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11 条记录 (耗时 0.015 秒)

    ASSAY OF PEROXIDASE ACTIVITY
    3.
    发明公开
    ASSAY OF PEROXIDASE ACTIVITY 失效
    比色法检测过氧化物酶

    公开(公告)号：EP0898620A1

    公开(公告)日：1999-03-03

    申请号：EP97921240.0

    申请日：1997-04-15

    申请人： PIERCE CHEMICAL COMPANY

    发明人： DAVIS, Paul, D. ,  FEATHER-HENIGAN, Kelli ,  HINES, Kimberly

    IPC分类号： G01N33 ,  C12Q1 ,  G01N21

    CPC分类号： C12Q1/28 ,  G01N33/533

    摘要： Chemiluminescent detection of molecules of synthetic or natural origin such as proteins and nucleic acids (DNA and RNA), as well as other biologic molecules, is increasingly replacing radioactive detection as the method of choice where sensitivity is critical. In such assays, luminescence is customarily achieved by the oxidation of a luminol or isoluminol substrate in the presence of an oxidizing agent such as hydrogen peroxide or hydrogen peroxide source, such as perborate, and a peroxidase catalyst such as horseradish peroxidase. To obtain useful levels of luminescence (e.g., detectable levels) by customary techniques, a luminescent enhancer is also present during oxidation. It has been found in the practice of the present invention that azine enhancers have contained an impurity which reduces the properties of the chemiluminescent assay working solutions. The present invention describes a working solution, a process of using the working solution, and kits containing solutions which can be mixed to form the working solutions comprising a solution which is useful for the chemiluminescent assay of peroxidase activity comprising: a) at least one chemiluminescent cyclic diacylhydrazide, b) at least one azine enhancer, and c) at least one oxidizing agent wherein said azine enhancer comprises less than 0.005 parts mole/mole total basis of azine compounds having a hydrogen atom bonded to a nitrogen atom of the azine ring. It has generally been found that this hydrogen containing azine is a poisoning agent for the performance of the azine enhancer.

    SULFO-N-HYDROXY SUCCINIMIDE AND METHOD OF PREPARATION
    5.
    发明公开
    SULFO-N-HYDROXY SUCCINIMIDE AND METHOD OF PREPARATION 有权
    磺化-N-羟基和方法及其生产

    公开(公告)号：EP1015428A1

    公开(公告)日：2000-07-05

    申请号：EP98946097.7

    申请日：1998-09-17

    申请人： PIERCE CHEMICAL COMPANY

    发明人： BREMMER, Martin, Lee ,  WILKES, Marty, Carey

    IPC分类号： C07D207/416

    CPC分类号： C07C303/22 ,  C07C309/17 ,  C07D207/416

    摘要： The salt of a sulfonated succinic acid is cyclized, then converted to novel sulfonated hydroxamic acids by reaction with hydroxylamine (which is added or formed in situ), and the novel hydroxamic acid is then cyclized to the sulfo-N-hydroxysuccinimide salt. Novel sulfo-hydroxamic acid intermediates are formed during this procedure. A process of forming a sulfo-N-hydroxysuccinimide by cyclizing a sulfohydroxamic acid is also described. Alternatively, the present invention describes a novel method for the synthesis of sulfo-N-hydroxysuccinimide salts in which commercially available sulfosuccinic acid salt in an aqueous medium is heated in the presence of an alcohol to form a diester of the succinic acid sulfonate. The diester is then reacted with hydroxylamine (e.g., from the hydrochloride salt) (e.g., in an alkaline buffered aqueous environment, e.g., at room temperature) to form the sulfo-N-hydroxysuccinimide. This is an extremely simple and direct process that does not produce a significant level of irremovable impurities.

    ASSAY OF PEROXIDASE ACTIVITY
    6.
    发明授权
    ASSAY OF PEROXIDASE ACTIVITY 失效
    比色法检测过氧化物酶

