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1.发明授权
公开(公告)号：EP1204421B1
公开(公告)日：2008-11-12
申请号：EP00954739.9
申请日：2000-08-18
IPC分类号： A61K39/00 , A61K39/02 , A61K39/002 , A61K39/385 , A61P31/04 , A61P33/02
CPC分类号： A61K39/385 , A61K2039/52 , A61K2039/6043 , Y02A50/412
摘要： The present invention relates to a method for producing and isolating specific immunogenic heat shock proteins induced by heat or tumour necrosis factor treatment of cells infected by intra-cellular pathogens; and vaccines prepared from such proteins.
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公开(公告)号：EP1204420B1
公开(公告)日：2007-04-18
申请号：EP00954737.3
申请日：2000-08-18
CPC分类号： A61K39/0275 , A61K39/0258 , A61K2039/52 , A61K2039/55511 , Y02A50/474 , Y02A50/482
摘要： The present invention relates to methods for using prokaryotic cells which have been modified or induced to synthesize trehalose as vaccines and to vaccine compositions obtained thereby.
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公开(公告)号：EP1105155B1
公开(公告)日：2004-11-24
申请号：EP99940299.3
申请日：1999-08-19
CPC分类号： A61K39/0011
摘要： The present invention relates to a method for producing and isolating specific immunogenic heat shock proteins from cancer cells or virally infected or recombinant cells and vaccines prepared from such proteins.
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4.发明公开ANTIGENIC FRAGMENTS ASSOCIATED WITH STRESS-INDUCED PROTEINS AND THEIR USE AS VACCINES 审中-公开具有应力诱导蛋白相关抗原片段和它们作为疫苗
公开(公告)号：EP1257817A2
公开(公告)日：2002-11-20
申请号：EP01905968.2
申请日：2001-02-22
CPC分类号： C07K14/35 , A61K39/015 , A61K39/04 , A61K39/385 , A61K2039/6043 , A61K2039/622 , C07K14/195 , C07K14/44
摘要： The present invention relates to a method for isolating and identifying specific immunogenic pathogen-specific peptides associated with stress proteins induced by the treatment of pathogens and pathogen-infected cells with stress inducing stimuli. The invention also relates to the use of the antigenic fragments derived from complexes thereof with heat shock or other stresses proteins as the immunogenic determinant in vaccine compositions.
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公开(公告)号：EP2341926B1
公开(公告)日：2015-07-01
申请号：EP09785592.8
申请日：2009-09-07
发明人： COLACO, Camilo , SALIM, Kamran
IPC分类号： A61K39/00 , A61K39/04 , A61K39/095 , A61K39/385 , C07K14/195 , C07K14/22 , C07K14/35 , C07K14/47 , C07K1/18
CPC分类号： C07K1/18 , A61K39/0011 , A61K39/04 , A61K39/095 , A61K2039/5256 , C07K14/00 , C07K2319/30 , C12N2710/14143 , C12N2760/16122 , C12N2770/24222 , Y02A50/394 , Y02A50/397 , Y02A50/403 , Y02A50/466 , Y02A50/478
摘要： The present invention provides a method for the purification of complexes comprising a stress protein complexed to a peptide or peptide fragment, from a source mixture, typically a cell lysate. The improved method of the invention provides for protein complexes to be purified using ion exchange based methods, without the need to use chemicals such as chaotropes and ampholytes. The purified complexes can be used as the immunogenic determinant in vaccine compositions for the treatment or prevention of infectious diseases or cancerous conditions.
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6.发明公开
公开(公告)号：EP2442825A1
公开(公告)日：2012-04-25
申请号：EP10732406.3
申请日：2010-06-21
IPC分类号： A61K39/00 , A61K39/385 , C07K1/00
CPC分类号： C07K1/18 , A61K39/0011 , A61K39/04 , A61K39/092 , A61K39/095 , A61K39/12 , A61K39/145 , A61K39/29 , A61K39/385 , A61K39/39 , A61K2039/55516 , A61K2039/605 , C07K14/195 , C12N2710/14043 , C12N2760/16134 , C12N2770/24234 , Y02A50/394 , Y02A50/397 , Y02A50/403 , Y02A50/464 , Y02A50/466 , Y02A50/478
摘要： The present invention provides an improved method for the purification of a mixture of complexes comprising a stress protein complexed to a peptide or peptide fragment from a source mixture, typically a cell lysate. The method of the invention provides for protein complexes to be purified using ion exchange chromatography based methods, wherein a modified buffer solution is used which results in the purified stress protein complexes being more immunogenic than protein complexes obtained using conventional methodology. The purified complexes can be used to produce improved vaccine preparations which elicit enhanced immune responses in the subjects to whom the vaccine compositions are administered.
