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1.发明公开MULTI-TARGETED PRIMING FOR GENOME-WIDE GENE EXPRESSION ASSAYS 有权用于一般性基因表达测定的多目标引物（Multi-TARGET PRIMING for GENEROUS GENE EXPRESSION ASSAYS）
公开(公告)号：EP2566982A4
公开(公告)日：2013-10-16
申请号：EP11778094
申请日：2011-05-02
申请人： TOWNSEND JEFFREY P
发明人： TOWNSEND JEFFREY P
IPC分类号： C12Q1/68
CPC分类号： C12N15/1096 , C12Q1/686 , C12Q1/689 , C12Q1/6895 , C12Q2600/158 , C12Q2600/16 , C12Q1/6809 , C12Q1/6811 , C12Q2521/107 , C12Q2525/179 , C12Q2537/143 , C12Q2537/165
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公开(公告)号：EP2566982B1
公开(公告)日：2015-07-01
申请号：EP11778094.0
申请日：2011-05-02
申请人： Townsend, Jeffrey P.
发明人： Townsend, Jeffrey P.
IPC分类号： C12Q1/68
CPC分类号： C12N15/1096 , C12Q1/686 , C12Q1/689 , C12Q1/6895 , C12Q2600/158 , C12Q2600/16 , C12Q1/6809 , C12Q1/6811 , C12Q2521/107 , C12Q2525/179 , C12Q2537/143 , C12Q2537/165
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公开(公告)号：EP2566982A2
公开(公告)日：2013-03-13
申请号：EP11778094.0
申请日：2011-05-02
申请人： Townsend, Jeffrey P.
发明人： Townsend, Jeffrey P.
IPC分类号： C12Q1/68
CPC分类号： C12N15/1096 , C12Q1/686 , C12Q1/689 , C12Q1/6895 , C12Q2600/158 , C12Q2600/16 , C12Q1/6809 , C12Q1/6811 , C12Q2521/107 , C12Q2525/179 , C12Q2537/143 , C12Q2537/165
摘要： The present invention is a method of generating a library of expressed cDNA sequences using multi-targeted primers that are complementary to degenerate motifs present in a large proportion of corresponding mRNA sequences. Multi-targeting priming (MTP) for genome-wide gene expression assays provides selective targeting of multiple sequences and counter-selection against undesirable sequences. Priming with MTPs in addition to oligo-dT can result in higher sensitivity, a greater number of genes whose expression is well measured, a greater number of genes whose differences in gene expression are detected to be statistically, and a greater power to detect meager differences in expression.
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