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    METHOD FOR DETECTING TARGET BASE SEQUENCE USING COMPETITIVE PRIMER
    5.
    发明公开
    METHOD FOR DETECTING TARGET BASE SEQUENCE USING COMPETITIVE PRIMER 有权
    VERFAHREN ZUR ERKENNUNG VON ZIELBASISSEQUENZEN MITHILFE KONKURRIERENDER PRIMER

    公开(公告)号：EP2551356A4

    公开(公告)日：2013-09-11

    申请号：EP11759408

    申请日：2011-03-23

    申请人： TOPPAN PRINTING CO LTD

    发明人： MAKINO YOICHI ,  YAMANE AKIO ,  NAKAYAMA MASATO

    IPC分类号： C12Q1/68 ,  C12N15/09

    CPC分类号： C12Q1/6869 ,  C12Q1/6827 ,  C12Q1/6858 ,  C12Q2531/119 ,  C12Q2537/161 ,  C12Q2531/143 ,  C12Q2531/113 ,  C12Q2525/161 ,  C12Q2525/185 ,  C12Q2525/204 ,  C12Q2535/125 ,  C12Q2549/101 ,  C12Q2563/107 ,  C12Q2565/101

    摘要： Disclosed is a method of detecting a target base sequence having a polymorphic base, the method including: (a) a step of adding to a nucleic acid sample having a target nucleic acid that includes a base sequence including the target base sequence: at least one type of detection primer, at least one type of competitive primer, and at least one type of common primer; (b) a step of annealing the detection primer and the competitive primer to the target nucleic acid in a competitive manner, thereby synthesizing an extension product A; (c) a step of annealing the common primer to the extension product A obtained in the step (b) or in the following step (d), thereby synthesizing an extension product B; (d) a step of annealing the detection primer or the competitive primer to the extension product B obtained in the previous step (c), thereby synthesizing the extension product A; and (e) a step of detecting the extension product A or the extension product B.

    摘要翻译： 公开了一种检测具有多态性碱基的靶碱基序列的方法，该方法包括：（a）向具有靶核酸的核酸样品添加步骤，所述靶核酸包括含有靶碱基序列的碱基序列：至少一个 类型的检测引物，至少一种类型的竞争性引物和至少一种类型的通用引物; （b）以竞争的方式将检测用引物和竞争性引物与靶核酸退火而合成延伸产物A的工序; （c）将通用引物退火至在步骤（b）或在随后的步骤（d）中获得的延伸产物A的步骤，由此合成延伸产物B; （d）将检测用引物或竞争性引物退火至前一步骤（c）中获得的延伸产物B，从而合成延伸产物A的步骤; 和（e）检测延伸产品A或延伸产品B的步骤。

    METHOD FOR MEASURING CYTOKERATIN-19 MRNA
    6.
    发明公开
    METHOD FOR MEASURING CYTOKERATIN-19 MRNA 有权
    测量细胞角蛋白-19MRNA的方法

    公开(公告)号：EP2351851A1

    公开(公告)日：2011-08-03

    申请号：EP09829212.1

    申请日：2009-11-30

    申请人： Tosoh Corporation

    发明人： OMOTO, Daisuke ,  SAITO, Juichi ,  OONAKA, Satoru ,  HAYASHI, Toshinori

    IPC分类号： C12Q1/68 ,  C12N15/09

    CPC分类号： C12Q1/6853 ,  C12Q1/6865 ,  C12Q2525/143 ,  C12Q2545/114 ,  C12Q2561/113 ,  C12Q2531/143 ,  C12Q2521/107

    摘要： Disclosed is a method of amplifying and detecting cytokeratin 19 mRNA in RNA amplification process, comprising: a step for forming a double-stranded DNA containing a promoter sequence with a reverse transcriptase by use of a combination of oligonucleotides consisting of a first primer having a sequence homologous to a portion of cytokeratin 19 mRNA and a second primer having a complementary sequence, wherein the promoter sequence is added to the 5'-end of either the first primer or the second primer, forming an RNA transcription product by use of an RNA polymerase with using the double-stranded DNA as template, and forming the double-stranded DNA by use of a reverse transcriptase by continuing to use the RNA transcription product as a template of DNA synthesis, by measuring an amount of amplified RNA produced over time with an oligonucleotide probe designed so that signal properties change with the formation of a complementary double strand with the amplified RNA.

    摘要翻译： 本发明公开了在RNA扩增过程中扩增和检测细胞角蛋白19mRNA的方法，其包括：用逆转录酶形成含有启动子序列的双链DNA的步骤，其中使用由具有序列的第一引物 与细胞角蛋白19 mRNA的一部分同源，以及具有互补序列的第二引物，其中启动子序列被添加到第一引物或第二引物的5'末端，通过使用RNA聚合酶形成RNA转录产物 以双链DNA为模板，通过继续使用RNA转录产物作为DNA合成的模板，通过使用逆转录酶形成双链DNA，通过测量随时间产生的扩增RNA的量 寡核苷酸探针，其设计使得信号特性随着与扩增的RNA形成互补双链而改变。

    IMPROVED METHOD OF DETECTING NOROVIRUS RNA
    7.
    发明公开
    IMPROVED METHOD OF DETECTING NOROVIRUS RNA 审中-公开
    VERBESSERTES VERFAHRENFÜRDEN NACHWEIS VON NOROVIRUS-RNA

