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公开(公告)号：EP2078095B1
公开(公告)日：2013-04-03
申请号：EP07800165.8
申请日：2007-09-04
发明人： DANZER, Martin , POLIN, Helene , HOFER, Katja , FIEDLER, Brigitte , RADLER, Juliane , ROSENHAMMER, Katrin , ATZMÜLLER, Sabine , GABRIEL, Christian
IPC分类号： C12Q1/68
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公开(公告)号：EP2078095A2
公开(公告)日：2009-07-15
申请号：EP07800165.8
申请日：2007-09-04
发明人： DANZER, Martin , POLIN, Helene , HOFER, Katja , FIEDLER, Brigitte , RADLER, Juliane , ROSENHAMMER, Katrin , ATZMÜLLER, Sabine , GABRIEL, Christian
IPC分类号： C12Q1/68
摘要： The invention relates to a method for detecting bacterial contaminations preferably in physiological samples as well as sequences of synthetic oligonucleotides used therefor. Said method comprises the steps of i) extracting the nucleic acid, particularly bacterial DNA, ii) amplification by means of primers and detection by means of oligonucleotides, particularly fluorescence-marked oligonucleotides as hybridization probes, containing a sequence that is selected from among a group encompassing SEQ ID NO:5 to SEQ ID NO:35, preferably in real-time PCR, and iii) evaluation by means of fusion curve analysis.
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公开(公告)号：EP2078095B9
公开(公告)日：2013-07-24
申请号：EP07800165.8
申请日：2007-09-04
发明人： DANZER, Martin , POLIN, Helene , HOFER, Katja , FIEDLER, Brigitte , RADLER, Juliane , ROSENHAMMER, Katrin , ATZMÜLLER, Sabine , GABRIEL, Christian
IPC分类号： C12Q1/68
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