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    Stabilized Nucleic Acid Dark Quencher-Fluorophore Probes
    1.
    发明授权
    Stabilized Nucleic Acid Dark Quencher-Fluorophore Probes 有权
    稳定的核酸暗淬灭剂 - 荧光探针

    公开(公告)号：EP2757091B1

    公开(公告)日：2017-06-28

    申请号：EP14152335.7

    申请日：2009-04-01

    申请人： Biosearch Technologies, Inc.

    发明人： Cook, Ronald M. ,  Lyttle, Matt

    IPC分类号： C07C245/02 ,  C07H21/00 ,  C07D241/46 ,  C07D219/04 ,  C07H19/073 ,  C09B31/02

    CPC分类号： C12Q1/6876 ,  C07D219/04 ,  C07H19/06 ,  C07H19/073 ,  C09B31/02

    摘要： The present invention provides a new class of solids supports for synthesis of modified oligomers of nucleic acids, and nucleic acid probes that have a format expediently synthesized on the new supports. Exemplary solid supports include at least one quencher bound through a linker to the solid support. Various exemplary embodiments include a moiety that stabilizes a duplex, triplex, or higher order aggregation (e.g. hybridization) of nucleic acids of which the oligomer of the invention is a component. Other components of the solid support include moieties that stabilize aggregations of nucleic acids, e.g. intercalators, minor groove binding moieties, bases modified with a stabilizing moiety (e.g. alkynyl moieties, and fluoroalkyl moieties), and conformational stabilizing moieties, such as those described in commonly owned U.S. Patent Application Publication No. 2007/0059752.

    摘要翻译： 本发明提供了用于合成核酸修饰寡聚体的新一类固体载​​体，以及在新载体上方便合成的形式的核酸探针。 示例性的固体载体包括至少一种通过接头与固体载体结合的猝灭剂。 各种示例性实施方案包括稳定本发明寡聚物作为组分的核酸的双链体，三链体或更高级聚集体（例如杂交）的部分。 固体支持物的其他组分包括稳定核酸聚集的部分，例如， 嵌入剂，小沟结合部分，用稳定部分（例如炔基部分和氟代烷基部分）修饰的碱基和构象稳定部分，例如在共同拥有的美国专利申请公开号2007/0059752中描述的那些。