    公开(公告)号：EP2172561A1

    公开(公告)日：2010-04-07

    申请号：EP08777360.2

    申请日：2008-06-12

    申请人： Tosoh Corporation

    发明人： MASUDA, Noriyoshi ,  UNE, Kurando ,  SAITO, Juichi ,  HAYASHI, Toshinori

    IPC分类号： C12Q1/68 ,  C12N15/09 ,  G01N21/78 ,  G01N33/569

    CPC分类号： C12Q1/701 ,  C12Q2563/173 ,  C12Q2531/143 ,  C12Q2521/107

    摘要： The amount of an RNA transcription product amplified in an RNA amplification process is measured using a nucleic acid probe labeled with an intercalating fluorescent dye. The RNA amplification process comprises the steps of using at least two sets of primer pairs comprising a first primer and a second primer (in which one of these primers carries a promoter sequence added to the 5' end thereof), both of which have high hybridization efficiency to a nucleic acid sequence that is homologous to or complementary to each norovirus genotype RNA; forming a double-stranded DNA containing the promoter sequence with a reverse transcriptase; forming an RNA transcription product with an RNA polymerase by using the double-stranded DNA as a template; and forming the double-stranded DNA by successively using the RNA transcription product as a template in the DNA synthesis with the reverse transcriptase.

    摘要翻译： 使用用插层荧光染料标记的核酸探针测量在RNA扩增过程中扩增的RNA转录产物的量。 RNA扩增方法包括以下步骤：使用至少两组包含第一引物和第二引物的引物对（其中这些引物中的一个携带加入其5'末端的启动子序列），两者都具有高杂交 与每种诺如病毒基因型RNA同源或互补的核酸序列的效率; 用逆转录酶形成含有启动子序列的双链DNA; 通过使用双链DNA作为模板与RNA聚合酶形成RNA转录产物; 并通过在逆转录酶的DNA合成中连续使用RNA转录产物作为模板形成双链DNA。

    METHODS AND COMPOSITIONS FOR NUCLEIC ACID AMPLIFICATION
    8.
    发明公开
    METHODS AND COMPOSITIONS FOR NUCLEIC ACID AMPLIFICATION 有权
    方法和组合物核酸AMPLIZIERUNG

    公开(公告)号：EP2121956A2

    公开(公告)日：2009-11-25

    申请号：EP07869693.7

    申请日：2007-12-20

    申请人： GEN-PROBE INCORPORATED

    发明人： BRENTANO, Steven T. ,  CARLSON, James D. ,  LYAKHOV, Dmitry ,  NELSON, Norman C. ,  ARNOLD, Lyle, J., Jr

    IPC分类号： C12P19/34

    CPC分类号： C12Q1/6881 ,  C12Q1/6834 ,  C12Q1/6844 ,  C12Q1/6865 ,  C12Q2525/155 ,  C12Q2525/186 ,  C12Q2525/197 ,  C12Q2531/143 ,  C12Q2565/543

    摘要： There is described a target capture reaction mixture for separating a target nucleic acid from a sample, the reaction mixture comprising: a. a target specific universal (TSU) primer complex made up of i. a TSU promoter oligonucleotide comprising a 5' promoter sequence, an internal first universal sequence (U1), and a 3' first target specific sequence (TS1) that binds specifically to a target sequence contained in a target nucleic acid, wherein the TSU promoter oligonucleotide is a TSU promoter primer that has a 3' terminus that is capable of being extended by a polymerase, or is a TSU promoter provider oligonucleotide that has a blocked 3' terminus that is incapable of being extended by a polymerase, directly or indirectly joined to, ii. a TSU non-promoter primer oligonucleotide made up of a 5' second universal sequence (U2) and a 3' second target specific sequence (TS2) which is different from the TS1, wherein the TSU promoter oligonucleotide is joined to the TSU non-promoter primer via: (A) a covalent linkage that is a polynucleotide linker sequence or a non-nucleotide abasic linker compound; (B) a hybridization complex between a first sequence on the TSU promoter oligonucleotide and a second sequence on the TSU non-promoter primer that is complementary to the first sequence on the TSU promoter oligonucleotide; or (C) a hybridization complex that includes an S-oligonucleotide that contains a first sequence complementary to a sequence in the TSU promoter oligonucleotide and a second sequence complementary to a sequence in the TSU non-promoter primer oligonucleotide; and b. a target specific capture oligonucleotide that contains a target specific sequence (TS3) that hybridizes specifically to a sequence in the target nucleic acid that is different from the sequence in the target nucleic acid that hybridizes to the TS sequence of the TSU promoter oligonucleotide or the TS sequence of the TSU non-promoter primer, and contains a means for binding the target nucleic acid to a solid support.

    MUTATION DETECTION USING PEPTIDE TAGGED IN VITRO SYNTHESIZED PROTEINS
    9.
    发明授权
    MUTATION DETECTION USING PEPTIDE TAGGED IN VITRO SYNTHESIZED PROTEINS 失效
    突变借助体外检测中合成的蛋白质用肽MARK

    公开(公告)号：EP0931160B1

    公开(公告)日：2008-12-17

    申请号：EP96931558.9

    申请日：1996-09-13

    申请人： Garvin, Alex M.

    发明人： Garvin, Alex M.

    IPC分类号： C12P19/34 ,  G01N33/543 ,  C12Q1/68

    CPC分类号： C12Q1/68 ,  C12Q1/6804 ,  C12Q2531/143 ,  C12Q2525/149

    摘要： A process for detecting protein altering mutations in genes. Coding sequence of the gene is PCR amplified with a 5' primer that contains at its 5' end a polymerase binding site, a translation initiation site, and an in frame sequence coding for a peptide tag, followed by the in frame 5' end of the test sequence. After PCR amplification of the test sequence, the PCR product is used as template to make mRNA in an in vitro transcription reaction using an RNA polymerase that recognizes the polymerase binding site incorporated into the 5' PCR primer. The mRNA is then used as template to make protein in an in vitro translation reaction. The protein encoded by the test sequence has at its amino terminus a peptide tag that can be used to either detect the protein, or to purify the protein for further analysis.