公开(公告)号：EP1730570A1

公开(公告)日：2006-12-13

申请号：EP05729984.4

申请日：2005-03-30

申请人： Power, Joan F.

发明人： POWER, Joan, F.

IPC分类号： G02B21/06 ,  G01N21/84

CPC分类号： G01N21/8422 ,  G02B21/088

摘要： An apparatus and method allowing an optimized illumination in a light profile microscope by excitation of a sample with an elliptically collimated beam. The beam, which is typically supplied by a laser, is collimated with unequal beam waist radii (and Rayleigh ranges) along major and minor axes orthogonal to a propagation direction, and approximates a plane sheet of illumination. The plane sheet of illumination is aligned with a thinnest width dimension thereof along the optic axis of the microscope objective, and with a center thereof at the object plane of the objective. Excitation light in a test sample is thereby confined to within a narrow thickness of the object plane of the objective lens, which minimizes out-of-focus light in the image. The major axis width of the plane illumination sheet is typically a factor of ten or more greater than the minimum width, allowing a large area of the test sample to be illuminated and imaged. This excitation arrangement optically emulates the operation of micro-toming a thin cross section of a material for analysis, and provides optimum resolution and field in a light profile microscope.

公开(公告)号：EP1730570A4

公开(公告)日：2010-07-21

申请号：EP05729984

申请日：2005-03-30

申请人： POWER JOAN F

发明人： POWER JOAN F

IPC分类号： G02B21/06 ,  G01N21/84 ,  G02B21/08

CPC分类号： G01N21/8422 ,  G02B21/088

公开(公告)号：EP1730570B1

公开(公告)日：2012-08-22

申请号：EP05729984.4

申请日：2005-03-30

申请人： Power, Joan F.

发明人： Power, Joan F.

IPC分类号： G02B21/06 ,  G01N21/84

CPC分类号： G01N21/8422 ,  G02B21/088

摘要： An apparatus and method allowing an optimized illumination in a light profile microscope by excitation of a sample with an elliptically collimated beam. The beam, which is typically supplied by a laser, is collimated with unequal beam waist radii (and Rayleigh ranges) along major and minor axes orthogonal to a propagation direction, and approximates a plane sheet of illumination. The plane sheet of illumination is aligned with a thinnest width dimension thereof along the optic axis of the microscope objective, and with a center thereof at the object plane of the objective. Excitation light in a test sample is thereby confined to within a narrow thickness of the object plane of the objective lens, which minimizes out-of-focus light in the image. The major axis width of the plane illumination sheet is typically a factor of ten or more greater than the minimum width, allowing a large area of the test sample to be illuminated and imaged. This excitation arrangement optically emulates the operation of micro-toming a thin cross section of a material for analysis, and provides optimum resolution and field in a light profile microscope.