公开(公告)号：EP3066204A4

公开(公告)日：2017-06-21

申请号：EP14859664

申请日：2014-11-07

申请人： OZGENE HOLDINGS PTY LTD

发明人： KOENTGEN FRANK

IPC分类号： C12N15/877 ,  A01K67/027 ,  C12N5/0735 ,  C12N5/10 ,  C12N15/85

CPC分类号： A01K67/0276 ,  A01K67/0271 ,  A01K2207/12 ,  A01K2217/075 ,  A01K2227/105 ,  A01K2267/025 ,  C12N5/0606 ,  C12N15/8509 ,  C12N2510/00 ,  C12N2800/30

摘要： Methods, compositions and non-human animals and parts thereof are disclosed for improving germ line transmission of genetic modifications. More particular, the present invention discloses methods and compositions for producing non-human embryos with a disrupted or disruptable fertility gene, which can be used as hosts for the development of donor pluripotent cells, including genetically modified donor pluripotent cells, into germ cells and gametes. Also disclosed are methods and compositions for producing from such embryos chimeric non-human animals with a disrupted fertility gene and for breeding the chimeric non-human animals with cognate non-human animals that comprise a fertility gene that lacks a disruption to produce non-human animals having substantially all gametes and/or germ cells derived from the donor pluripotent cells. The present invention also discloses non-human gametes, germ cells, embryos and animals for use in the subject methods.

摘要翻译： 公开了用于改善遗传修饰的种系传递的方法，组合物和非人动物及其部分。 更具体地说，本发明公开了用于产生具有破坏或可破坏的可育性基因的非人类胚胎的方法和组合物，其可用作供体多能细胞（包括遗传修饰的供体多能细胞）发育成生殖细胞和配子的宿主 。 还公开了用于从这些胚胎中产生具有破坏的可育性基因的嵌合非人动物以及用含有同源非人动物的嵌合非人动物进行育种的方法和组合物，所述非人动物包含缺乏产生非人的破坏的生育力基因 具有基本上全部配子和/或生殖细胞来源于供体多能细胞的动物。 本发明还公开了用于本发明方法的非人配子，生殖细胞，胚胎和动物。

公开(公告)号：EP4043575A1

公开(公告)日：2022-08-17

申请号：EP21177140.7

申请日：2014-11-07

申请人： Ozgene Holdings Pty Ltd

发明人： KOENTGEN, Frank

IPC分类号： C12N15/877 ,  C12N5/10 ,  A01K67/027 ,  C12N15/85 ,  C12N5/0735

摘要： Methods, compositions and non-human animals and parts thereof are disclosed for improving germ line transmission of genetic modifications. More particular, the present invention discloses methods and compositions for producing non-human embryos with a disrupted or disruptable fertility gene, which can be used as hosts for the development of donor pluripotent cells, including genetically modified donor pluripotent cells, into germ cells and gametes. Also disclosed are methods and compositions for producing from such embryos chimeric non-human animals with a disrupted fertility gene and for breeding the chimeric non-human animals with cognate non-human animals that comprise a fertility gene that lacks a disruption to produce non-human animals having substantially all gametes and/or germ cells derived from the donor pluripotent cells. The present invention also discloses non-human gametes, germ cells, embryos and animals for use in the subject methods.

公开(公告)号：EP3066204A1

公开(公告)日：2016-09-14

申请号：EP14859664.6

申请日：2014-11-07

申请人： Ozgene Holdings Pty Ltd

发明人： KOENTGEN, Frank

IPC分类号： C12N15/877 ,  C12N5/10 ,  A01K67/027

CPC分类号： A01K67/0276 ,  A01K67/0271 ,  A01K2207/12 ,  A01K2217/075 ,  A01K2227/105 ,  A01K2267/025 ,  C12N5/0606 ,  C12N15/8509 ,  C12N2510/00 ,  C12N2800/30

摘要： Methods, compositions and non-human animals and parts thereof are disclosed for improving germ line transmission of genetic modifications. More particular, the present invention discloses methods and compositions for producing non-human embryos with a disrupted or disruptable fertility gene, which can be used as hosts for the development of donor pluripotent cells, including genetically modified donor pluripotent cells, into germ cells and gametes. Also disclosed are methods and compositions for producing from such embryos chimeric non-human animals with a disrupted fertility gene and for breeding the chimeric non-human animals with cognate non-human animals that comprise a fertility gene that lacks a disruption to produce non-human animals having substantially all gametes and/or germ cells derived from the donor pluripotent cells. The present invention also discloses non-human gametes, germ cells, embryos and animals for use in the subject methods.

摘要翻译： 公开了用于改善遗传修饰的种系传播的方法，组合物和非人动物及其部分。 更具体地，本发明公开了用于产生具有破坏或破坏性生育基因的非人胚胎的方法和组合物，其可以用作供体多能细胞（包括遗传修饰的供体多能细胞）发育成生殖细胞和配子的宿主 。 还公开了用于从具有破坏的生殖力基因的这种胚胎嵌合非人动物中产生的方法和组合物，并且用于与包含缺乏破坏以产生非人类的生育基因的同源非人动物育种嵌合非人动物 具有基本上所有来自供体多能细胞的配子和/或生殖细胞的动物。 本发明还公开了用于本发明方法的非人配子，生殖细胞，胚胎和动物。