专利检索 ap:("AMBION, INC.") AND inv:"BYROM, Mike" 第 1 页

1.

发明公开
METHODS AND COMPOSITIONS RELATING TO POLYPEPTIDES WITH RNASE III DOMAINS THAT MEDIATE RNA INTERFERENCE 审中-公开
标题翻译：方法和组合物与之相关多肽用RNase III域，RNA干扰TRANSFER

公开(公告)号：EP1532271A2

公开(公告)日：2005-05-25

申请号：EP03741956.1

申请日：2003-06-12

申请人： AMBION, INC.

发明人： FORD, Lance, P. , BYROM, Mike , PASLOSKE, Brittan, L. , BROWN, David

IPC分类号： C12Q1/68 , A01N43/04 , C07H21/04 , A61K31/07

CPC分类号： C12N15/1135 , C12N9/22 , C12N15/111 , C12N15/113 , C12N15/1136 , C12N15/1137 , C12N2310/14 , C12N2330/30 , C12Y301/26003

摘要： The present invention concerns methods and compositions involving RNase III and polypeptides containing RNase III domains to generate RNA capable of triggering RNA-mediated interference (RNAi) in a cell. In some embodiments, the RNase III is from a prokaryote. RNase III activity will cleave a double-stranded RNA molecule into short RNA molecules that may trigger or mediate RNAi (siRNA). Compositions of the invention include kits that include an RNase III domain-containing polypeptide. The present invention further concerns methods using polypeptides with RNase III activity for generating RNA molecules that effect RNAi, including the generation of a number of RNA molecules to the same target.