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公开(公告)号：EP3368694A1
公开(公告)日：2018-09-05
申请号：EP16801387.8
申请日：2016-10-28
CPC分类号： C12N15/86 , C07K14/025 , C12N2710/00023 , C12N2710/14143 , C12N2710/22022 , C12N2710/22023 , C12N2710/22042 , C12N2810/40 , C12Q1/6806 , C12Q1/686 , C12Q1/708 , C12Q2545/101
摘要： A method of using a virus-like particle from a polyomavirus having a first nucleic acid as a cargo to detect a second nucleic acid. The method includes providing the virus-like particle from the polyomavirus having the first nucleic acid as the cargo, and detecting, via the virus-like particle from the polyomavirus, the second nucleic acid.
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2.发明公开Verfahren zur Bestimmung des DNA-Methylierungsgrades 审中-公开Verfahren zur Bestimmund des DNA-Methylierungsgrades
公开(公告)号：EP2746403A1
公开(公告)日：2014-06-25
申请号：EP12199152.5
申请日：2012-12-21
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , C12Q2563/131 , C12Q2600/154
摘要： Verfahren zur Bestimmung des normierten DNA-Methylierungsgrads eines DNA-Abschnitts, der nur an einem Genlocus vorkommt, in einer Probe genomischer DNA umfassend die Schritte:
a) Quantitative Bestimmung der Gegenwart des DNA-Abschnitts in der Probe;
b) Quantitative Bestimmung des Vorliegens von zumindest einem differentiell methylierten C eines CpG Dinukleotids innerhalb des DNA-Abschnitts und
c) Bestimmung des normierten DNA-Methylierungsgrads des DNA-Abschnitts über die in den Schritten a) und b) bestimmten Werte.摘要翻译： 确定仅在基因组DNA样品的一个基因座处发生的DNA片段的标准DNA甲基化程度包括（a）定量测定样品中DNA片段的存在，（b）定量测定在 在DNA片段内的胞苷 - 胍二核苷酸（CpG）的至少一个差异甲基化的胞苷，和（c）测定DNA片段的标准DNA甲基化程度超过步骤（a）和（b）中确定的值。 包括用于确定相对DNA甲基化程度的独立权利要求，其包括（a1）第一基因组DNA和第二基因组DNA的甲基化程度的测定，以及通过第一和第二基因组DNA的比例特异性甲基化度测定相对DNA甲基化度 第二个DNA。
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3.发明授权HUMAN TRYPSINOGEN WITH REDUCED AUTOACTIVATION AND ITS USE IN AN IMMUNOASSAY 有权减少自体激活的人胰蛋白酶原及其在免疫测定中的应用
公开(公告)号：EP3052623B1
公开(公告)日：2018-02-21
申请号：EP14781104.6
申请日：2014-09-25
发明人： BÜTTNER, Karin , PRASSE, Agneta , ZÜCHNER, Thole
IPC分类号： C12N9/76 , C12Q1/37 , G01N33/542 , G01N33/58
CPC分类号： C12N9/6427 , C12Q1/37 , C12Y304/21004 , G01N33/542
摘要： The present invention relates to a polypeptide consisting of or comprising a variant of human trypsinogen-1, wherein the variant is characterized in that an amino acid residue selected from A16, A17, K64, K138, N139, L142 and L147 is replaced by an amino acid residue with a negatively charged side chain and/or H18 is replaced by a glutamine or asparagine residue and/or L107 is replaced by an amino acid residue with a positively charged side chain and/or N139 is replaced by an amino acid residue with an aromatic side chain, wherein the variant is cleavable into a polypeptide having a native-like enzymatic activity when compared to human trypsin-1. The present invention further relates to means and method for manufacturing the polypeptide as well as the use of the polypeptide, particularly in an immunoassay.
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