利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

3052 条记录 (耗时 0.021 秒)

    DNA POLYMERASE MUTANT AND USE THEREOF
    1.
    发明公开
    DNA POLYMERASE MUTANT AND USE THEREOF 审中-公开

    公开(公告)号：EP4455279A1

    公开(公告)日：2024-10-30

    申请号：EP21968652.4

    申请日：2021-12-24

    申请人： BGI Shenzhen

    发明人： ZHANG, Xiaohong ,  LAN, Xi ,  XIE, Qingqing ,  XI, Feng ,  ZHENG, Yue ,  DONG, Yuliang ,  ZHANG, Wenwei

    IPC分类号： C12N9/12 ,  C12N15/54 ,  C12Q1/686

    摘要： The present invention relates to the field of biotechnology, in particular to a DNA polymerase mutant and the use thereof. Compared with a wild type, the mutant provided in the present invention has a significantly improved DNA polymerization activity, significantly improved affinity to DNA and amplification uniformity; and has good effects in the amplification of multiplex PCR, high-GC templates and complex templates. A hot-start-version DNA polymerase is further prepared by means of using antibodies or chemical modifications, wherein the yield of the DNA polymerase is significantly improved, while the primer dimer is significantly decreased. The prepared mutant hot-start DNA polymerase can be applied to PCR amplification of multiplex PCR high-GC templates and complex templates.

    SUCROSE SYNTHETASE AND USE THEREOF
    6.
    发明公开
    SUCROSE SYNTHETASE AND USE THEREOF 审中-实审

    公开(公告)号：EP4379050A1

    公开(公告)日：2024-06-05

    申请号：EP22848384.8

    申请日：2022-07-21

    申请人： Abiochem Biotechnology (Group) Co., Ltd.

    发明人： WU, Yan ,  TIAN, Zhenhua ,  WANG, Shu ,  ZHENG, Xiaofu ,  XING, Litong

    IPC分类号： C12N9/10 ,  C12N15/54 ,  C12N15/63 ,  C12P19/00

    CPC分类号： C12P19/56 ,  C12N9/10 ,  C12R2001/1920210501 ,  C12N15/70 ,  C12N15/74 ,  C12N15/63 ,  C12P19/00

    摘要： Provided are a sucrose synthetase and the use thereof. Compared with SEQ ID NO: 11, an amino acid sequence of the sucrose synthetase contains differences in amino acid residues selected from one or more of the following residue positions: an amino acid at position 36 being V, an amino acid at position 43 being E, an amino acid at position 179 being A, an amino acid at position 395 being R, an amino acid at position 447 being P, an amino acid at position 455 being L, and an amino acid at position 654 being R; and the sucrose synthetase has an activity not lower than that of a sucrose synthetase having an amino acid sequence as shown in SEQ ID NO: 11. By means of catalyzing the synthesis of rebaudioside A, rebaudioside D or rebaudioside M by using a glucosyltransferase and the sucrose synthetase in a combined manner, a cascade reaction is achieved, thereby providing a variety of possibilities for the selection of raw materials.