公开(公告)号：EP1232248A1

公开(公告)日：2002-08-21

申请号：EP00965097.9

申请日：2000-09-15

申请人： Chemogen, Inc. ,  Blyden, Eluemuno R. ,  Grabko, Vladimir I.

发明人： GRABKO, Vladimir, I. ,  BLYDEN, Eluemuno, R.

IPC分类号： C12N7/00 ,  C12N5/00 ,  C12N5/02 ,  C07H21/02

CPC分类号： C12N15/86 ,  C12N2710/10243

摘要： We describe a new technology for producing eggs containing recombinant proteins based on the novel use of avian adenoviruses (AAV). Adenovirus infected embryonated eggs are used as the host organism. The proteins synthesized are isolated in high amounts per egg and purified excluding time-consuming steps of purification. As far as is known, the mechanisms of translation and post-translational modification of proteins in avian species fully resembles those of other higher eukaryotes such as humans, veterinary animals, and plants. In contrast with bacterial, yeast and plant host systems for producing recombinant proteins, avian embryos allow isolation of proteins in biologically active form free of endotoxins. The combination of high productivity and possibility of obtaining proteins in native form makes the system a unique choice for biotechnology, much more favorable than other hosts currently being used for this purpose.