Abstract:
A wide-field microscope includes a stage (50) to hold a specimen having a fluorescent material therein, and a multi-photon excitation light source (10) to produce a substantially parallel beam of excitation light (25) having a single photon energy less than an absorption energy required for single photon excitation of the fluorescent material.
Abstract:
A system and method to produce a hologram of a single plane of a three dimensional object includes an electromagnetic radiation assembly to elicit electromagnetic radiation from a single plane of said object, and an assembly to direct the elicited electromagnetic radiation toward a hologram-forming assembly. The hologram-forming assembly creates a hologram that is recorded by an image capture assembly and then further processed to create maximum resolution images free of an inherent holographic artifact.
Abstract:
A high-speed, non-mechanical multiple wavelength illumination source is provided, which contains a first light source (32), a second light source (33) providing a different wavelength of light, and a controller (31) for controlling electric current to the first and second light sources. The illumination source is particularly useful in an apparatus for quantitative luminescence ratio photometry and/or luminescence ratio imaging, and in methods of luminescence emission ratio photometry and luminescence emission ratio imaging, and offers particular advantages in photomicroscopy and in microscopic imaging.
Abstract:
The inventors have discovered a method to improve image quality in holography and, for the first time, utilize lenses made from birefringent materials to advantageously split an incoming beam of either coherent or incoherent light into two coincident beams with different focal lengths that interfere with one another and thus create holograms free of electro-optical or pixelated devices. This discovery has many advantages over current methods to create holograms in which many components, including multiple lenses, other electro-optical devices, and/or beam paths are necessary to create holograms. The current invention provides a purely optical holographic process which has better performance and holographic simplicity, in addition to being able to miniaturize holographic processes more than is currently possible in state of the art holography systems.
Abstract:
The inventors have discovered a method to improve image quality in holography and, for the first time, utilize lenses made from birefringent materials to advantageously split an incoming beam of either coherent or incoherent light into two coincident beams with different focal lengths that interfere with one another and thus create holograms free of electro-optical or pixelated devices. This discovery has many advantages over current methods to create holograms in which many components, including multiple lenses, other electro-optical devices, and/or beam paths are necessary to create holograms. The current invention provides a purely optical holographic process which has better performance and holographic simplicity, in addition to being able to miniaturize holographic processes more than is currently possible in state of the art holography systems.
Abstract:
A system and method to produce a hologram of a single plane of a three dimensional object includes an electromagnetic radiation assembly to elicit electromagnetic radiation from a single plane of said object, and an assembly to direct the elicited electromagnetic radiation toward a hologram-forming assembly. The hologram-forming assembly creates a hologram that is recorded by an image capture assembly and then further processed to create maximum resolution images free of an inherent holographic artifact.
Abstract:
A high-speed, non-mechanical multiple wavelength illumination source is provided, which contains a first light source (32), a second light source (33) providing a different wavelength of light, and a controller (31) for controlling electric current to the first and second light sources. The illumination source is particularly useful in an apparatus for quantitative luminescence ratio photometry and/or luminescence ratio imaging, and in methods of luminescence emission ratio photometry and luminescence emission ratio imaging, and offers particular advantages in photomicroscopy and in microscopic imaging.