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    PECTIN LYASE, PECTIN LYASE GENE, ENZYME PREPARATION, AND METHOD FOR PRODUCTION OF SINGLE CELLS OF PLANT TISSUE
    1.
    发明公开
    PECTIN LYASE, PECTIN LYASE GENE, ENZYME PREPARATION, AND METHOD FOR PRODUCTION OF SINGLE CELLS OF PLANT TISSUE 有权
    PECTINLYASE，PECTINLYASEGEN，ENZYMPRÄPARATUND VERFAHREN ZUR HERSTELLUNG VON EINZELZELLEN AUS PFLANZENGEWEBE

    公开(公告)号：EP2492343A1

    公开(公告)日：2012-08-29

    申请号：EP10824704.0

    申请日：2010-07-15

    申请人： Bio-I Co., Ltd ,  Nakano, Yoshihisa

    发明人： NAKANO, Yoshihisa ,  UENOYAMA, Mitsuhiro ,  KOBAYASHI, Erina

    IPC分类号： C12N9/88 ,  C12N5/04 ,  C12N9/98 ,  C12N15/09

    CPC分类号： C12N9/88 ,  A01N63/04 ,  C12P19/00 ,  C12Y402/0201

    摘要： To provide a pectin lyase and a gene for a pectin lyase which can be used in an enzyme preparation for processing plant tissue, and which possesses sufficient maceration activity even under highly acidic conditions.Isolation and purification of a pectin lyase and a gene for a pectin lyase having a molecular weight of 39,500 produced by an Aspergillus filamentous fungus, and possessing a maceration activity and a pectin lyase activity. The pectin lyase has a specified amino acid sequence, an optimal pH of 3.5 for maceration activity, an optimal pH of 4.5 for pectin lyase activity, and deactivates with boiling treatment for 15 minutes at 121°C. Sterilizing heat treatment are unnecessary, because the present invention possesses sufficient maceration activity at pH 3.0-4.0, and the resulting pectin lysate possesses a bactericidal activity and a potent antibacterial activity. It is possible to inexpensively mass produce food products which retain water-soluble components within the cell, because physical treatment such as stirring or beating is also unnecessary.

    摘要翻译： 提供可用于加工植物组织的酶制剂中的果胶裂解酶和果胶裂解酶的基因，即使在高度酸性条件下也具有足够的浸渍活性。果胶裂解酶和果胶基因的分离和纯化 具有分子量为39,500的由曲霉丝状真菌产生的裂解酶，并且具有浸渍活性和果胶裂解酶活性。 果胶裂解酶具有特定的氨基酸序列，浸出活性的最佳pH为3.5，果胶裂解酶活性的最佳pH为4.5，在121℃下沸腾处理15分钟后失活。 灭菌热处理是不必要的，因为本发明在pH3.0-4.0时具有足够的浸渍活性，所得果胶裂解物具有杀菌活性和强力的抗菌活性。 由于不需要诸如搅拌或打浆的物理处理，因此可以廉价地大量生产保留细胞内的水溶性成分的食品。