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公开(公告)号：EP0301749B1
公开(公告)日：1994-03-02
申请号：EP88306613.6
申请日：1988-07-20
申请人： AGRACETUS, INC.
CPC分类号： C12N15/87 , C12N15/8207
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2.发明授权Plant-cell transformation by accelerated particles coated with DNA and apparatus therefor. 失效通过加速粒子，涂有DNA及其装置的装置的植物细胞的转化。
公开(公告)号：EP0270356B1
公开(公告)日：1994-06-01
申请号：EP87310612.4
申请日：1987-12-02
申请人： AGRACETUS, INC.
CPC分类号： C12N15/87 , A01H1/02 , C12N15/8207
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3.发明授权Effecting somatic changes in plants through the use of negative strand RNAS 无效通过使用negativstrangigen的RNA引起植物生理变化。
公开(公告)号：EP0223399B1
公开(公告)日：1994-04-20
申请号：EP86307850.7
申请日：1986-10-10
申请人： AGRACETUS, INC.
CPC分类号： C12N15/1131 , C12N15/1137 , C12N15/8218 , C12N2310/111 , C12Y105/01019
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4.发明公开Plant-cell transformation by accelerated particles coated with DNA and apparatus therefor. 失效通过加速粒子，涂有DNA及其装置的装置的植物细胞的转化。
公开(公告)号：EP0270356A2
公开(公告)日：1988-06-08
申请号：EP87310612.4
申请日：1987-12-02
申请人： AGRACETUS, INC.
CPC分类号： C12N15/87 , A01H1/02 , C12N15/8207
摘要： A method and apparatus is disclosed for the genetic transformation of plants and plant lines by pollen mediated transformation. Foreign genes are introduced into pollen by coating on carrier particles which are physically accelerated into plant pollen. The treated plant pollen is then hand pollinated and the progeny are recovered, a portion of which will contain in their genome the foreign gene. The procedure may be used to create genetically engineered maize plant and lines.
摘要翻译： 的方法和设备是游离缺失盘用于通过花粉介导的转化的植物和植物系的基因转化。 外源基因是通过在其上物理加速进入植物花粉载体颗粒的涂料将引入花粉。 经处理的植物的花粉然后手工授粉和后代被回收，一部分whichwill的包含在它们的基因组中的外源基因。 该过程可被用于创建遗传工程改造玉米植物和线条。
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公开(公告)号：EP0301749A3
公开(公告)日：1990-11-28
申请号：EP88306613.6
申请日：1988-07-20
申请人： AGRACETUS, INC.
CPC分类号： C12N15/87 , C12N15/8207
摘要： A method and apparatus is disclosed for the genetic transformation of soybean plants and plant lines by particle mediated transformation. Foreign genes are introduced into regenerable soybean tissues by coating on carrier particles which are physically accelerated into plant tissues. The treated plant tissues are then recovered and regenerated into whole sexually mature plants. The progeny are recovered from seed set by these plants and a portion of these progeny will contain in their genome the foreign gene. The procedure may be used to create novel genetically engineered soybean plants and lines.
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6.发明公开Effecting somatic changes in plants through the use of negative strand RNAS 无效Hervorrufung physischerVeränderungenin Pflanzen durch Verwendung von negativstrangigen RNAs。
公开(公告)号：EP0223399A1
公开(公告)日：1987-05-27
申请号：EP86307850.7
申请日：1986-10-10
申请人： AGRACETUS, INC.
CPC分类号： C12N15/1131 , C12N15/1137 , C12N15/8218 , C12N2310/111 , C12Y105/01019
摘要： A strategy is disclosed for effecting useful somatic changes in plants by causing the transcription in the plant cells of negative RNA strands which are substantially complementary to a target RNA strand. The target RNA strand can be an mRNA transcript created in gene expression, a viral RNA, or other RNA present in the plant cells. The negative RNA strand is complementary to at least a portion of the target RNA strand to inhibit its activity in vivo .
