利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

2 条记录 (耗时 0.009 秒)

    Method of determining immune complexes specific to tumor associated antigen
    1.
    发明公开
    Method of determining immune complexes specific to tumor associated antigen 失效
    Verfahren zur Bestimmung von a tumorassoziiertem Antigen spezifischen Immunokomplexen。

    公开(公告)号：EP0100914A1

    公开(公告)日：1984-02-22

    申请号：EP83106962.0

    申请日：1983-07-15

    申请人： OTSUKA PHARMACEUTICAL CO., LTD.

    发明人： Ishii, Masaru ,  Akazawa, Shugo

    IPC分类号： G01N33/543 ,  G01N33/574

    CPC分类号： G01N33/57469 ,  G01N33/564 ,  G01N33/57473 ,  G01N33/57476

    摘要： This invention provides a method of determining an immune complex specific to a tumor associated antigen characterized in that it comprises the steps of:

    (1) reacting a complement-binding, tumor associated antigen-antibody complex present in a body fluid as an immune complex either with an insolubilized anti-complement or with an insolubilized substance specifically bindable to the tumor associated antigen selected from the group consisting of basic fetoprotein (BFP), a-fetoprotein (AFP), tumor associated glycolinkage (TAG), and carcinoembryonic antigen (CEA) or the antibody thereagainst constituting the complex to form an immune complex-insolubilized anti-complement bound product or an immune complex-insolubilized substance bound product.
    (2) reacting the immune complex-insolubilized anti-complement bound product with a predetermined amount of labelled substance specifically bindable to the tumor associated antigen selected from the group consisting of BFP, AFP, TAG and CEA or the antibody thereagainst constituting the bound product, or reacting the immune complex-insolubilized substance bound product with a predetermined amount of labelled anti-complement, anti-antibody or protein A.
    (3) separating the resulting reaction product from the unreacted labelled substance or from the unreacted labelled anti-complement, anti-antibody or protein A. and
    (4) determining the specific activity of the reaction product or the separated unreacted reactant. The method is useful for diagnosis of cancers.

    摘要翻译： 本发明提供了确定对肿瘤相关抗原特异性的免疫复合物的方法，其特征在于其包括以下步骤：（1）使体液中存在的补体结合的肿瘤相关抗原 - 抗体复合物作为免疫复合物 与不溶性抗补体或与特异性结合于选自由基因甲胎蛋白（BFP），α-甲胎蛋白（AFP），肿瘤相关的聚乙二醇（TAG））和癌胚抗原（CEA）组成的组中的肿瘤相关抗原的不溶性物质组成， 或构成复合物的抗体，形成免疫复合物不溶解的抗补体结合产物或免疫复合物不溶物质结合产物，（2）使免疫复合物不溶解的抗补体结合产物与预定量的标记物质 特异性结合于选自BFP，AFP，TAG和CEA或th的肿瘤相关抗原 e抗体构成结合产物，或使免疫复合物不溶物质结合产物与预定量的标记的抗补体，抗体或蛋白质A反应。（3）将所得反应产物与未反应的标记物质或 未反应的标记的抗补体，抗体或蛋白质A，和（4）确定反应产物或分离的未反应反应物的比活性。 该方法可用于诊断癌症。