VAN MOURIK, Jan, Aart ,  MERTENS, Koenraad

IPC分类号： A61K38/36

CPC分类号： C07K14/745 ,  A61K38/00 ,  C07K14/755 ,  C12Q1/6883 ,  C12Q2600/156 ,  Y10S514/802 ,  Y10S514/822

摘要： This invention relates to the diagnosis of congenital defects in the anticoagulant protein C system. Methods that are disclosed are based on the detection of mutations at the cleavage sites of coagulation factors that are under control of activated protein C (APC). Diagnostic tests include analysis of the APC-cleavage sites of factor V and factor VIII, by using specific primers to amplify selectively from RNA, cDNA derived from RNA or chromosomal DNA, parts of factor V and factor VIII that contain cleavage sites for APC. Methods that monitor the presence of mutations at the cleavage sites for APC and their utility in the diagnosis of thrombo-embolic disease are disclosed. The invention further discloses methods for correcting the defects detected according to the invention, as well as novel therapeutic agents which can be used in the treatment of bleeding disorders, which agents are based on the 'defective' Factor V and Factor VIII proteins leading to the thrombotic disorders described hereinabove.

公开(公告)号：EP1036331A1

公开(公告)日：2000-09-20

申请号：EP98957231.8

申请日：1998-11-25

申请人： Universiteit Maastricht ,  Stichting Sanquin Bloedvoorziening

发明人： HERMENS, Willem, Theodoor ,  ROBERS, Markus ,  HACK, Cornelis, Erik ,  AARDEN, Lucien, Adrianus

IPC分类号： G01N33/552 ,  G01N33/543 ,  G01N33/557

CPC分类号： G01N33/552 ,  G01N33/54373 ,  G01N33/557 ,  Y10S435/808 ,  Y10S435/81 ,  Y10S436/808 ,  Y10T436/24

摘要： An immunoassay, e.g. ELISA, method and kit for determining (preferably quantitatively) an analyte adsorbed at a surface or present in a liquid sample, comprising binding said analyte to a solid phase, attaching a marker to the analyte, and detecting marker attached to said solid-phase. The invention proposes to use a combination of marker and detection means (e.g. an enzyme-substrate combination) which is capable of producing a precipitate on solid phase which carries the marker and to detect the binding of analyte to the solid phase by in-situ determining the change of surface mass of said solid phase due to the formation of said precipitate. Ellipsometry is an example of a technique suitable for determining the change of surface mass of the solid phase, which could be made of a silicon- or chromium-sputtered glass slide. The invention shortens the assay time and/or improves the assay sensitivity, and allows to measure extremely low surface concentrations of analytes of interest.

公开(公告)号：EP0979237A1

公开(公告)日：2000-02-16

申请号：EP98908308.4

申请日：1998-02-23

申请人： Stichting Sanquin Bloedvoorziening

发明人： HIEMSTRA, Harry ,  TER HART, Hendricus, Gerardus, Josephus ,  PRINS-DE NIJS, Ingrid, Margaretha, Maria ,  HOEK, Pieter, Johannes ,  OVER, Jan

IPC分类号： C07K1/34 ,  C07K14/745 ,  C12N9/74 ,  C07K14/755

CPC分类号： B01D61/145 ,  C07K1/34 ,  C07K14/745 ,  C07K14/755 ,  C12N9/6429 ,  C12Y304/21005

摘要： Method for removing viruses, in particular small non-envelopped viruses, from a plasma derived product, such as Prothrombin Complex Concentrate. The method comprises prefiltration to remove contaminating high molecular weight proteins, followed by nanofiltration through a membrane with an average pore size of 15 nm. The method is capable or removing > 5.1 log Canine parvovirus and > 6.0 log Hepatitis A virus.