公开(公告)号：EP4310183A3

公开(公告)日：2024-02-21

申请号：EP23174169.5

申请日：2018-01-30

申请人： 10X Genomics, Inc.

发明人： BELHOCINE, Kamila ,  BHARADWAJ, Rajiv ,  HINDSON, Christopher ,  SCHNALL-LEVIN, Michael ,  LIN, Bill ,  MAKAREWICZ, Anthony ,  PATEL, Pranav ,  PFEIFFER, Katherine ,  PRICE, Andrew D. ,  RAHIMI LENJI, Mohammad ,  WHEELER, Tobias Daniel ,  YIN, Yifeng

IPC分类号： C12N15/09 ,  C12Q1/68 ,  C12Q1/6806 ,  C12N15/10

摘要： Provided herein are methods and systems for sample preparation techniques that allow amplification, e.g., whole genome amplification, reverse transcription, amplification of cellular nucleic acids, etc., and sequencing of single cells, which may be of interest. The methods and systems generally operate by bringing together a first liquid phase comprising a plurality of biological particles, a second liquid phase comprising gel beads, and a third immiscible phase. The liquid phases may interact to form partitions. The methods and systems may be configured to allow the implementation of a single operation or multi-operation chemical and/or biochemical processing within the partitions. Another aspect of the disclosure provides a composition comprising a cell bead of a plurality of cell beads and a barcode bead of a plurality of barcode beads. Also provided herein are kits for analyzing individual cells or viruses or small populations of cells or viruses.

公开(公告)号：EP4215616A1

公开(公告)日：2023-07-26

申请号：EP22209419.5

申请日：2018-05-17

申请人： 10X Genomics, Inc.

发明人： BHARADWAJ, Rajiv ,  SCHNALL-LEVIN, Michael ,  MAKAREWICZ, Anthony ,  SHORT, Steven

IPC分类号： C12P21/00 ,  C12Q1/02 ,  C12Q1/68 ,  G01N15/14

摘要： Methods and systems for sorting droplets are provided. In some cases, occupied droplets may be sorted from unoccupied droplets. In some cases, singularly occupied droplets may be sorted from unoccupied droplets and multiply occupied droplets. Methods and systems for sorting cell beads are provided. In some cases, cell beads may be sorted from particles unoccupied with cell derivatives. In some cases, singularly occupied cell beads may be sorted from unoccupied particles and multiply occupied cell beads. Methods and systems for selectively polymerizing droplets based on occupancy and size of the droplets are provided.

公开(公告)号：EP4208292A1

公开(公告)日：2023-07-12

申请号：EP21790295.6

申请日：2021-09-02

申请人： 10X Genomics, Inc.

发明人： RAHIMI LENJI, Mohammad ,  ZHANG, Yiran ,  MARCOGLIESE, Francis ,  WHEELER, Tobias, Daniel ,  BHARADWAJ, Rajiv

IPC分类号： B01L3/00

公开(公告)号：EP4153984A1

公开(公告)日：2023-03-29

申请号：EP21736063.5

申请日：2021-05-18

申请人： 10X Genomics, Inc.

发明人： TENTORI, Augusto Manuel ,  KIM, Hanyoup ,  BAVA, Felice Alessio ,  BHARADWAJ, Rajiv

IPC分类号： G01N27/447

公开(公告)号：EP4146819A1

公开(公告)日：2023-03-15

申请号：EP21727672.4

申请日：2021-04-30

申请人： 10X Genomics, Inc.

发明人： TENTORI, Augusto Manuel ,  KIM, Hanyoup ,  BHARADWAJ, Rajiv

IPC分类号： C12Q1/6837

公开(公告)号：EP3844304A2

公开(公告)日：2021-07-07

申请号：EP19765928.7

申请日：2019-08-27

申请人： 10X Genomics, Inc.

