公开(公告)号：EP2630260B1

公开(公告)日：2015-11-18

申请号：EP11785174.1

申请日：2011-10-21

申请人： Advanced Cell Diagnostics, Inc.

发明人： WU, Xingyong ,  WANG, Huei-Yu Fay ,  SU, Nan ,  WANG, Li-Chong ,  LUO, Yuling

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6841 ,  C12Q1/6818 ,  C12Q1/682 ,  C12Q2563/131 ,  C12Q2537/143 ,  C12Q2537/125

公开(公告)号：EP2630260A1

公开(公告)日：2013-08-28

申请号：EP11785174.1

申请日：2011-10-21

申请人： Advanced Cell Diagnostics, Inc.

发明人： WU, Xingyong ,  WANG, Huei-Yu Fay ,  SU, Nan ,  WANG, Li-Chong ,  LUO, Yuling

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6841 ,  C12Q1/6818 ,  C12Q1/682 ,  C12Q2563/131 ,  C12Q2537/143 ,  C12Q2537/125

摘要： Disclosed is a method for in situ detection of one or more target nucleic acids based on a combination of RNAscope® method and a general ISH signal amplification method. This new method produces high signal intensity and while keeps low background noise of signal amplification. The result can be consistently reproduced and the method can be easily adopted for routine clinic diagnostic use. Further, the invention relates to a kit, comprising the components of RNAscope® assay and a general ISH signal amplification assay, for sensitive detection of one or more target nucleic acids.