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    Immunoassay and test kits therefor
    63.
    发明公开
    Immunoassay and test kits therefor 失效
    免疫测试仪及其测试仪器

    公开(公告)号：EP0368674A3

    公开(公告)日：1991-10-09

    申请号：EP89311660.8

    申请日：1989-11-10

    申请人： SANYO CHEMICAL INDUSTRIES, Ltd.

    发明人： Sakata, Kazuto ,  Kikutake, Jun-ichiro ,  Sigiura, Masakazu

    IPC分类号： G01N33/543

    CPC分类号： G01N33/57473 ,  G01N33/54306 ,  G01N33/54326 ,  G01N33/544 ,  G01N33/552 ,  G01N33/57476 ,  G01N33/577 ,  G01N33/58 ,  G01N33/76

    摘要： Immunoassays of improved sensitivity, even when monoclonal antibodies are of poor affinity, are disclosed. An immunoassay method of the invention comprises the steps of:
    (1) reacting a material to be measured, an immobilized monoclonal antibody (1st antibody) recognizing said material, a monoclonal antibody (2nd antibody) recognizing said material and falling within a different class or subclass fro the 1st antibody, and a labelled antibody (3rd antibody) recognizing the class or subclass of the 2nd antibody to obtain an immuno-complex, and (2) detecting or determining the labelling fragment. In another aspect, the invention provides immunoassay method comprising the steps of: (1) reacts a material to be measured, a protein (the 1st binding protein) to bind specifically to said material, an immobilized protein (the 2nd binding protein) comprising the rest of the 1st binding protein, a part of which having been excised away, and a labelled antibody razing all or part of the excised fragment of the 1st bonding protein to obtain an immuno-complex, and (2) detecting or determining the labelling fragment.

    Enzyme immunoassay for antigen and solid phase used therefor
    64.
    发明公开
    Enzyme immunoassay for antigen and solid phase used therefor 失效
    EnzymimmuntestfürAntigene unddafürverwendbare feste Phase。

    公开(公告)号：EP0405578A2

    公开(公告)日：1991-01-02

    申请号：EP90112454.5

    申请日：1990-06-29

    申请人： NIPPON SHOJI KABUSHIKI KAISHA

    发明人： Marui, Yoji ,  Hayashi, Chozo ,  Ito, Shigeki ,  Fujio, Mieko ,  Tanaka, Hiroki ,  Nagasaki, Toshihide ,  Soda, Yasuji ,  Kaneta, Hitoshi

    IPC分类号： G01N33/543 ,  G01N33/58

    CPC分类号： G01N33/57473 ,  G01N33/54306 ,  G01N33/54353 ,  G01N33/57476 ,  G01N33/581 ,  G01N33/6878

    摘要： An enzyme immunoassay for an antigen using a solid phase, which employs (a) a biotinylated antibody in which a biotin derivative is bound to a thiol group of an Fab′ fragment of an antibody, (b) an enzyme-labelled antibody, and (c) a solid phase immobilized with a substance capable of specifically reacting with the above biotin derivative selected from the group consisting of avidin, streptoavidin and a derivative thereof which is bound to the solid phase directly or via a linkage between another biotin derivative and a high molecular weight substance bound to the solid phase, or alternatively employs (a′) a solid phase immobilized with a biotinylated antibody via a substance selected from the group consisting of avidin, streptoavidin and a derivative thereof wherein said substance is bound to the biotin moiety of said biotinylated antibody and is also bound to the solid phase directly or via a linkage between another biotin derivative and a high molecular weight substance bound to the solid phase, and (b′) an enzyme-­labelled antibody, and a solid phase used therefor.

    摘要翻译： 使用固相的抗原的酶免疫测定法，其使用（a）生物素化抗体，其中生物素衍生物与抗体的Fab min片段的硫醇基团结合，（b）酶标记的抗体和（ c）固定有与能够与上述生物素衍生物特异性反应的物质的固相，所述物质选自抗生物素蛋白，链霉亲和素及其衍生物，其直接或通过另一生物素衍生物与高 或者通过选自抗生物素蛋白，链霉亲和素及其衍生物的物质（a min）固定有生物素化抗体的固相，其中所述物质与生物素部分结合， 所述生物素化抗体并且还直接或通过另一种生物素衍生物与结合的高分子量物质之间的连接与固相结合 和（b分钟）酶标记的抗体，以及用于其的固相。

    Immunoassay and test kits therefor
    66.
    发明公开
    Immunoassay and test kits therefor 失效
    免疫测试和免疫测定。

    公开(公告)号：EP0368674A2

    公开(公告)日：1990-05-16

    申请号：EP89311660.8

    申请日：1989-11-10

    申请人： SANYO CHEMICAL INDUSTRIES, Ltd.

