A SYSTEM AND A METHOD FOR PRODUCTION OF VOLATILE NATURAL COMPOUNDS USING SOLID GROWTH MEDIA

    公开(公告)号:EP4400574A1

    公开(公告)日:2024-07-17

    申请号:EP23151430.8

    申请日:2023-01-12

    摘要: The invention relates to a system and a method for production of volatile natural compounds using microorganisms grown on solid growth media. The system comprises a flask with one open end arranged to receive a solid growth medium and a closure arranged to fit in said open end of the flask, said closure being configured to receive two separated, different liquids. The container allows sealing in order to prevent the produced VOCs from escaping the container. The method comprises at least the following steps:
    a) preparation of the container and a suitable solid growth medium,
    b) growth of a selected microbial strain,
    c) inoculation of the microbial strain onto the solid growth medium,
    d) filling the closure with water and a suitable organic solvent separately,
    e) sealing, and
    f) incubation, wherein VOCs are present in the organic solvent.

    DIGITAL ASSAY METHOD, AND DIGITAL ASSAY KIT
    64.
    发明公开

    公开(公告)号:EP4397974A1

    公开(公告)日:2024-07-10

    申请号:EP22864520.6

    申请日:2022-08-30

    摘要: Provided is a digital assay method for determining the concentration of a substance in a solution, as a method available for making the operation of digital assay simpler and easier, the method comprising the steps of: (1) providing a substrate having a chamber array region in which 10,000 to 10 billion chambers having a volume of 1 attoliter to 100 femtoliters are arranged, wherein the substrate has light permeability and the chambers recess from a surface of the substrate, (2) introducing a solution containing a substance onto the chamber array region of the substrate, (3) covering the chamber array region of the substrate with an adhesive tape comprising a base layer and an adhesive layer, followed by pressure-bonding the adhesive layer of the adhesive tape against the surface of the substrate, thereby discharging excess of the solution in the chamber array region outside the chamber array region and sealing each of the chambers 2 containing the solution liquid-tight to function as reactors, (4) detecting the presence or absence of the substance in each of the reactors based on fluorescence emitted from a fluorescent substance resulting from the presence of the substance, and (5) determining the concentration of the substance in the solution based on the number of reactors in which the presence of the substance is detected.

    PCR DEVICE
    66.
    发明公开
    PCR DEVICE 审中-实审

    公开(公告)号:EP4397741A1

    公开(公告)日:2024-07-10

    申请号:EP22864453.0

    申请日:2022-08-29

    发明人: TAJIMA, Hideji

    IPC分类号: C12M1/00 C12M1/34 C12M1/38

    CPC分类号: C12M1/38 C12M1/34 C12M1/00

    摘要: A PCR device 100 includes: a vessel 10 configured to house a reaction solution to be subjected to PCR; a heater-cooler 30 configured to heat and cool the reaction solution in the vessel 10; a cap 20 configured to seal the vessel; and a detection end portion 40 configured to be movable relative to the vessel, in a state of being mounted with the cap 20.