公开(公告)号：EP3066471A4

公开(公告)日：2017-05-10

申请号：EP14859021

申请日：2014-11-02

申请人： ALLIED INNOVATIVE SYSTEMS LLC

发明人： BYSTRYAK SIMON ,  SANTOCKYTE RASA

IPC分类号： G01N33/533 ,  G01N21/75 ,  G01N33/535 ,  G01N33/544

CPC分类号： G01N33/533 ,  C12Q1/28 ,  C12Q1/682 ,  C12Q1/703 ,  C12Q2600/158 ,  G01N33/5306 ,  G01N33/542 ,  G01N33/569 ,  G01N33/56911 ,  G01N33/56983 ,  G01N33/56988 ,  G01N33/56994

摘要： An improved assay for detecting an analyte in a fluid sample includes a step of conducting a photochemical reaction, in which a substrate conversion catalyzed by a photosensitizer into a product of the photochemical reaction is temporary inhibited when the reaction mixture is irradiated with a light at a wavelength within a light absorption spectrum of the photosensitizer. The photosensitizer (or an enzyme to catalyze producing thereof) is attached to an entity having an affinity to the analyte, such entity is bound to the analyte prior to irradiation. To achieve temporary inhibition, certain additives are used such as ascorbic acid or its derivatives. The assay may increase the sensitivity of ELISA 20- to 100-fold.

摘要翻译： 用于检测流体样品中的分析物的改进的测定法包括进行光化学反应的步骤，其中当用光照射反应混合物时，由光敏剂催化的底物转化成光化学反应的产物被暂时抑制 波长在光敏剂的光吸收光谱内。 光敏剂（或催化其产生的酶）附着到对分析物具有亲和力的实体上，这种实体在照射之前与分析物结合。 为了达到暂时抑制，使用某些添加剂如抗坏血酸或其衍生物。 该测定可以将ELISA的灵敏度提高20到100倍。

公开(公告)号：EP2972345A4

公开(公告)日：2016-10-05

申请号：EP14769059

申请日：2014-02-18

申请人： ALLIED INNOVATIVE SYSTEMS LLC

发明人： BYSTRYAK SIMON ,  SANTOCKYTE RASA

IPC分类号： G01N33/53 ,  G06M11/02

CPC分类号： G01N33/56972 ,  B01L3/5023 ,  B01L2200/04 ,  B01L2300/0825 ,  G01N33/5047 ,  G01N33/54313 ,  G01N2333/70514 ,  G01N2333/70517

摘要： A method for quantitatively measuring white blood cell (leukocyte) count and/or white blood cell (leukocyte) subsets count involves adding specific antibodies labeled with a marker to the biological fluid sample, capture of white blood cells from a fluid sample by a retainer, removal of other than leukocyte cells and other interfering substances by washing or using specific magnetic beads, and reading the result. The device for use in the present method includes a retainer for leukocyte cells and an absorption pad for taking up all excess washing solution flowing past the sample.