公开(公告)号：EP3147368A1

公开(公告)日：2017-03-29

申请号：EP15796551.8

申请日：2015-05-21

申请人： Ajinomoto Co., Inc. ,  Spiber Inc.

发明人： NAKASE, Kentaro

IPC分类号： C12P21/02 ,  C12N15/09

CPC分类号： C07K14/43518 ,  C12N15/09 ,  C12N15/70 ,  C12P21/02

摘要： A method for producing a fibroin-like protein is provided. A fibroin-like protein is produced by a method for producing a fibroin-like protein comprising culturing Escherichia coli having a gene encoding the fibroin-like protein in a medium, inducing expression of the gene encoding the fibroin-like protein, and collecting the fibroin-like protein, wherein accumulation of an organic acid at the time of inducing the expression is reduced.

摘要翻译： 提供丝心蛋白样蛋白质的制造方法。 通过生产丝心蛋白样蛋白质的方法制备丝心蛋白样蛋白质，包括在培养基中培养具有编码丝心蛋白样蛋白质的基因的大肠杆菌，诱导编码丝心蛋白样蛋白质的基因的表达，并收集丝心蛋白 样蛋白质，其中诱导表达时有机酸的积累减少。

公开(公告)号：EP3382031A1

公开(公告)日：2018-10-03

申请号：EP16868608.7

申请日：2016-11-24

申请人： Ajinomoto Co., Inc. ,  Spiber Inc.

发明人： NAKASE, Kentaro ,  TAKASHIMA, Yoshinori ,  NAGAHIKO, Takeshi

IPC分类号： C12P21/02 ,  C12N15/09

CPC分类号： C07K14/43518 ,  C07K2319/02 ,  C07K2319/034 ,  C07K2319/035 ,  C12N15/09 ,  C12N15/71 ,  C12P21/02 ,  D01F4/02

摘要： A method for producing a fibroin-like protein is provided. A fibroin-like protein is produced by a method for producing a fibroin-like protein comprising culturing Escherichia coli having a gene encoding the fibroin-like protein in a medium, inducing expression of the gene encoding the fibroin-like protein, and collecting the fibroin-like protein, wherein accumulation of an organic acid at the time of inducing the expression is reduced, and wherein the gene is expressed under control of a tryptophan promoter.

公开(公告)号：EP3438264A1

公开(公告)日：2019-02-06

申请号：EP17775532.9

申请日：2017-03-31

申请人： Ajinomoto Co., Inc.

发明人： KITAZAWA, Manabu ,  SUGIMOTO, Nao ,  KODAMA, Riho ,  NAGAHIKO, Takeshi ,  ITO, Yoshihiro ,  NAKASE, Kentaro ,  TAKASHIMA, Yoshinori ,  MINE, Ayako ,  KUWABARA, Yoko ,  TAGAMI, Uno ,  SUGIKI, Masayuki

IPC分类号： C12N15/09 ,  C07K14/435 ,  C07K19/00 ,  C12M3/04 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/0735 ,  C12N5/10

摘要： A technique for efficiently culturing cells such as stem cells is provided. Cells such as stem cells are cultured using a cell culture vessel coated with a fibroin-like protein inserted with a cell adhesion sequence containing RGD (Arg-Gly-Asp).

公开(公告)号：EP3147369A1

公开(公告)日：2017-03-29

申请号：EP15796845.4

申请日：2015-05-21

申请人： Ajinomoto Co., Inc. ,  Spiber Inc.

发明人： NAKASE, Kentaro

IPC分类号： C12P21/02 ,  C12N1/00 ,  C12N1/21 ,  C12N15/09

CPC分类号： C07K14/43518 ,  C12N1/00 ,  C12N15/09 ,  C12N15/70 ,  C12P21/02

摘要： A method for producing a fibroin-like protein is provided. A fibroin-like protein is produced by a method for producing a fibroin-like protein comprising culturing Escherichia coli having a gene encoding the fibroin-like protein in a medium, inducing expression of the gene encoding the fibroin-like protein, and collecting the fibroin-like protein, wherein cell proliferation after inducing the expression is reduced.

摘要翻译： 提供丝心蛋白样蛋白质的制造方法。 通过生产丝心蛋白样蛋白质的方法制备丝心蛋白样蛋白质，包括在培养基中培养具有编码丝心蛋白样蛋白质的基因的大肠杆菌，诱导编码丝心蛋白样蛋白质的基因的表达，并收集丝心蛋白 样蛋白，其中诱导表达后的细胞增殖减少。