公开(公告)号：EP0888046A1

公开(公告)日：1999-01-07

申请号：EP97903809.0

申请日：1997-01-21

申请人： Paik, Kye-Hyung

发明人： Paik, Kye-Hyung

IPC分类号： C12N15 ,  A61K39 ,  C07K14 ,  C12N5 ,  C12N7 ,  C12P21

CPC分类号： C07K14/005 ,  A61K39/00 ,  C12N7/00 ,  C12N15/86 ,  C12N2730/10122 ,  C12N2730/10143 ,  C12N2770/24222 ,  C12N2770/32422 ,  C12N2799/021 ,  C12N2800/108 ,  C12P21/02 ,  Y02A50/464

摘要： A method of producing viral antigens in vitro by infecting animal organ tissue rich in mitochondria with a virus, including human hepatitis B virus (HBV), and culturing the infected tissue in vitro is disclosed. A method of producing proteins in vitro by transfecting mitochondria-rich animal tissue with a recombinant HBV-based vector and culturing the transfected tissue in a dynamic tissue culture system is disclosed.

公开(公告)号：EP0888046A4

公开(公告)日：2001-10-04

申请号：EP97903809

申请日：1997-01-21

申请人： PAIK KYE HYUNG

发明人： PAIK KYE-HYUNG

IPC分类号： C12N15/09 ,  A61K39/00 ,  A61K39/12 ,  A61K39/29 ,  C07K14/02 ,  C07K14/10 ,  C07K14/18 ,  C12N5/07 ,  C12N5/10 ,  C12N7/00 ,  C12N7/02 ,  C12N15/00 ,  C12N15/06 ,  C12N15/86 ,  C12P21/02 ,  A01N1/02 ,  C12N5/06 ,  C12N5/08 ,  C12P21/00

CPC分类号： C07K14/005 ,  A61K39/00 ,  C12N7/00 ,  C12N15/86 ,  C12N2730/10122 ,  C12N2730/10143 ,  C12N2770/24222 ,  C12N2770/32422 ,  C12N2799/021 ,  C12N2800/108 ,  C12P21/02

摘要： A method of producing viral antigens in vitro by infecting animal organ tissue rich in mitochondria with a virus, including human hepatitis B virus (HBV), and culturing the infected tissue in vitro is disclosed. A method of producing proteins in vitro by transfecting mitochondria-rich animal tissue with a recombinant HBV-based vector and culturing the transfected tissue in a dynamic tissue culture system is disclosed.