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    REAGENTS AND METHODS FOR ANALYSIS OF HIV
    5.
    发明公开
    REAGENTS AND METHODS FOR ANALYSIS OF HIV 审中-公开

    公开(公告)号：EP3350349A2

    公开(公告)日：2018-07-25

    申请号：EP16847412.0

    申请日：2016-09-16

    申请人： Siemens Healthcare Diagnostics Inc.

    发明人： PANDIT, Sunil ,  UZGIRIS, Arejas ,  PALMER, Lance

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07K14/16

    CPC分类号： C12Q1/703 ,  C07H21/02 ,  C12Q2600/106 ,  C12Q2600/158

    摘要： The present invention is based in part on the present inventors' appreciation that certain sequences within an HIV genome are more likely to successfully detect HIV across a breadth of HIV variants. The ability to detect and/or quantify the presence and/or load of HIV in a subject is important to, among other things, the diagnosis and treatment of infected individuals. The present invention is based, in part, on the discovery of oligonucleotide reagents that detectably amplify sequences from a greater breadth of HIV samples than certain prior reagents and/or that generate amplicons from HIV genomes from which certain prior reagents would not have generated amplicons. Oligonucleotide reagents as described herein provide unexpected benefits in the detection and/or quantification of the presence and/or load of HIV in a subject, and thereby in the diagnosis and treatment of HIV.

    DETECTION OF HIV-1 NUCLEIC ACIDS BY REVERSE-TRANSCRIPTION LOOP-MEDIATED ISOTHERMAL AMPLICATION
    8.
    发明公开
    DETECTION OF HIV-1 NUCLEIC ACIDS BY REVERSE-TRANSCRIPTION LOOP-MEDIATED ISOTHERMAL AMPLICATION 审中-公开
    逆转录循环介导等温扩增检测HIV-1核酸

    公开(公告)号：EP3215639A1

    公开(公告)日：2017-09-13

    申请号：EP15790793.2

    申请日：2015-10-28

    申请人： The United States of America, as represented by The Secretary, Department of Health and Human Services

    发明人： CURTIS, Kelly, A. ,  OWEN, Sherry, M. ,  NIEDZWIEDZ, Philip ,  RUDOLPH, Donna, L.

    IPC分类号： C12Q1/70

    CPC分类号： C12Q1/703 ,  C12Q2600/16

    摘要： Disclosed herein are methods of detecting HIV-1 nucleic acids in a sample (such as from a sample containing or suspected to contain HIV-1 nucleic acid). In some examples, the methods include loop-mediated isothermal amplification (LAMP) or reverse transcription-LAMP (RT- LAMP). In some examples, the methods include contacting a sample with one or more sets of LAMP primers specific for HIV-1 (such as LAMP primers specific for an HIV-1 integrase nucleic acid or LAMP primers specific for an HIV- 1 reverse transcriptase nucleic acid) under conditions sufficient to produce an amplification product and detecting the amplification product. Sets of LAMP primers for detection of HIV-1 integrase nucleic acids (such as SEQ ID NOs: 8-14 or 8-27) and HIV-1 reverse transcriptase nucleic acids (such as SEQ ID NOs: 1-7) are provided herein.

    摘要翻译： 本文公开了检测样品（例如来自含有或怀疑含有HIV-1核酸的样品）中的HIV-1核酸的方法。 在一些实例中，所述方法包括环介导的等温扩增（LAMP）或逆转录-LAMP（RT-LAMP）。 在一些实例中，所述方法包括使样品与一组或多组特异于HIV-1的LAMP引物（例如对HIV-1整合酶核酸特异的LAMP引物或对HIV-1逆转录酶核酸特异的LAMP引物 ）在足以产生扩增产物并检测扩增产物的条件下进行。 本文提供了用于检测HIV-1整合酶核酸（例如SEQ ID NO：8-14或8-27）和HIV-1逆转录酶核酸（例如SEQ ID NO：1-7）的LAMP引物组 。

    HIV PATHOLOGY MARKER AND EXAMINATION METHOD
    9.
    发明公开
    HIV PATHOLOGY MARKER AND EXAMINATION METHOD 审中-公开
    HIV病理标志物和检测方法

    公开(公告)号：EP3214182A1

    公开(公告)日：2017-09-06

    申请号：EP15854844.6

    申请日：2015-10-28

    申请人： Keio University ,  Nikon Corporation

    发明人： KATO, Shingo

    IPC分类号： C12Q1/68 ,  C12N15/09 ,  G01N33/15 ,  G01N33/50

    CPC分类号： C12Q1/703 ,  C12N15/09 ,  C12Q1/68 ,  C12Q1/686 ,  C12Q2600/106 ,  C12Q2600/16 ,  G01N33/15 ,  G01N33/50 ,  C12Q2537/143

    摘要： A technique for detecting intact provirus more simply and at a lower cost is provided. A marker showing HIV pathology, comprising near-full-length HIV provirus. A method of testing HIV pathology, comprising evaluating the level of full-length HIV provirus in HIV-infected cells. A method of testing the efficacy of an anti-HIV drug, comprising evaluating the level of near-full-length HIV provirus in HIV-infected cells. A method of screening for drugs that enable a functional cure of HIV, comprising evaluating the level of near-full-length HIV provirus in HIV-infected cells. A method of evaluating the level of near-full-length HIV provirus in HIV-infected cells, comprising detecting 3 to 7 regions scattered across the full length of HIV provirus. Also provided is a kit for evaluating the level of near-full-length HIV provirus, comprising primers capable of amplifying 3 to 7 regions scattered across the full length of HIV provirus and probes capable of detecting the nucleic acid amplified by the primers.

    摘要翻译： 提供了一种更简单，更低成本检测完整原病毒的技术。 显示HIV病理学的标记，包含接近全长的HIV原病毒。 测试HIV病理的方法，包括评估HIV感染细胞中全长HIV前病毒的水平。 测试抗HIV药物功效的方法，包括评估HIV感染细胞中接近全长HIV原病毒的水平。 筛选能够实现HIV功能治愈的药物的方法，包括评估HIV感染细胞中接近全长HIV原病毒的水平。 一种评估HIV感染细胞中接近全长HIV原病毒水平的方法，包括检测散布在整个HIV原病毒中的3至7个区域。 还提供了用于评估近全长HIV原病毒水平的试剂盒，其包含能够扩增遍及HIV原病毒全长的3至7个区域的引物和能够检测由引物扩增的核酸的探针。