摘要翻译： 本发明提供了一种用于纯化复合物的混合物的改进方法，其包含与源混合物（通常为细胞裂解物）的肽或肽片段复合的应激蛋白。 本发明的方法提供使用基于离子交换层析的方法纯化的蛋白质复合物，其中使用修饰的缓冲溶液，其导致纯化的应激蛋白复合物比使用常规方法获得的蛋白质复合物更具免疫原性。 纯化的复合物可用于产生改进的疫苗制剂，其在施用疫苗组合物的受试者中引起增强的免疫应答。
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公开(公告)号：EP1315518A2
公开(公告)日：2003-06-04
申请号：EP01963230.6
申请日：2001-09-04
CPC分类号： A61K39/04 , A61K2039/523 , A61K2039/6043
摘要： The present invention provides a vaccine comprising a microbial pathogen, wherein the microbial pathogen is subjected to a stress inducing stimuli. The stress inducing stimuli can be heat or osmotic stress, through preferably the microbial pathogen is genetically modified such that at least one repressor gene for a heat shock protein gene is inactived, this allowing the constitutive expression of heat shock proteins. In particular the use of heat shock protein repressor mutant bacteria is shown to be effective for inducing immunity when comprised within vaccines of the present invention. The present invention further provides a method for producing a vaccine comprising stressed induced microbial pathogens and further the use of the heat shock protein repressor deletion mutant microbes as vaccine vectors which can be additionally allow the expression of heterologous antigen fragments.
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8.发明授权METHOD FOR THE PRODUCTION OF PROTEIN COMPLEXES AND VACCINE COMPOSITIONS COMPRISING THE SAME 有权生产蛋白质复合物的方法和包含它们的疫苗组合物
公开(公告)号：EP2688589B1
公开(公告)日：2017-05-17
申请号：EP12710781.1
申请日：2012-03-23
发明人： COLACO, Camilo , McENTEE, Ian
IPC分类号： A61K39/385 , A61K38/16 , A61K39/00
CPC分类号： C12P21/00 , A61K38/1709 , A61K39/0002 , A61K39/0011 , A61K39/04 , A61K39/08 , A61K39/092 , A61K39/095 , Y02A50/403 , Y02A50/478 , Y02A50/48
摘要： The present invention relates to methods for the production of heat shock protein complexes for use in vaccine compositions. In particular, there is provided a method for increasing the level and immunogenicity of heat shock protein complexes produced in cells by subjecting the cells to specific stress inducing stimuli. The invention further extends to the use of heat shock protein complexes produced according to the methods of the invention in the preparation of vaccine compositions for the prevention and treatment of infectious diseases and cancerous conditions.
摘要翻译： 本发明涉及生产用于疫苗组合物的热休克蛋白复合物的方法。 具体而言，提供了通过使细胞经受特定诱导应激的刺激来提高细胞中产生的热休克蛋白复合物的水平和免疫原性的方法。 本发明进一步扩展到根据本发明的方法产生的热休克蛋白复合物在制备用于预防和治疗感染性疾病和癌症的疫苗组合物中的用途。
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9.发明公开METHOD FOR THE PRODUCTION OF PROTEIN COMPLEXES AND VACCINE COMPOSITIONS COMPRISING THE SAME 有权用于生产蛋白复合物和疫苗组合THEREFORE
公开(公告)号：EP2688589A1
公开(公告)日：2014-01-29
申请号：EP12710781.1
申请日：2012-03-23
发明人： COLACO, Camilo , McENTEE, Ian
IPC分类号： A61K39/385 , A61K38/16 , A61K39/00
CPC分类号： C12P21/00 , A61K38/1709 , A61K39/0002 , A61K39/0011 , A61K39/04 , A61K39/08 , A61K39/092 , A61K39/095 , Y02A50/403 , Y02A50/478 , Y02A50/48
摘要： The present invention relates to methods for the production of heat shock protein complexes for use in vaccine compositions. In particular, there is provided a method for increasing the level and immunogenicity of heat shock protein complexes produced in cells by subjecting the cells to specific stress inducing stimuli. The invention further extends to the use of heat shock protein complexes produced according to the methods of the invention in the preparation of vaccine compositions for the prevention and treatment of infectious diseases and cancerous conditions.
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公开(公告)号：EP2341926A1
公开(公告)日：2011-07-13
申请号：EP09785592.8
申请日：2009-09-07
发明人： COLACO, Camilo , SALIM, Kamran
IPC分类号： A61K39/00 , A61K39/04 , A61K39/095 , A61K39/385 , C07K14/195 , C07K14/22 , C07K14/35 , C07K14/47 , C07K1/18
CPC分类号： C07K1/18 , A61K39/0011 , A61K39/04 , A61K39/095 , A61K2039/5256 , C07K14/00 , C07K2319/30 , C12N2710/14143 , C12N2760/16122 , C12N2770/24222 , Y02A50/394 , Y02A50/397 , Y02A50/403 , Y02A50/466 , Y02A50/478
摘要： The present invention provides a method for the purification of complexes comprising a stress protein complexed to a peptide or peptide fragment, from a source mixture, typically a cell lysate. The improved method of the invention provides for protein complexes to be purified using ion exchange based methods, without the need to use chemicals such as chaotropes and ampholytes. The purified complexes can be used as the immunogenic determinant in vaccine compositions for the treatment or prevention of infectious diseases or cancerous conditions.
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