发明人： RAMACHANDRAN IYER, Eswar Prasad ,  SCHNALL-LEVIN, Michael ,  TENTORI, Augusto Manuel ,  MIKKELSEN, Tarjei Sigurd ,  HINDSON, Benjamin ,  LUCERO, Michael Ybarra ,  BHARADWAJ, Rajiv ,  YIN, Yifeng ,  BENT, Zachary ,  CHEW, Jennifer ,  FREY, Meghan L. F. ,  KVASTAD, Linda ,  LUNDEBERG, Joakim ,  VILLACAMPA, Eva Gracia

IPC分类号： C12Q1/6837 ,  C12Q1/6841

公开(公告)号：EP3545089A1

公开(公告)日：2019-10-02

申请号：EP18744153.0

申请日：2018-01-30

申请人： 10X Genomics, Inc.

发明人： BELHOCINE, Kamila ,  BHARADWAJ, Rajiv ,  HINDSON, Christopher ,  SCHNALL-LEVIN, Michael ,  LIN, Bill ,  MAKAREWICZ, Anthony ,  PATEL, Pranav ,  PFEIFFER, Katherine ,  PRICE, Andrew D. ,  RAHIMI LENJI, Mohammad ,  WHEELER, Tobias Daniel ,  YIN, Yifeng

IPC分类号： C12N15/09 ,  C12Q1/68 ,  C12Q1/6806

公开(公告)号：EP4310183A2

公开(公告)日：2024-01-24

申请号：EP23174169.5

申请日：2018-01-30

申请人： 10X Genomics, Inc.

发明人： BELHOCINE, Kamila ,  BHARADWAJ, Rajiv ,  HINDSON, Christopher ,  SCHNALL-LEVIN, Michael ,  LIN, Bill ,  MAKAREWICZ, Anthony ,  PATEL, Pranav ,  PFEIFFER, Katherine ,  PRICE, Andrew D. ,  RAHIMI LENJI, Mohammad ,  WHEELER, Tobias Daniel ,  YIN, Yifeng

IPC分类号： C12N15/10

摘要： Provided herein are methods and systems for sample preparation techniques that allow amplification, e.g., whole genome amplification, reverse transcription, amplification of cellular nucleic acids, etc., and sequencing of single cells, which may be of interest. The methods and systems generally operate by bringing together a first liquid phase comprising a plurality of biological particles, a second liquid phase comprising gel beads, and a third immiscible phase. The liquid phases may interact to form partitions. The methods and systems may be configured to allow the implementation of a single operation or multi-operation chemical and/or biochemical processing within the partitions. Another aspect of the disclosure provides a composition comprising a cell bead of a plurality of cell beads and a barcode bead of a plurality of barcode beads. Also provided herein are kits for analyzing individual cells or viruses or small populations of cells or viruses.

公开(公告)号：EP4213993A2

公开(公告)日：2023-07-26

申请号：EP21791151.0

申请日：2021-09-17

申请人： 10X Genomics, Inc.

发明人： TENTORI, Augusto Manuel ,  KIM, Hanyoup ,  XING, Siyuan ,  BHARADWAJ, Rajiv ,  LIN, Bill Kengli ,  BAVA, Felice Alessio ,  ALIMSIJAH, Pratomo Putra ,  MARINDRA, Hendricus ,  RUSSELL, Spontaneous Rose McKnight ,  MIKHAIEL, Nabil

IPC分类号： B01L9/00

公开(公告)号：EP4164796A1

公开(公告)日：2023-04-19

申请号：EP21821522.6

申请日：2021-06-10

申请人： 10x Genomics, Inc.

发明人： KIM, Hanyoup ,  TENTORI, Augusto Manuel ,  XING, Siyuan ,  BHARADWAJ, Rajiv ,  LIN, Bill Kengli ,  BAVA, Felice Alessio ,  ALIMSIJAH, & x9;Pratomo Putra ,  MIKHAIEL, Nabil

IPC分类号： B01L3/00 ,  B01L7/00 ,  C12Q1/68 ,  G01N33/569