摘要:
Disclosed are very high optically pure D- and L- lactic acid fermentation production strains and construction methods thereof and the method for preparing very high optically pure D- and L- lactic acids using the strains, wherein the deposit number of the D- lactic acid fermentation production strain is CGMCC No. 11059, and the deposit number of the L- lactic acid fermentation production strain is CGMCC No. 11060.
摘要:
The present invention discloses a method for porous spinning composite material and lithium extraction application thereof. The method includes the following steps: (1) mixing, heating and stirring a high-molecular polymer and an organic solvent to dissolve fully; and standing and defoaming the mixed solution; (2) adding lithium-ion sieve powder and a water-soluble pore-forming agent into the mixed solution; and fully stirring and uniformly mixing the lithium-ion sieve powder and the water-soluble pore-forming agent; (3) spinning the mixed solution in a coagulating bath by utilizing wet spinning equipment for performing phase inversion molding; and (4) conducting draft washing, and drying and curing the solution to obtain the porous spinning composite adsorption material. The obtained porous spinning composite adsorption material is applied to extract lithium from liquid lithium ore. The present invention effectively solves problems of low fluidity and cycling stability of powder materials. Moreover, relative to granular and rod-shaped adsorption materials, the adsorption rate and adsorption capacity of the porous spinning composite adsorption material are greatly improved. Therefore, the porous spinning composite adsorption material has excellent application prospects in the development and utilization of lithium resources in salt lake brine, underground brine and geothermal water.
摘要:
A transport protein coding gene, and a method for efficient production of L-tryptophan by a strain containing the gene. Specifically, by heterologous expression of ywkB gene from Bacillus subtilis on the genome of Escherichia coli, L-tryptophan production efficiency of the strain can be improved. Performing shake flask fermentation with the strain can accumulate 15.2 g/L of L-tryptophan within 24 h, which is 35% higher than a control strain.
摘要:
The invention belongs to the technical field of enzyme engineering, and particularly relates to lactase for generating galactooligosaccharide as well as preparation and an application of the lactase. According to the invention, lactase (BglD305 derived from Bacillus circulans B2301 and BglD derived from Bacillus circulans ATCC 31382) molecules from two sources are taken as the basis for molecular evolution, so as to obtain new lactase enzyme molecules with high galactooligosaccharide synthesis efficiency and good expression performance; the high-producing strain lactase is further constructed, the lactase can be efficiently synthesized during the submerged fermentation, and the enzyme molecule is secreted into the culture medium, the high-activity enzyme preparation is directly prepared from the fermentation supernatant, and the lactase expression level can achieve 2208 U/mL; the fermentation manufacturing cost of lactase is reduced, the fermentation manufacturing process is simplified, and the quality of the lactase preparation is improved.