公开(公告)号：EP4345166A3

公开(公告)日：2024-07-17

申请号：EP23200019.0

申请日：2023-09-27

申请人： AJINOMOTO CO., INC.

发明人： HAMANO, Chie ,  ITO, Yoshihiro ,  EBARA, Naoki ,  INOUE, Kota ,  ONO, Yukiko ,  ISHIDA, Rihito ,  ONISHI, Fumito ,  HARA, Yoshihiko

IPC分类号： C12P13/10 ,  C12N9/88 ,  C12P13/14 ,  C12P13/24

CPC分类号： C12P13/14 ,  C12P13/10 ,  C12P13/24 ,  C12Y401/01005 ,  C12N9/88 ,  C12R2001/1920210501 ,  C12Y101/01004 ,  C12N9/0006 ,  C12N15/74 ,  C12R2001/0120210501

摘要： A method for producing an L-amino acid such as L-glutamic acid is provided. An L-amino acid is produced by culturing in a culture medium a bacterium belonging to the family Enterobacteriaceae and having an L-amino acid-producing ability, and collecting the L-amino acid from the culture medium and/or cells of the bacterium, wherein the bacterium has been modified to have one or more modifications selected from the modifications (A) to (F) shown below:
(A) modification of reducing the activity of a BudA protein;
(B) modification of reducing the activity of a BudB protein;
(C) modification of reducing the activity of a BudC protein;
(D) modification of reducing the activity of a PAJ_3461 protein;
(E) modification of reducing the activity of a PAJ 3462 protein; and
(F) modification of reducing the activity of a PAJ_3463 protein.

公开(公告)号：EP4345166A2

公开(公告)日：2024-04-03

申请号：EP23200019.0

申请日：2023-09-27

申请人： AJINOMOTO CO., INC.

发明人： HAMANO, Chie ,  ITO, Yoshihiro ,  EBARA, Naoki ,  INOUE, Kota ,  ONO, Yukiko ,  ISHIDA, Rihito ,  ONISHI, Fumito ,  HARA, Yoshihiko

IPC分类号： C12P13/10 ,  C12N9/88 ,  C12P13/14 ,  C12P13/24

摘要： A method for producing an L-amino acid such as L-glutamic acid is provided. An L-amino acid is produced by culturing in a culture medium a bacterium belonging to the family Enterobacteriaceae and having an L-amino acid-producing ability, and collecting the L-amino acid from the culture medium and/or cells of the bacterium, wherein the bacterium has been modified to have one or more modifications selected from the modifications (A) to (F) shown below:
(A) modification of reducing the activity of a BudA protein;
(B) modification of reducing the activity of a BudB protein;
(C) modification of reducing the activity of a BudC protein;
(D) modification of reducing the activity of a PAJ_3461 protein;
(E) modification of reducing the activity of a PAJ 3462 protein; and
(F) modification of reducing the activity of a PAJ_3463 protein.

公开(公告)号：EP3438264A1

公开(公告)日：2019-02-06

申请号：EP17775532.9

申请日：2017-03-31

申请人： Ajinomoto Co., Inc.

发明人： KITAZAWA, Manabu ,  SUGIMOTO, Nao ,  KODAMA, Riho ,  NAGAHIKO, Takeshi ,  ITO, Yoshihiro ,  NAKASE, Kentaro ,  TAKASHIMA, Yoshinori ,  MINE, Ayako ,  KUWABARA, Yoko ,  TAGAMI, Uno ,  SUGIKI, Masayuki

IPC分类号： C12N15/09 ,  C07K14/435 ,  C07K19/00 ,  C12M3/04 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/0735 ,  C12N5/10

摘要： A technique for efficiently culturing cells such as stem cells is provided. Cells such as stem cells are cultured using a cell culture vessel coated with a fibroin-like protein inserted with a cell adhesion sequence containing RGD (Arg-Gly-Asp).