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公开(公告)号：EP0668929B1
公开(公告)日：1997-08-13
申请号：EP92911283.7
申请日：1992-06-05
发明人： BLAIR, James, Ninian, Rowell 39 Old Church Road , DOWNES, Malcolm, John , PARRY, David, Alun Flat 2 , CROSS, Lisa , ROBINSON, Philip, Steven 98 Winslow Road Wingrave
CPC分类号： C12Q1/6818 , C12Q1/6851 , C12Q2563/131 , C12Q2563/101 , C12Q2545/113
摘要： A method of amplifying and quantitating a specific nucleic acid sequence in a sample using at least one oligonucleotide primer, a supply of nucleotides and optionally a probe complementary to part of the sequence, one of these including a radionuclide. In the presence of a polymerase the reagents form an amplified product based on the specific nucleic acid sequence. The amplified sequence is allowed to bind to a solid material comprising a fluorescer which fluoresces in proximity to the radionuclide and fluorescence is observed as a measure of the specific nucleic acid sequence. The method can be applied to a range of well-known nucleic acid amplification techniques such as the Polymerase Chain Reaction. A variation on the above method allows use of the Ligase Chain Reaction technique.
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公开(公告)号：EP0668929A1
公开(公告)日：1995-08-30
申请号：EP92911283.0
申请日：1992-06-05
发明人： BLAIR, James, Ninian, Rowell 39 Old Church Road , DOWNES, Malcolm, John , PARRY, David, Alun Flat 2 , CROSS, Lisa , ROBINSON, Philip, Steven 98 Winslow Road Wingrave
IPC分类号： C12Q1
CPC分类号： C12Q1/6818 , C12Q1/6851 , C12Q2563/131 , C12Q2563/101 , C12Q2545/113
摘要： A method of amplifying and quantitating a specific nucleic acid sequence in a sample using at least one oligonucleotide primer, a supply of nucleotides and optionally a probe complementary to part of the sequence, one of these including a radionuclide. In the presence of a polymerase the reagents form an amplified product based on the specific nucleic acid sequence. The amplified sequence is allowed to bind to a solid material comprising a fluorescer which fluoresces in proximity to the radionuclide and fluorescence is observed as a measure of the specific nucleic acid sequence. The method can be applied to a range of well-known nucleic acid amplification techniques such as the Polymerase Chain Reaction. A variation on the above method allows use of the Ligase Chain Reaction technique.
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