公开(公告)号：EP1223224A1

公开(公告)日：2002-07-17

申请号：EP00963012.0

申请日：2000-09-29

申请人： KIKKOMAN CORPORATION ,  Sakaue, Ryoichi, Kikkoman Corporation ,  Arai, Ayumi, Kikkoman Corporation ,  Kajiyama, Naoki, Kikkoman Corporation ,  Koyama, Yasuji, Kikkoman Corporation

发明人： SAKAUE, Ryoichi ,  ARAI, Ayumi ,  KAJIYAMA, Naoki ,  KOYAMA, Yasuji

IPC分类号： C12Q1/26 ,  C12Q1/37 ,  G01N30/88

CPC分类号： C12Q1/26 ,  C12Q1/37 ,  G01N30/02 ,  Y10S435/975

摘要： Based on a principle that is different to that of a conventional enzymatic method, the present invention provides a novel method for assaying a glycated protein by a simple procedure, within a short period of time, and with high accuracy, and a reagent kit for assaying used in the method. The method for assaying a glycated protein in a sample is realized by treating a glycated protein-containing sample with protease to liberate a glycated peptide, preferably an α-glycated peptide, particularly preferably an α-glycated dipeptide, from a glycated protein, allowing an oxidase to react with the liberated glycated peptide, and determining the produced hydrogen peroxide.

摘要翻译： 基于与常规酶法不同的原理，本发明提供了一种通过简单的方法在短时间内和高精度测定糖化蛋白质的新方法，以及用于测定的试剂盒 用于该方法。 用于测定样品中糖基化蛋白质的方法是通过用蛋白酶处理含糖基化蛋白质的样品来实现的，以从糖基化蛋白质释放糖基化肽，优选α-甘氨酸肽，特别优选α-甘氨酸二肽，允许 氧化酶与释放的糖化肽反应，并测定所产生的过氧化氢。