    公开(公告)号：EP0898620B1

    公开(公告)日：2002-11-06

    申请号：EP97921240.4

    申请日：1997-04-15

    申请人： PIERCE CHEMICAL COMPANY

    发明人： DAVIS, Paul, D. ,  FEATHER-HENIGAN, Kelli ,  HINES, Kimberly

    IPC分类号： C12Q1/28 ,  C12Q1/66 ,  G01N33/53 ,  G01N33/58 ,  C12Q1/68

    CPC分类号： C12Q1/28 ,  G01N33/533

    摘要： Chemiluminescent detection of molecules of synthetic or natural origin such as proteins and nucleic acids (DNA and RNA), as well as other biologic molecules, is increasingly replacing radioactive detection as the method of choice where sensitivity is critical. In such assays, luminescence is customarily achieved by the oxidation of a luminol or isoluminol substrate in the presence of an oxidizing agent such as hydrogen peroxide or hydrogen peroxide source, such as perborate, and a peroxidase catalyst such as horseradish peroxidase. To obtain useful levels of luminescence (e.g., detectable levels) by customary techniques, a luminescent enhancer is also present during oxidation. It has been found in the practice of the present invention that azine enhancers have contained an impurity which reduces the properties of the chemiluminescent assay working solutions. The present invention describes a working solution, a process of using the working solution, and kits containing solutions which can be mixed to form the working solutions comprising a solution which is useful for the chemiluminescent assay of peroxidase activity comprising: a) at least one chemiluminescent cyclic diacylhydrazide, b) at least one azine enhancer, and c) at least one oxidizing agent wherein said azine enhancer comprises less than 0.005 parts mole/mole total basis of azine compounds having a hydrogen atom bonded to a nitrogen atom of the azine ring. It has generally been found that this hydrogen containing azine is a poisoning agent for the performance of the azine enhancer.

    Novel 10, 10'-substituted-9,9'-biacridine luminescent molecules and their preparation
    9.
    发明公开
    Novel 10, 10'-substituted-9,9'-biacridine luminescent molecules and their preparation 审中-公开
    新10,10'-取代-9,9'- biacridin发光分子以及它们的制备

    公开(公告)号：EP1026156A2

    公开(公告)日：2000-08-09

    申请号：EP00300767.1

    申请日：2000-02-01

    申请人： PIERCE CHEMICAL COMPANY

    发明人： Katsilometes, George C. ,  Bremmer, Martin L. ,  Hermanson, Greg T. ,  Stack, Jeffrey G. ,  Feather-Henigan, Kelli D. ,  Hines, Kimberley

    IPC分类号： C07D219/02 ,  C07D219/04 ,  C07D219/06 ,  G01N33/58 ,  G01N33/533

    CPC分类号： C07D219/04 ,  C07D219/02 ,  C07D219/06 ,  G01N33/533 ,  G01N33/582

    摘要： Novel symmetrical and asymmetrical 10,10'-substituted-9,9'-biacridines and the synthesis of such symmetrical and asymmetrical 10,10'-substituted-9,9'-biacridine molecules and their derivatives is disclosed. These molecules are shown to produce light by chemiluminescence in the presence of signal solutions. These symmetrical or asymmetrical 10,10'-substituted-9,9'-biacridines are used alone or attached to haptens or macromolecules and are utilized as labels in the preparation of chemiluminescent, homogeneous or heterogeneous assays. They are also used in conjunction with other chemiluminescent label molecules to produce multiple analyte chemiluminescent assays.

    摘要翻译： 新颖对称和不对称10,10- substituiertem -9,9'- biacridines和对称和不对称寻求10,10- substituiertem -9,9'- biacridine分子的合成和它们的衍生物是游离缺失盘。 这些分子被示出通过化学发光信号溶液存在下以产生光。 这些对称或不对称10,10- substituiertem -9,9'- biacridines单独使用或附连到半抗原或大分子和被用作在化学发光，均相或非均相测定法的制备标签。 因此，将在与其他化学发光标记分子结合使用，以产生多种分析物的化学发光测定。

    DEVICE FOR DIALYZING SAMPLES
    10.
    发明授权
    DEVICE FOR DIALYZING SAMPLES 失效
    DEVICE FOR透析样品

    公开(公告)号：EP0720508B1

    公开(公告)日：2000-04-05

    申请号：EP94929274.2

    申请日：1994-09-21

    申请人： PIERCE CHEMICAL COMPANY

    发明人： CLARK, Carl

    IPC分类号： B01D61/28 ,  B01D61/30

    CPC分类号： G01N1/34 ,  B01D61/28 ,  B01D61/30 ,  B01D63/082 ,  G01N2001/4016

    摘要： A dialysis device (10) embodies a hermetically sealed sample chamber formed by a gasket (12) with dialysis membranes (14, 16) affixed to each side in substantially parallel relationship. The gasket (12) is impermeable to the sample being dialyzed, but is penetrable and reusable such that a needle can be inserted through the gasket (12) into the thickness and then withdrawn without sample being permitted to leak. The gasket (12) is of sufficient thickness to accommodate insertion of a needle. The device (10) is fitted into a rigid housing (20) containing windows (24, 26) exposing the dialysis membranes (14, 16) and a channel (34) parallel to the dialysis membranes (14, 16) for directing a needle into the gasket (12) in a direction substantially perpendicular to the edge of the gasket (12) so that the needle can access the chamber without contacting the surface of the membranes (14, 16).