    发明人： Sakata, Kazuto ,  Kikutake, Jun-ichiro ,  Sigiura, Masakazu

    IPC分类号： G01N33/543

    CPC分类号： G01N33/57473 ,  G01N33/54306 ,  G01N33/54326 ,  G01N33/544 ,  G01N33/552 ,  G01N33/57476 ,  G01N33/577 ,  G01N33/58 ,  G01N33/76

    摘要： Immunoassays of improved sensitivity, even when monoclonal antibodies are of poor affinity, are disclosed.
    An immunoassay method of the invention comprises the steps of:
    (1) reacting a material to be measured, an immobilized monoclonal antibody (1st antibody) recognizing said material, a monoclonal antibody (2nd antibody) recognizing said material and falling within a different class or subclass fro the 1st antibody, and a labelled antibody (3rd antibody) recognizing the class or subclass of the 2nd antibody to obtain an immuno-complex, and (2) detecting or determining the labelling fragment.
    In another aspect, the invention provides immunoassay method comprising the steps of: (1) reacts a material to be measured, a protein (the 1st binding protein) to bind specifically to said material, an immobilized protein (the 2nd binding protein) comprising the rest of the 1st binding protein, a part of which having been excised away, and a labelled antibody razing all or part of the excised fragment of the 1st bonding protein to obtain an immuno-complex, and (2) detecting or determining the labelling fragment.

    摘要翻译： 公开了即使当单克隆抗体具有差的亲和力时，提高灵敏度的免疫测定。 本发明的免疫测定方法包括以下步骤：（1）使待测材料，识别所述材料的固定化单克隆抗体（第一抗体），识别所述材料并落入不同类别的单克隆抗体（第二抗体）或 识别第一抗体的亚类和识别第二抗体的类别或亚类的标记抗体（第三抗体）以获得免疫复合物，和（2）检测或确定标记片段。 另一方面，本发明提供了免疫测定方法，其包括以下步骤：（1）使被测量的材料与蛋白质（第一结合蛋白质）反应以与所述材料特异性结合，所述蛋白质（第二结合蛋白质）包含 第一结合蛋白的其余部分已被切除，并且标记的抗体暴露第一结合蛋白的全部或部分切除的片段以获得免疫复合物，和（2）检测或确定标记片段 。

    The quantitative analysis of antigen by the enzyme-antibody bridge method
    70.
    发明公开
    The quantitative analysis of antigen by the enzyme-antibody bridge method 失效
    抗菌抗体方法对抗原的定量分析

    公开(公告)号：EP0125893A3

    公开(公告)日：1986-10-15

    申请号：EP84303171

    申请日：1984-05-10

    申请人： SUMITOMO CHEMICAL COMPANY, LIMITED ,  SUMITOMO PHARMACEUTICALS COMPANY, LIMITED

    发明人： Noguchi, Hiroshi ,  Nakanishi, Toru ,  Ogino, Shigeo

    IPC分类号： G01N33/54 ,  G01N33/74

    CPC分类号： G01N33/57484 ,  G01N33/54306 ,  G01N33/57473 ,  G01N33/57476 ,  G01N33/581 ,  G01N33/74 ,  G01N2333/475 ,  G01N2333/575

    摘要： An improved methodforthe quantitative analysis of antigen by an enzyme-antibody bridge method, which comprises binding (1) an insoluble carrier or a coated insoluble carrier with (2) an antibody being capable of recognizing an antigen (first antibody), (3) an antigen to be determined, (4) arr antibody being capable of recognizing an antigen originated from an animal species different from the animal species of the first antibody (second antibody), (5) an anti-immunoglobulin antibody being capable of recognizing an immunoglobulin of the same animal species as that of the second antibody, (6) an anti-enzyme antibody prepared by using the same animal species as that of the second antibody, (7) an enzyme in this order, and then measuring the amount of the enzyme in the resulting enzyme-anti-enzyme antibody complex, and determining the amount of the antigen therefrom. Said improved method gives a high sensitivity and hence can also be used for the measurement of very low concentration of an antigen.

    摘要翻译： 一种通过酶 - 抗体桥法定量分析抗原的改进方法，其包括将（1）不溶性载体或包被的不溶性载体与（2）能够识别抗原（第一抗体）的抗体（3）结合 ）待确定的抗原，（4）能够识别源自与第一抗体的动物物种不同的动物物种的抗原的抗体（第二抗体），（5）能够识别第一抗体的抗免疫球蛋白抗体 与第二抗体相同的动物物种的免疫球蛋白，（6）通过使用与第二抗体相同的动物物种制备的抗酶抗体，（7）依次的酶，然后测量 所得酶 - 抗酶抗体复合物中的酶，并从中测定抗原的量。 所述改进的方法给出高灵敏度，因此也可以用于测量非常低浓度的抗原。