摘要翻译： 公开了通过引起与靶RNA链基本上互补的负RNA链的植物细胞中的转录来实现植物有用体细胞变化的策略。 靶RNA链可以是在基因表达中产生的mRNA转录物，病毒RNA或存在于植物细胞中的其它RNA。 负RNA链与目标RNA链的至少一部分互补以抑制其在体内的活性。
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7.发明公开
公开(公告)号：EP0430511A1
公开(公告)日：1991-06-05
申请号：EP90312498.0
申请日：1990-11-16
申请人： AGRACETUS, INC.
CPC分类号： C12N15/8277 , A01H5/10 , C12N9/1029 , C12N15/8207
摘要： A gene for resistance to a class of herbicides, the glutamine synthetase inhibitors, was isolated from a microbial source and transformed into soybean plants. The gene for resistance to these herbicides used, the Bar gene is not only useful to make soybean plants which are resistant to the herbicides for field use, but also is useful as a selectable marker in plant transformation techniques. The gene was transformed into soybean through the use of a particle acceleration transformation method.
摘要翻译： 从一种微生物来源中分离出一类抗除草剂谷氨酰胺合成酶抑制剂的基因，并转化成大豆植物。 使用这些除草剂的抗性基因，Bar基因不仅可用于制备对除草剂具有抗性的大豆植物用于野外使用，而且可用作植物转化技术中的选择性标记。 通过使用粒子加速转化方法将基因转化成大豆。
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8.发明公开Plant-cell transformation by accelerated particles coated with DNA and apparatus therefor. 失效通过用DNA包被的加速颗粒进行植物细胞转化及其装置。
公开(公告)号：EP0270356A3
公开(公告)日：1990-03-07
申请号：EP87310612.4
申请日：1987-12-02
申请人： AGRACETUS, INC.
CPC分类号： C12N15/87 , A01H1/02 , C12N15/8207
摘要： A method and apparatus is disclosed for the genetic transformation of plants and plant lines by pollen mediated transformation. Foreign genes are introduced into pollen by coating on carrier particles which are physically accelerated into plant pollen. The treated plant pollen is then hand pollinated and the progeny are recovered, a portion of which will contain in their genome the foreign gene. The procedure may be used to create genetically engineered maize plant and lines.
摘要翻译： 公开了一种通过花粉介导的转化对植物和植物系进行遗传转化的方法和设备。 将外来基因通过涂布在物理加速成植物花粉的载体颗粒上而引入花粉中。 然后将处理过的植物花粉人工授粉并回收后代，其中一部分将在其基因组中包含外源基因。 该程序可用于创建基因工程玉米植株和系。
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9.发明公开Particle-mediated transformation of soybean plants and lines 无效转型von Sojabohnenpflanzen und Zellinien mit Hilfe von Partikeln。
公开(公告)号：EP0301749A2
公开(公告)日：1989-02-01
申请号：EP88306613.6
申请日：1988-07-20
申请人： AGRACETUS, INC.
CPC分类号： C12N15/87 , C12N15/8207
摘要： A method and apparatus is disclosed for the genetic transformation of soybean plants and plant lines by particle mediated transformation. Foreign genes are introduced into regenerable soybean tissues by coating on carrier particles which are physically accelerated into plant tissues. The treated plant tissues are then recovered and regenerated into whole sexually mature plants. The progeny are recovered from seed set by these plants and a portion of these progeny will contain in their genome the foreign gene. The procedure may be used to create novel genetically engineered soybean plants and lines.
摘要翻译： 公开了通过粒子介导的转化进行大豆植物和植物品系的遗传转化的方法和装置。 外来基因通过涂覆在物理加速到植物组织中的载体颗粒上引入可再生大豆组织中。 然后将经处理的植物组织回收并再生成整个性成熟植物。 从这些植物的种子中回收后代，这些后代的一部分将在其基因组中含有外来基因。 该方法可用于产生新颖的基因工程大豆植物和品系。
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