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公开(公告)号：EP2398868A4
公开(公告)日：2012-08-08
申请号：EP10743450
申请日：2010-02-22
发明人： ROBY PHILIPPE , BOSSE ROGER , ARCAND MATHIEU
IPC分类号： G01N33/542 , G01N33/543 , G01N33/58
CPC分类号： G01N33/543 , C40B70/00 , G01N33/542 , G01N33/54306 , G01N33/582
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2.发明授权
公开(公告)号：EP1088233B9
公开(公告)日：2006-05-17
申请号：EP99957096.3
申请日：1999-06-16
发明人： JOLY, Erik , JOHNSON, Carl, H. , PISTON, David, W.
IPC分类号： G01N33/58 , C12Q1/66 , G01N33/50 , C12N15/62 , G01N33/542
CPC分类号： C12N9/0069 , C07K14/195 , C07K14/43595 , C07K2319/09 , C07K2319/72 , C07K2319/80 , C07K2319/90 , C12N15/62 , G01N33/542 , G01N2333/43595
摘要： This invention provides bioluminescence resonance energy transfer (BRET) system that comprises four parts: 1) a bioluminescent protein that has luciferase activity; 2) an acceptor fluorophore that can accept the energy from the bioluminescent protein when they are associated, in the presence of the appropriate substrate; 3) a modulator that influences the proximity or the orientation of the bioluminescent protein and the fluorophore, and 4) an appropriate substrate to activate the luciferase activity of the bioluminescent protein. The components of this system interact to influence the spatial relationship between the bioluminescent protein and the fluorophore, that is demonstrated by the light emission from the system. The modulator can be a single entity, covalently attached to both the bioluminescent protein and the fluorophore, it can be two separate entities, each linked covalently to either the bioluminescent protein or the fluorophore, or an alternative configuration that falls within the scope of the invention. Thissystem can be used in both in vivo or in vitro assays to detect molecular changes in a wide variety of applications, and is amenable to automation. In particular, it is useful for assaying protein interactions, enzyme activities and the concentration of analytes or signaling molecules in cells or in solution.
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3.发明公开PEPTIDES AND PEPTIDOMIMETIC COMPOUNDS AFFECTING THE ACTIVITY OF G-PROTEIN-COUPLED RECEPTORS BY ALTERING RECEPTOR OLIGOMERIZATION 失效肽和肽模拟物分子中的变化IN受体OLIGOMERIZATIONSVERFAHREN G蛋白偶联受体阻碍活性
公开(公告)号：EP0910640A2
公开(公告)日：1999-04-28
申请号：EP97933814.0
申请日：1997-07-01
CPC分类号： C07K7/08 , A61K38/00 , C07K14/705 , C07K14/70571 , C07K14/7158 , C07K14/723
摘要： This invention relates to peptides and peptidomimetic compounds that modulate the function of G-protein-coupled receptors by affecting the ratio of receptor monomer to homo-oligomeric forms. Novel short peptides of a preferred length of up to about 15-20 amino acid residues are modeled on transmembrane domains of G-protein-coupled receptors, whose activities are affected by the formation of oligomers. These novel peptides and peptidomimetic compounds can be used to selectively affect the activity of G-protein-coupling receptors, thereby functioning as potential therapeutic drugs, etc.. A preferred peptide is GIIMGTFTLCWLPFFIVNIV.
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公开(公告)号：EP2398868A1
公开(公告)日：2011-12-28
申请号：EP10743450.8
申请日：2010-02-22
发明人： ROBY, Philippe , BOSSE, Roger , ARCAND, Mathieu
CPC分类号： G01N33/543 , C40B70/00 , G01N33/542 , G01N33/54306 , G01N33/582
摘要： Luminescence assays and compositions for assay of biomolecular interaction and activity and detection of modulators of biomolecular interaction and activity are provided. Technology described herein has utility in a variety of assay formats and types, for example, simultaneous monitoring multiple parameters which affect interaction and activity of biological molecules. Compositions and methods are provided herein which include a first solid-phase support associated with a first specific binding agent and a photosensitizer,; a second solid-phase support associated with a second specific binding agent and a first emission system; and a third solid- phase support associated with a third specific binding agent and a second emission system.
