-
公开(公告)号：EP0656068A4
公开(公告)日：1996-05-29
申请号：EP93915557
申请日：1993-07-26
CPC分类号： C12Q1/6834 , C12Q1/6816 , C12Q1/6844 , C12Q1/686 , C12Q2565/501 , C12Q2531/107
摘要： PCT No. PCT/AU93/00379 Sec. 371 Date Jan. 24, 1995 Sec. 102(e) Date Jan. 24, 1995 PCT Filed Jul. 26, 1993 PCT Pub. No. WO94/02634 PCT Pub. Date Feb. 3, 1994This method for detecting a target nucleic acid sequence involves amplification and detection in the same vessel and comprises: (a) amplification of the target nucleic sequence in a vessel which is provided with a solid phase capture probe comprising a nucleic acid sequence capable of hybridizing to at least a portion of said amplified target nucleic acid sequence, said capture probe being incapable of participating or not participating in standard nucleic acid sequence amplification processes, (b) bringing a sample suspected of comprising said target nucleic acid sequence into contact with said capture probe under conditions which allow said amplified target nucleic acid sequence to be bound by said capture probe, and (c) detecting the presence of bound target nucleic acid sequence. In a further aspect, the present invention provides an assay system or kit, for detecting a target nucleic acid sequence in a sample suspected of comprising said target nucleic acid sequence, comprising: (a) a capture probe comprising a nucleic acid sequence capable of hydridizing to at least a portion of said amplified target nucleic acid sequence, said capture probe being immobilized on a solid phase support which forms a part of or is insertable into a container for the sample, and said capture probe being incapable of participating in standard nucleic acid sequence amplification processes, (b) reagents for amplification of said target nucleic acid sequence, and (c) means for detecting said target nucleic acid sequence, when bound by said capture probe.
-
公开(公告)号：EP0672173A4
公开(公告)日：1997-06-04
申请号：EP92922138
申请日：1992-10-30
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6834 , C12Q2565/537 , C12Q2531/113 , C12Q2531/143 , C12Q2531/137
摘要： An assay and method of use thereof for amplification and detection of target nucleic acids in patient samples, which is rapid, simple and accurate is described. The assay and method utilizes any one of a variety of amplification methods, in combination with primers immobilized onto either the container containing the patient sample, such as a microtiter well plate, or a dipstick which is readily immersible into the container. Amplification occurs in the presence of both the first, immobilized primer and a second, labelled primer which hybridizes to the target nucleic acid in the opposite direction to the first. Examples demonstrate the effectiveness of the method in detecting a specific species of mycoplasma, as well as the gene characteristic of cystic fibrosis.
-
搜索结果
2 条记录 (耗时 0.01 秒)
