公开(公告)号：EP0733106A4

公开(公告)日：1998-06-10

申请号：EP95902007

申请日：1994-12-01

申请人： ALBRIGHT & WILSON AUSTRALIA

发明人： CHEN CHAO-GUANG ,  MAU SHAIO-LIM ,  DU HE ,  GANE ALISON M ,  BACIC ANTONY ,  CLARKE ADRIENNE E

IPC分类号： C12N15/09 ,  A23J1/00 ,  A61K38/00 ,  A61K47/48 ,  C07H21/04 ,  C07K14/415 ,  C07K16/16 ,  C12N5/10 ,  C12N15/82 ,  C12P21/02 ,  C12Q1/68 ,  C12R1/91 ,  C12N15/10 ,  A23L1/035 ,  A23L1/052 ,  A23L1/0562 ,  C12N15/29 ,  C12N15/74

CPC分类号： C07K14/415 ,  A23J1/007 ,  A61K38/00 ,  C12N15/8241

摘要： This invention provides plant arabinogalactan proteins (AGPs) and their genes. AGPs were isolated from Nicotiana alata, Nicotiana plumbaginafolia and Pyrus communis. Amino acid sequences of isolated AGP peptide molecules are presented. Isolated AGP molecules were used to synthesize oligonucleotide probes to prepare oligonucleotide primers for PCR or prepare RNA probes to screen cDNA libraries of N. alata, N. plumbaginafolia, and P. communis. cDNA clones encoding amino acid sequences of isolated AGP molecules were isolated. The invention presents for the first time an intact AGP amino acid sequence derived from a corresponding AGP gene. The instant invention further provides methods useful in obtaining AGP genes encoding an AGP peptide comprising a specific isolated hydroxyproline-rich (OAST-rich) sequence or a specific isolated hydroxyproline-poor sequence.