公开(公告)号：EP1405665A1

公开(公告)日：2004-04-07

申请号：EP02022037.2

申请日：2002-10-01

申请人： Alarvita Biolife Corporation

发明人： Tsou, Te Chun ,  Kong, Zwe-Ling ,  Chang, Ke Liang ,  Lee, Shing-Mou

IPC分类号： B01J13/04

CPC分类号： B01J13/04 ,  A23K40/30 ,  A23K50/00 ,  A23K50/80 ,  A23L29/065 ,  A23P10/35 ,  A23V2002/00 ,  A61K9/5015 ,  A61K9/5036 ,  A61K9/5089 ,  Y02A40/818 ,  A23V2200/224 ,  A23V2250/1886 ,  A23V2250/511 ,  A23V2250/543

摘要： A particle embedded with chemical substances, especially with physiological activity substances, is disclosed. The particle has (a) the embedded substances, embedded in base materials and ranged from about 0.1 to 70 % by weight based on the total weight of the particle; and (b) the base material, selected from hydrogenated plant oil, hydrogenated animal oil, C8-C64 saturated fatty acid or the derivatives thereof, and ranged from about 30 to 99.9 % by weight based on the total weight of the particle. Also, the present invention discloses a method for producing the particle.

摘要翻译： 一种包括30-99.9重量％的基体材料（20）包括氢化植物油，A粒子（10）氢化动物油，8-64C饱和脂肪酸和/或其衍生物; 和0.1-70％包埋物质（40）重量，嵌在基体材料，是新的。 因此独立claimsoft包括用于制备颗粒，其包含在嵌入的物质和介质溶液制备的基底材料的; 熔化基础材料; 所述基体材料和嵌入的物质混合; 和泵送混合的基体材料和嵌入的物质通过管道的介质溶液。 介质液的温度比基体材料的下由> = 50 [度] C.

公开(公告)号：EP1243656A3

公开(公告)日：2003-05-07

申请号：EP02006472.1

申请日：2002-03-22

申请人： Alarvita Biolife Corporation ,  National Health Research Institutes

发明人： Juang, Jyh-Lyh ,  Lee, Dung-Fang

IPC分类号： C12N15/866 ,  C12N7/01

CPC分类号： C12N15/86 ,  C12N7/00 ,  C12N2710/14121 ,  C12N2710/14143

摘要： Compositions and methods are provided that relate to a recombinant virus-based vector, e.g., a baculovirus-based vector, that allows the expression of an exogenous target protein in non-permissive cells (e.g., non-permissive insect cells or mammalian cells) in the absence of expression of a detectable selection marker. The vector includes a nucleic acid sequence encoding a detectable selection marker which is controlled by a promoter that is active in host cells used to screen for recombinant virus but is silent in the non-permissive cell used for expression of the exogenous target protein. The vector also includes an exogenous nucleic acid sequence encoding a target protein under the control of a promoter that is active in the non-permissive cell. This system allows the selection marker to be expressed during viral plaque screening, but not while the target protein is being produced.

公开(公告)号：EP1243656A2

公开(公告)日：2002-09-25

申请号：EP02006472.1

申请日：2002-03-22

申请人： Alarvita Biolife Corporation ,  National Health Research Institutes

发明人： Juang, Jyh-Lyh ,  Lee, Dung-Fang

IPC分类号： C12N15/866 ,  C12N7/01

CPC分类号： C12N15/86 ,  C12N7/00 ,  C12N2710/14121 ,  C12N2710/14143

摘要： Compositions and methods are provided that relate to a recombinant virus-based vector, e.g., a baculovirus-based vector, that allows the expression of an exogenous target protein in non-permissive cells (e.g., non-permissive insect cells or mammalian cells) in the absence of expression of a detectable selection marker. The vector includes a nucleic acid sequence encoding a detectable selection marker which is controlled by a promoter that is active in host cells used to screen for recombinant virus but is silent in the non-permissive cell used for expression of the exogenous target protein. The vector also includes an exogenous nucleic acid sequence encoding a target protein under the control of a promoter that is active in the non-permissive cell. This system allows the selection marker to be expressed during viral plaque screening, but not while the target protein is being produced.

摘要翻译： 提供的组合物和方法涉及基于重组病毒的载体，例如基于杆状病毒的载体，其允许在非允许细胞（例如，非允许昆虫细胞或哺乳动物细胞）中表达外源靶蛋白 没有表达可检测的选择标记。 载体包括编码可检测选择标记的核酸序列，其由在用于筛选重组病毒的宿主细胞中有活性但在用于表达外源靶蛋白的非允许细胞中沉默的启动子控制。 载体还包括编码在非允许细胞中有活性的启动子控制下的靶蛋白的外源核酸序列。 该系统允许在病毒斑块筛选期间表达选择标记，但是在产生靶蛋白时不能表达。