公开(公告)号：EP3071224A1

公开(公告)日：2016-09-28

申请号：EP14827177.8

申请日：2014-11-22

申请人： Amarna Holding B.V.

发明人： HAAN, DE, Petrus Theodorus

IPC分类号： A61K39/00

CPC分类号： A61K39/0008 ,  A61K2039/53 ,  A61K2039/54 ,  A61K2039/577 ,  C12N7/00 ,  C12N2710/22041 ,  C12N2710/22043 ,  C12N2800/30 ,  Y02A50/401

摘要： The present invention provides a method for restoring immune tolerance in vivo. The invention relates to the use of a recombinant gene encoding auto-antigens or parts thereof for restoring immune tolerance to the auto-antigens in vivo, under transcriptional control of polyomaviral early and late promoters. In a preferred embodiment, the invention relates to the use of recombinant polyomaviral gene delivery vector particles, such as simian virus 40 (SV40) viral vector particles encoding one or multiple auto-antigens or parts thereof under transcriptional control of the SV40 early and late promoter, for restoring immune tolerance to the auto-antigens in vivo. The invention also relates to compositions comprising recombinant genes or polyomaviral vectors and uses thereof as treatment for degenerative or dystrophic diseases.

摘要翻译： 本发明提供了一种恢复体内免疫耐受性的方法。 本发明涉及编码自身抗原或其部分的重组基因在多聚病毒早期和晚期启动子的转录控制下在体内恢复对自身抗原的免疫耐受性的用途。 在优选的实施方案中，本发明涉及在SV40早期和晚期启动子的转录控制下使用重组多瘤病毒基因递送载体颗粒，例如编码一种或多种自身抗原或其部分的猿猴病毒40（SV40）病毒载体颗粒 ，用于在体内恢复对自身抗原的免疫耐受性。 本发明还涉及包含重组基因或多瘤病毒载体的组合物及其作为退行性或营养不良疾病治疗的用途。

公开(公告)号：EP2494057A1

公开(公告)日：2012-09-05

申请号：EP10771724.1

申请日：2010-10-26

申请人： Amarna Holding B.V.

发明人： VRIES, DE, Walter Gerhardus

IPC分类号： C12N15/85 ,  C12N5/10

CPC分类号： C12N15/85 ,  C12N2710/22022 ,  C12N2800/108

摘要： The invention relates to methods for the production of a recombinant protein in a mammalian cell and methods to enhance the production of recombinant proteins in mammalian cells. More in particular, the invention provides a cell for the production of a recombinant protein of interest wherein said cell is permissive to a polyomavirus and wherein said cell comprises the genetic elements A and B wherein A encodes a polyomaviral large T antigen or a functional equivalent thereof and B comprises a gene encoding a protein of interest under the functional control of a polyomaviral origin of replication or a functional equivalent thereof,wherein said cell lacks the capability to express a polyomaviral small T antigen or a functional equivalent thereof as well as the capability to express a polyomavirus capsid protein.

公开(公告)号：EP2421982A1

公开(公告)日：2012-02-29

申请号：EP10716326.3

申请日：2010-04-22

申请人： Amarna Holding B.V.

发明人： VRIES, DE, Walter Gerhardus

IPC分类号： C12N15/86

CPC分类号： C12N7/00 ,  C12N2710/22051 ,  C12N2710/22052

摘要： The present invention relates to improved methods for the production of viral particles, viral vector particles and recombinant proteins. In particular, the invention relates to improved methods for the production of recombinant polyomaviral vector particles and polyomaviral vector production cell lines. More in particular, the invention relates to methods for the production of simian polyomaviral vector particles such as simian virus 40 (SV40) viral vector particles. The invention also relates to compositions comprising viral vectors and uses thereof and viral vector particles to treat genetic disorders, transplant rejection, autoimmune diseases, infectious diseases, allergies or cancer. The invention also relates to methods for the production of recombinant proteins in mammalian cells and methods to enhance the production of recombinant proteins in mammalian cells.

摘要翻译： 本发明涉及用于生产病毒颗粒，病毒载体颗粒和重组蛋白的改进方法。 特别地，本发明涉及用于生产重组多瘤病毒载体颗粒和多瘤病毒载体生产细胞系的改进方法。 更特别地，本发明涉及用于生产猿多瘤病毒载体颗粒如猿病毒40（SV40）病毒载体颗粒的方法。 本发明还涉及包含病毒载体的组合物及其用途和病毒载体颗粒，以治疗遗传疾病，移植排斥反应，自身免疫性疾病，感染性疾病，变态反应或癌症。 本发明还涉及在哺乳动物细胞中生产重组蛋白质的方法和增强哺乳动物细胞中重组蛋白质生产的方法。

公开(公告)号：EP3071224B1

公开(公告)日：2019-01-09

申请号：EP14827177.8

申请日：2014-11-22

申请人： Amarna Holding B.V.

发明人： HAAN, DE, Petrus Theodorus

IPC分类号： A61K39/00

公开(公告)号：EP2421982B1

公开(公告)日：2015-07-01

申请号：EP10716326.3

申请日：2010-04-22

申请人： Amarna Holding B.V.

发明人： VRIES, DE, Walter Gerhardus

IPC分类号： C12N15/86

CPC分类号： C12N7/00 ,  C12N2710/22051 ,  C12N2710/22052