公开(公告)号：EP1383891A2

公开(公告)日：2004-01-28

申请号：EP02733179.2

申请日：2002-04-19

申请人： ADEREGEM (Association pour le Développement de la Recherche en Génétique Moléculaire

发明人： CHAMBON, Pierre ,  GHYSELINCK, Norbert, B. ,  SCHNÜTGEN, Frank

IPC分类号： C12N15/11 ,  C12N15/09 ,  C12N15/63 ,  C12N15/90 ,  C12N5/10 ,  C12N1/21 ,  C12N1/19 ,  A01H5/00 ,  A01K67/027

CPC分类号： C12N15/90 ,  C12N15/63 ,  C12N2800/30 ,  C12N2800/60 ,  C12N2840/20 ,  C12N2840/203 ,  C12N2840/44

摘要： The invention relates to a method for the stable inversion of a DNA fragment upon recombinase-mediated rearrangements using two sets of two incompatible site-specific recombinase targeting sites (SSRTS) in the same order but in reverse orientation flanking said DNA fragment to be inverted. The invention also relates to a method for the stable inversion of said DNA fragment upon rearrangement mediated by a recombinase such as Cre recombinase. The invention also relates to a method for obtaining a transgenic cell of which at least one allele of a DNA sequence of interest is invalidated by a process of conditional deletion and the genome of which comprises a reporter gene inserted at the place of the DNA fragment deleted by said process of conditional deletion. The invention also concerns a method to generate targeting sites to perform site-specific recombination mediated cassette exchange. The corresponding vectors, host cells, and transgenic animals are claimed.