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5.发明公开METHOD OF ASSAYING COMPOUNDS WHICH AFFECT THE ACTIVITY OF G PROTEIN-COUPLED RECEPTORS BASED ON MEASUREMENT OF RECEPTOR OLIGOMERIZATION 失效对受体低聚基于测量的方法的活性偶联受体BEEIFLUSSEN G蛋白化合物的试验
公开(公告)号：EP0939902A2
公开(公告)日：1999-09-08
申请号：EP97928072.0
申请日：1997-06-30
IPC分类号： G01N33
CPC分类号： G01N33/94 , G01N2333/726
摘要： This invention is a new method of assaying compounds that modulate the activity of G protein-coupled receptors based on measurement of changes in the relative proportions of monomeric to multimeric receptor polypeptides. More specifically, techniques are described herein which permit the prediction of the pharmacological efficacy of drug candidates based solely on the ability of the candidate compounds to alter the ratio of receptor monomer to homo-oligomeric forms of the receptor. This method provides a novel means of assaying compounds as potential therapeutic drugs at G protein-coupled receptors which is greatly simplified and more generally applicable than existing methods.
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公开(公告)号：EP2812698A4
公开(公告)日：2015-09-23
申请号：EP13747045
申请日：2013-02-06
发明人： ROBY PHILIPPE , POUNDER BRITTANY , DAHAN SOPHIE
IPC分类号： G01N21/64
CPC分类号： G01N21/6486 , G01N21/6408 , G01N21/6428
摘要： Methods for determining the presence or absence of a plurality of analytes in a sample with dual-acceptor time-resolved fluorescence resonance energy transfer are provided.
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7.发明授权A BIOLUMINESCENCE RESONANCE ENERGY TRANSFER (BRET) SYSTEM WITH BROAD SPECTRAL RESOLUTION BETWEEN DONOR AND ACCEPTOR EMISSION WAVELENGTHS AND ITS USE 有权与BETWEEN供体和受体的发射波长及其应用宽光谱解决生物发光共振能量转移（BRET）系统
公开(公告)号：EP1295121B1
公开(公告)日：2006-03-08
申请号：EP00986922.3
申请日：2000-12-22
发明人： JOLY, Erik
IPC分类号： G01N33/53
CPC分类号： G01N33/542 , G01N33/533 , G01N2333/43595
摘要： The present invention provides a bioluminescence resonance energy transfer (BRET) detection system characterised by a broad spectral resolution between donor and acceptor emission wavelengths. The broad spectral resolution between the emission wavelength of the bioluminescent donor protein and the fluorescent acceptor molecule results in an increased signal-to-base ratio and dynamic range in comparison with a basic BRET system.
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公开(公告)号：EP2398868B1
公开(公告)日：2016-06-15
申请号：EP10743450.8
申请日：2010-02-22
发明人： ROBY, Philippe , BOSSE, Roger , ARCAND, Mathieu
IPC分类号： G01N33/542 , G01N33/58 , G01N33/543
CPC分类号： G01N33/543 , C40B70/00 , G01N33/542 , G01N33/54306 , G01N33/582
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公开(公告)号：EP2812698A1
公开(公告)日：2014-12-17
申请号：EP13747045.6
申请日：2013-02-06
发明人： ROBY, Philippe , POUNDER, Brittany , DAHAN, Sophie
IPC分类号： G01N33/53 , C12Q1/00 , G01N33/573
CPC分类号： G01N21/6486 , G01N21/6408 , G01N21/6428
摘要： Methods for determining the presence or absence of a plurality of analytes in a sample with dual-acceptor time-resolved fluorescence resonance energy transfer are provided.
摘要翻译： 提供了用双受体时间分辨荧光共振能量转移确定样品中是否存在多种分析物的方法。
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