公开(公告)号：EP3052623B1

公开(公告)日：2018-02-21

申请号：EP14781104.6

申请日：2014-09-25

申请人： Life Science Inkubator GmbH Bonn

发明人： BÜTTNER, Karin ,  PRASSE, Agneta ,  ZÜCHNER, Thole

IPC分类号： C12N9/76 ,  C12Q1/37 ,  G01N33/542 ,  G01N33/58

CPC分类号： C12N9/6427 ,  C12Q1/37 ,  C12Y304/21004 ,  G01N33/542

摘要： The present invention relates to a polypeptide consisting of or comprising a variant of human trypsinogen-1, wherein the variant is characterized in that an amino acid residue selected from A16, A17, K64, K138, N139, L142 and L147 is replaced by an amino acid residue with a negatively charged side chain and/or H18 is replaced by a glutamine or asparagine residue and/or L107 is replaced by an amino acid residue with a positively charged side chain and/or N139 is replaced by an amino acid residue with an aromatic side chain, wherein the variant is cleavable into a polypeptide having a native-like enzymatic activity when compared to human trypsin-1. The present invention further relates to means and method for manufacturing the polypeptide as well as the use of the polypeptide, particularly in an immunoassay.