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202 条记录 (耗时 0.019 秒)

    REVERSIBLE LABELING OF ANTIGENS IN BIOLOGICAL SPECIMENS
    1.
    发明公开
    REVERSIBLE LABELING OF ANTIGENS IN BIOLOGICAL SPECIMENS 审中-公开

    公开(公告)号：EP3336545A1

    公开(公告)日：2018-06-20

    申请号：EP16203689.1

    申请日：2016-12-13

    申请人： Miltenyi Biotec GmbH

    发明人： NÖLLE, Volker ,  PANKRATZ, Jennifer ,  GÜNTHER, Gritt

    IPC分类号： G01N33/563

    CPC分类号： G01N33/582 ,  C07K1/13 ,  G01N33/557 ,  G01N33/58 ,  G01N33/6857

    摘要： The present invention provides a method for reversible labeling an antigen A of a biological specimen comprising said antigen A with a monovalent antigen binding molecule K comprising a binding site B for specifically binding said antigen A, the method comprising the steps a) contacting said biological specimen comprising said antigen A with said antigen binding molecule K, thereby generating a complex of A/K, b) removing the monovalent antigen binding molecule K that is not bound in said complex of A/K, wherein the equilibrium dissociation constant (K D ) for the binding of said antigen A and said monovalent antigen binding molecule K has a value of equal or greater than 0.5E-08 M and wherein the dissociation rate constant (k(off)) of said binding has a value of equal or greater than 1E-03 sec-1. In some embodiments of the invention a multimerization component F for multimerization of monovalent antigen binding molecules (F/K complex) is included in the method, wherein said F/K complex can be disrupted.

    IN SITU-DILUTION METHOD AND SYSTEM FOR MEASURING MOLECULAR AND CHEMICAL INTERACTIONS
    7.
    发明公开
    IN SITU-DILUTION METHOD AND SYSTEM FOR MEASURING MOLECULAR AND CHEMICAL INTERACTIONS 审中-公开
    在用于测量分子和化学相互作用原位稀释法系统

    公开(公告)号：EP2524230A1

    公开(公告)日：2012-11-21

    申请号：EP11733396.3

    申请日：2011-01-13

    申请人： Nomadics, Inc. ,  Strande, Chris

    发明人： STRANDE, Chris ,  QUINN, John, Gerard

    IPC分类号： G01N33/558

    CPC分类号： G01N33/557 ,  B01F5/0647 ,  B01F13/0059 ,  B01L3/50273 ,  B01L3/502738 ,  G01N21/55

    摘要： The present invention relates to a method for testing multiple analyte concentrations within a biosensor system through a single injection of sample. The method involves flowing a fluid sample containing a neat analyte concentration along a flow path in a fluid system and diluting the sample by causing it to merge with a fluid that is free of analyte in a second flow path under laminar flow conditions. The merged fluid stream is directed through a turbulent third flow path of a very low dead volume. The third flow path carries the merged fluid stream to a sensing region where the analyte is exposed to an immobilized ligand. The concentration of analyte can be controlled in this method by adjusting the flow rates of the sample flow and analyte-free fluid flow. A fluidic system for carrying out this method is also disclosed.

    A molecular sensor using temporal discrimination
    8.
    发明公开
    A molecular sensor using temporal discrimination 有权
    Molekularsensor mit zeitlicher Diskriminierung

    公开(公告)号：EP2500728A1

    公开(公告)日：2012-09-19

    申请号：EP11158411.6

    申请日：2011-03-16

    申请人： NXP B.V.

    发明人： Gridelet, Evelyne ,  Suy, Hilco ,  Frederix, Filip

    IPC分类号： G01N33/543

    CPC分类号： G01N33/557 ,  G01N27/127 ,  G01N33/5438

    摘要： A sensor device is disclosed, which depends on discrimination in time between groups of binding events of target particles to nano-electrodes. The target particles may be in the liquid phase or in suspension. The nano-electrodes form part of a sensor arrangement having a plurality of sensors. The sensor device is arranged such that different species of target particles arrive at the nano-electrodes at different times, using techniques such as chromatography or application of a field such as an electric, magnetic, or gravitational field. The particles may be labelled or unlabeled. The invention is particularly suited, but not limited, to sensing bioparticles.

    摘要翻译： 公开了一种传感器装置，其取决于目标颗粒与纳米电极的结合事件组之间的时间差异。 目标颗粒可以是液相或悬浮液。 纳米电极形成具有多个传感器的传感器装置的一部分。 传感器装置布置成使得不同种类的目标颗粒在不同时间到达纳米电极，使用诸如色谱法的技术或诸如电，磁场或重力场的场的应用。 颗粒可以被标记或未标记。 本发明特别适用于但不限于感测生物颗粒。

    VELOCITY FACTOR
    9.
    发明公开
    VELOCITY FACTOR 有权
    速度系数

    公开(公告)号：EP2470563A1

    公开(公告)日：2012-07-04

    申请号：EP10747844.8

    申请日：2010-08-19

    申请人： F.Hoffmann-La Roche AG

    发明人： SCHRAEML, Michael ,  VON PROFF, Leopold

    IPC分类号： C07K16/00 ,  A61K39/395

    CPC分类号： G01N33/557 ,  C07K16/00 ,  C07K16/461 ,  C07K2317/24 ,  C07K2317/90 ,  C07K2317/92 ,  C07K2317/94 ,  G01N33/6854

    摘要： The current invention is directed to the velocity factor. Based on the velocity factor antibodies can be classified, i.e. antibodies can be characterized on their binding properties as e.g. entropic or enthalpic antigen binder. A velocity factor based classification does not require detailed thermodynamic determinations and/or calculations. The velocity factor is the ratio of the antigen-antibody complex association rate constants ka determined at 37 °C and 13 °C. As only two experimental determinations are required to calculate the velocity factor this is a fast and high-throughput suited method.