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1.发明公开
公开(公告)号：EP1360333A2
公开(公告)日：2003-11-12
申请号：EP02720894.1
申请日：2002-01-31
申请人： AMBION, INC.
发明人： WINKLER, Matthew, M. , BROWN, David
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6809 , C12Q2531/143 , C12Q2525/161 , C12Q2525/155
摘要： Disclosed are methods that allow one or more nucleic acid targets to be compared across two or more nucleic acid samples. Nucleic acid tags are appended to the samples to be assessed, such that each sample has a unique tag. The tagged nucleic acids are then mixed, and the targets within the mixture are amplified. The amplification products are distinguished using the unique tag domains to reveal the abundance of the amplification products derived from each sample, which correlates to the relative abundance of the target in the samples.
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公开(公告)号：EP1003914A1
公开(公告)日：2000-05-31
申请号：EP98938321.1
申请日：1998-08-05
申请人： AMBION, INC.
发明人： BROWN, David , WINKLER, Matthew
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6834 , C12Q2565/125 , C12Q2527/125 , C12Q2525/185 , C12Q2521/301
摘要： The present invention is directed to compositions and methods for removal of nucleic acid probes from sample nucleic acids, particularly when the sample nucleic acids are attached to a solid support. The invention also concerns methods of stripping and reusing nucleic acid blots.
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公开(公告)号：EP1771563A2
公开(公告)日：2007-04-11
申请号：EP05804851.3
申请日：2005-05-31
申请人： AMBION, INC.
发明人： BROWN, David , CONRAD, Rick , DEVROE, Eric , GOLDRICK, Marianna , KEIGER, Kerri , LABOURIER, Emmanuel , MOON, Ivonne , POWERS, Patricia , SHELTON, Jeffrey , SHINGARA, Jaclyn
IPC分类号： C12N15/11
CPC分类号： C12Q1/6806 , C12N15/111 , C12N2310/14 , C12N2310/3517 , C12N2310/3519 , C12N2320/10 , C12P19/34 , C12Q1/6809 , C12Q1/6883 , C12Q2600/158 , C12Q2600/178 , C12Q2565/501 , C12Q2525/207
摘要： The present invention concerns methods and compositions for isolating, enriching, and/or labeling miRNA molecules and for preparing and using arrays or other detection techniques for miRNA analysis. Moreover, the present invention concerns methods and compositions for generating miRNA profiles and employing such profiles for therapeutic, diagnostic, and prognostic applications.
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4.发明公开METHODS AND COMPOSITIONS RELATING TO POLYPEPTIDES WITH RNASE III DOMAINS THAT MEDIATE RNA INTERFERENCE 审中-公开方法和组合物与之相关多肽用RNase III域，RNA干扰TRANSFER
公开(公告)号：EP1532271A2
公开(公告)日：2005-05-25
申请号：EP03741956.1
申请日：2003-06-12
申请人： AMBION, INC.
CPC分类号： C12N15/1135 , C12N9/22 , C12N15/111 , C12N15/113 , C12N15/1136 , C12N15/1137 , C12N2310/14 , C12N2330/30 , C12Y301/26003
摘要： The present invention concerns methods and compositions involving RNase III and polypeptides containing RNase III domains to generate RNA capable of triggering RNA-mediated interference (RNAi) in a cell. In some embodiments, the RNase III is from a prokaryote. RNase III activity will cleave a double-stranded RNA molecule into short RNA molecules that may trigger or mediate RNAi (siRNA). Compositions of the invention include kits that include an RNase III domain-containing polypeptide. The present invention further concerns methods using polypeptides with RNase III activity for generating RNA molecules that effect RNAi, including the generation of a number of RNA molecules to the same target.
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公开(公告)号：EP1572902A3
公开(公告)日：2005-10-12
申请号：EP03708928.1
申请日：2003-01-31
申请人： AMBION, INC.
IPC分类号： C12N1/00
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公开(公告)号：EP1572902A2
公开(公告)日：2005-09-14
申请号：EP03708928.1
申请日：2003-01-31
申请人： AMBION, INC.
IPC分类号： C12N1/00
CPC分类号： C12N15/113 , C12N15/111 , C12N15/1135 , C12N15/1137 , C12N2310/111 , C12N2310/14 , C12N2310/322 , C12N2310/333 , C12N2310/334 , C12N2310/335 , C12N2310/336 , C12N2310/53 , C12N2320/50 , C12Y102/01012
摘要： The present invention provides improved methods of attenuating gene expression through the phenomenon of RNA interference. The invention provides methods of synthesis of double stranded RNAs (dsRNAs) of increased potency for use as small interfering RNA (siRNA). Surprisingly and unexpectedly, siRNAs made by the methods of the invention are significantly more potent than previously available siRNAs.
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