公开(公告)号：EP2606139B1

公开(公告)日：2015-07-15

申请号：EP11818555.2

申请日：2011-08-08

申请人： Codexis, Inc. ,  Cabirol, Fabien L. ,  Gohel, Anupam ,  Collier, Steven J. ,  Liang, Jack ,  Mock, Marissa ,  Mundorff, Emily ,  Novick, Scott ,  Limanto, John ,  Smith, Derek ,  Beutner, Gregory ,  Klapars, Artis ,  Ashley, Eric ,  Strotman, Hallena ,  Truppo, Matthew ,  Hughes, Gregory ,  Grau, Brendan ,  Janey, Jacob

发明人： CABIROL, Fabien, L. ,  GOHEL, Anupam ,  COLLIER, Steven, J. ,  LIANG, Jack ,  MOCK, Marissa ,  MUNDORFF, Emily ,  NOVICK, Scott ,  LIMANTO, John ,  SMITH, Derek ,  BEUTNER, Gregory ,  KLAPARS, Artis ,  ASHLEY, Eric ,  STROTMAN, Hallena ,  TRUPPO, Matthew ,  HUGHES, Gregory ,  GRAU, Brendan ,  JANEY, Jacob

IPC分类号： C12P13/00 ,  C12P7/00

CPC分类号： C12P13/001 ,  C12N9/1096

公开(公告)号：EP2606139A4

公开(公告)日：2014-03-26

申请号：EP11818555

申请日：2011-08-08

申请人： CODEXIS INC ,  CABIROL FABIEN L ,  GOHEL ANUPAM ,  COLLIER STEVEN J ,  LIANG JACK ,  MOCK MARISSA ,  MUNDORFF EMILY ,  NOVICK SCOTT ,  LIMANTO JOHN ,  SMITH DEREK ,  BEUTNER GREGORY ,  KLAPARS ARTIS ,  ASHLEY ERIC ,  STROTMAN HALLENA ,  TRUPPO MATTHEW ,  HUGHES GREGORY ,  GRAU BRENDAN ,  JANEY JACOB

发明人： CABIROL FABIEN L ,  GOHEL ANUPAM ,  COLLIER STEVEN J ,  LIANG JACK ,  MOCK MARISSA ,  MUNDORFF EMILY ,  NOVICK SCOTT ,  LIMANTO JOHN ,  SMITH DEREK ,  BEUTNER GREGORY ,  KLAPARS ARTIS ,  ASHLEY ERIC ,  STROTMAN HALLENA ,  TRUPPO MATTHEW ,  HUGHES GREGORY ,  GRAU BRENDAN ,  JANEY JACOB

IPC分类号： C12P13/00 ,  C12P7/00

CPC分类号： C12P13/001 ,  C12N9/1096

公开(公告)号：EP2606139A2

公开(公告)日：2013-06-26

申请号：EP11818555.2

申请日：2011-08-08

申请人： Codexis, Inc. ,  Cabirol, Fabien L. ,  Gohel, Anupam ,  Collier, Steven J. ,  Liang, Jack ,  Mock, Marissa ,  Mundorff, Emily ,  Novick, Scott ,  Limanto, John ,  Smith, Derek ,  Beutner, Gregory ,  Klapars, Artis ,  Ashley, Eric ,  Strotman, Hallena ,  Truppo, Matthew ,  Hughes, Gregory ,  Grau, Brendan ,  Janey, Jacob

发明人： CABIROL, Fabien, L. ,  GOHEL, Anupam ,  COLLIER, Steven, J. ,  LIANG, Jack ,  MOCK, Marissa ,  MUNDORFF, Emily ,  NOVICK, Scott ,  LIMANTO, John ,  SMITH, Derek ,  BEUTNER, Gregory ,  KLAPARS, Artis ,  ASHLEY, Eric ,  STROTMAN, Hallena ,  TRUPPO, Matthew ,  HUGHES, Gregory ,  GRAU, Brendan ,  JANEY, Jacob

IPC分类号： C12P13/00 ,  C12P7/00

CPC分类号： C12P13/001 ,  C12N9/1096

摘要： The disclosure provides transaminase polypeptides capable of converting the substrate, 2-(3,4-dimethoxyphenethoxy)cyclohexanone to the trans diastereomer product (1R,2R)-2-(3,4-dimethoxyphenethoxy)cyclohexanamine in at least a 2:1 diastereomeric ratio relative to the cis diastereomer (1R,2S)-2-(3,4-dimethoxyphenethoxy)cyclohexanamine. The disclosure also provides polynucleotides, vectors, host cells, and methods of making and using the transaminase polypeptides in processes for preparing (1R,2R)-2-(3,4-dimethoxyphenethoxy)cyclohexanamine and its analogs, which can product compounds can be further used to prepare the aminocyclohexylether compound, (3R)-1-[(1R,2R)-2-[2-(3,4-dimethoxyphenyl)ethoxy]cyclohexyl]pyrrolidin-3-ol, which is an ion channel blocker.

摘要翻译： 本公开提供了能够以至少2:1非对映异构体将底物2-（3,4-二甲氧基苯乙氧基）环己酮转化成反式非对映异构体产物（1R，2R）-2-（3,4-二甲氧基苯乙氧基）环己胺的转氨酶多肽 相对于顺式非对映异构体（1R，2S）-2-（3,4-二甲氧基苯乙氧基）环己胺的比例。 本公开还提供了用于制备（1R，2R）-2-（3,4-二甲氧基苯乙氧基）环己胺及其类似物的方法中制备和使用转氨酶多肽的多核苷酸，载体，宿主细胞和方法， 进一步用于制备作为离子通道阻断剂的氨基环己基醚化合物（3R）-1 - [（1R，2R）-2- [2-（3,4-二甲氧基苯基）乙氧基]环己基]吡咯烷-3-醇。